SF001-500P ESGRO Complete PLUS Clonal Grade Medium, 500ml

SF001-500P
500 mL  
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      Cult
      Description
      Catalogue NumberSF001-500P
      ReplacesSF001-500
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionESGRO Complete PLUS Clonal Grade Medium, 500ml
      OverviewESGRO Complete PLUS Clonal Grade Medium is a defined serum-free medium provided with a selective GSK3β inhibitor to enhance viability of mouse embryonic stem (ES) cells and increased maintenance of pluripotency in the absence of serum and feeder cells. This medium is intended for mouse ES cell expansion and growth at clonal density in serum-free conditions. The mouse ES cells should maintain germ line competency.
      References
      Product Information
      Components
      • 1. ESGRO Complete Clonal Grade Medium: (Catalogue No. SF001-B) One (1) 500 mL bottle containing original clonal grade medium. Complete formulation containing self-renewal factors, no need to add LIF. pH 7.1-7.3
      • 2. GSK3β Inhibitor Supplement: (Catalogue No. SF012-250) One (1) 250 µL vial containing selective GSK3β inhibitor.
      Applications
      ApplicationThe ESGRO Complete PLUS Clonal Grade Medium is a defined serum-free medium provided with a selective GSK3β inhibitor to enhance viability of mouse embryonic stem cells & increased maintenance of pluripotency in the absence of serum & feeder cells.
      Key Applications
      • Stem Cell Culture
      Application NotesSuitable for ES cell derivation, transfection and selection, in addition to general cell passaging and growth.
      Media can be used on feeder dependent/independent ES cell lines.

      Important passaging information
      Use of one of the below listed cell dissociation solutions is imperative while passaging ES cells using ESGRO Complete products. Standard trypsin protocols used to passage ES cells grown in serum containing media typically result in over trypsinization causing the cells to lift off the plates and form abnormal ball-like structures.
      Recommended products include:
      ESGRO Complete™ Accutase (Cat # SF006)
      ESGRO Complete™ Trypsin Solution (proprietary) (Cat # SF007)
      ESGRO Complete™ Enzyme-Free Dissociation Solution (proprietary) (Cat # SF009)
      Biological Information
      Media FormLiquid
      Stem Cell Type
      • Mouse Embryonic Stem Cells
      • Induced Pluripotent Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceQC PROTOCOL:

      Low density plating assay:
      1. Plate feeder independent early passage ES cells onto gelatin coated 10 cm plastic tissue culture dish at 1000 cells/10 cm dish in 10-20 mL of Clonal Grade Medium.
      2. Change medium every 2-4 days.
      3. Monitor for clonal growth with alkaline phosphatase stain.

      Colony formation should occur over 4-6 days. The majority of colonies should be alkaline phosphatase positive and show no signs of differentiation.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions• Upon receipt, store clonal grade medium and supplement in the dark at -20°C. Refer to lot expiration date on labels.
      • Thawed clonal grade medium should be stored in the dark at 4°C for one month. Do not refreeze.
      • Supplement may be thawed at room temperature, aliquoted and stored in the dark at 4°C for two months.
      • After supplement is added to clonal grade medium, store in the dark at 4°C for up to
      two weeks. Do not refreeze.
      Packaging Information
      Material Size500 mL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      ESGRO Complete PLUS Clonal Grade Medium, 500ml SDS

      Title

      Safety Data Sheet (SDS) 

      ESGRO Complete PLUS Clonal Grade Medium, 500ml Certificates of Analysis

      TitleLot Number
      ESGRO COMPLETE™ PLUS SERUM-FREE CLONAL GRADE MEDIUM3054097
      ESGRO COMPLETE™ PLUS SERUM-FREE CLONAL GRADE MEDIUM - 24609402460940
      ESGRO COMPLETE PLUS SERUM-FREE CLONAL GRADE MEDIUM - 21082172108217
      ESGRO COMPLETE PLUS SERUM-FREE CLONAL GRADE MEDIUM - 21876892187689
      ESGRO COMPLETE PLUS SERUM-FREE CLONAL GRADE MEDIUM -
      ESGRO COMPLETE PLUS SERUM-FREE CLONAL GRADE MEDIUM - 11212-311212-3
      ESGRO COMPLETE PLUS SERUM-FREE CLONAL GRADE MEDIUM - 19773591977359
      ESGRO COMPLETE PLUS SERUM-FREE CLONAL GRADE MEDIUM - 21522172152217
      ESGRO COMPLETE PLUS SERUM-FREE CLONAL GRADE MEDIUM - 21961272196127
      ESGRO COMPLETE PLUS SERUM-FREE CLONAL GRADE MEDIUM - 22094452209445

      References

      Reference overviewPub Med ID
      Dynamic transcriptional events in embryonic stem cells mediated by the super elongation complex (SEC).
      Lin C. et al.
      Genes Dev.  25(14)  1486-1498  2011

      Show Abstract
      21764852 21764852
      HDAC1 regulates pluripotency and lineage specific transcriptional networks in embryonic and trophoblast stem cells.
      Kidder B. L. & Palmer S.
      Nucleic Acids Res.  1-15  2011

      Show Abstract
      22156375 22156375
      A critical appraisal of factors affecting the accuracy of results obtained when using flow cytometry in stem cell investigations: where do you put your gates?
      Hughes O. R. et al.
      Cytometry A  75(9)  803-810  2009

      Show Abstract
      19562683 19562683
      Optimized mouse ES cell culture system by suspension growth in a fully defined medium.
      Andäng M. et al.
      Nat. Protoc,  3(6)  1013-1017  2008

      Show Abstract
      18536648 18536648

      Data Sheet

      Title
      ESGRO COMPLETE™ PLUS SERUM-FREE CLONAL GRADE MEDIUM