328005 | ERK Activation Inhibitor Peptide II, Cell-Permeable - Calbiochem

328005
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical Formula
      C₁₃₆H₂₄₁N₅₃O₃₁S

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      328005-1MG
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          Plastic ampoule 1 mg
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          Description
          OverviewA cell-permeable and reversible 13-amino acid peptide corresponding to the N-terminus of MEK1 (MAPKK) that is fused to the HIV-TAT membrane translocating peptide (MTP) sequence via a glycine linker. Acts as a specific inhibitor of ERK activation and blocks the transcriptional activity of Elk1. Selectively binds to ERK2 and prevents its interaction with MEK (IC50 = 210 nM). Potently blocks ERK activation in PMA-stimulated NIH 3T3 cells and NGF-treated PC12 cells (IC50 = 29 µM). Does not affect the activation of JNKs or p38.
          Catalogue Number328005
          Brand Family Calbiochem®
          SynonymsMTPTAT-G-MEK1₁₃, H-GYGRKKRRQRRR-G-MPKKKPTPIQLNP-NH₂, Erk Inhibitor V
          References
          ReferencesKelemen, B.R., et al. 2002. J. Biol. Chem. 277, 8741.
          Product Information
          ATP CompetitiveN
          FormLyophilized solid
          FormulationSupplied as a trifluoroacetate salt.
          Hill FormulaC₁₃₆H₂₄₁N₅₃O₃₁S
          Chemical formulaC₁₃₆H₂₄₁N₅₃O₃₁S
          Hygroscopic Hygroscopic
          ReversibleY
          Applications
          Biological Information
          Primary TargetMEK
          Primary Target IC<sub>50</sub>210 nM against MEK; 29 µM in blocking ERK activation in PMA-stimulated NIH 3T3 cells and NGF-treated PC12 cells
          Purity≥95% by HPLC
          Physicochemical Information
          Cell permeableY
          Peptide SequenceH-Gly-Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Gly-Met-Pro-Lys-Lys-Lys-Pro-Thr-Pro-Ile-Gln-Leu-Asn-Pro-NH₂
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Protect from Light Protect from light
          Hygroscopic Hygroscopic
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          328005

          References

          Reference overview
          Kelemen, B.R., et al. 2002. J. Biol. Chem. 277, 8741.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision04-January-2010 RFH
          SynonymsMTPTAT-G-MEK1₁₃, H-GYGRKKRRQRRR-G-MPKKKPTPIQLNP-NH₂, Erk Inhibitor V
          DescriptionA cell-permeable and reversible 13-amino acid peptide corresponding to the N-terminus of MAPK/ERK kinase (MEK or MAPKK) that is fused to the HIV-TAT membrane translocation peptide (MTP) sequence via a glycine linker. Acts as a specific inhibitor of ERK activation by MEK1 and the downstream transcriptional activity of ELK1. Selectively binds to ERK2 and prevents its interaction with MEK (IC50 = 210 nM in vitro). Potently blocks ERK in PMA-stimulated NIH3T3 cells and NGF-treated PC12 cells (IC50 = 29 µM). Does not inhibit the activation of c-jun N-terminal kinases (JNKs) or p38 MAP kinase.
          FormLyophilized solid
          FormulationSupplied as a trifluoroacetate salt.
          Intert gas (Yes/No) Packaged under inert gas
          Chemical formulaC₁₃₆H₂₄₁N₅₃O₃₁S
          Peptide SequenceH-Gly-Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Gly-Met-Pro-Lys-Lys-Lys-Pro-Thr-Pro-Ile-Gln-Leu-Asn-Pro-NH₂
          Purity≥95% by HPLC
          SolubilityH₂O (1 mg/ml)
          Storage -20°C
          Hygroscopic
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Toxicity Standard Handling
          ReferencesKelemen, B.R., et al. 2002. J. Biol. Chem. 277, 8741.