L2113-BC EDTA (Versen), 1 %

L2113-BC
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      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      L2113
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          100 ml
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          Description
          OverviewTrypsin/EDTA solutions (0.05 or 0.02% in PBS, without Ca2+, Mg2+) are used for passaging monolayer cultures. Trypsin affects the cell membrane and the cellular, and also the intercellular mucoproteins. Trypsin can be inhibited with the use of serum.

          Recommendation Instructions:

          Recommendations Trypsin (1:250)/EDTA solutions:
          Trypsin (1:250)/EDTA solutions (0.05/0.02 % in PBS without Ca2+, Mg2+) to passage monolayer cultures:
          1. Wash cell culture (monolayer) using physiological saline (w/o Ca2+, Mg2+).
          2. Apply a small quantity of Trypsin (1:250)/EDTA solution with a pipette to cell culture flask; a thin film is sufficient.
          3. Incubate culture flask at +37°C for approx. 5 min.
          4. Centrifuge cell suspension or transfer directly to a serum containing culture medium.

          With higher Trypsin concentrations, it is recommended to trypsinate at +2-+8°C.
          Specifications:

          Functional Test: Pass
          Activity Test: Pass
          pH: 7.2-7.5
          Osmolality: 0.27-0.30 Osm/kg H20
          Color: Colorless
          Appearance: Clear

          Catalogue NumberL2113-BC
          Brand Family Calbiochem®
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          Ship Code Blue Ice Only
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
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