371208 Dkk-1, Rat, Recombinant

371208
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service
      View Pricing & Availability
      Click To Print This Page

      Overview

      Replacement Information

      Key Specifications Table

      Purity
      ≥95% by SDS-PAGE

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      371208-10UG
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          Glass bottle 10 μg
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          OverviewRecombinant, rat Dickkopf related protein-1 (Dkk-1), consisting of amino acids 23-270, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-derived mouse myeloma cells. The balance between Wnt signaling and Dkk-1 inhibition is essential for bone formation and homeostasis.
          Catalogue Number371208
          Brand Family Calbiochem®
          References
          ReferencesLi, J. et al. 2006. Bone 36, 754.
          Morvan, F. et al. 2006. J. Bone Miner. Res. 21, 934.
          Niehrs, C. 2006. Oncogene 25, 7469.
          Verani, R. et al. 2006. J. Neurochem. 101, 242.
          Cappuccio, I. et al. 2005. J. Neurosci. 25, 2647.
          Kemp, C. et al. 2005. Dev. Dyn. 233, 1064.
          Adamska, M. et al. 2004. Dev. Biol. 272, 134.
          Bullock, C.M. et al. 2004. Mol. Pharmacol. 65, 582.
          Mao, B. et al. 2002. Nature 417, 664.
          Mao, B. et al. 2001. Nature 411, 321.
          Krupnik, V.E., et al. 1999. Gene 238, 301.
          Product Information
          FormLyophilized
          FormulationLyophilized from 0.2 µm filtered PBS, BSA.
          Applications
          Biological Information
          Biological activityND₅₀ = 0.1-0.5 µg/ml as measured by inhibition of Wnt-3a-induced (10 ng/ml rmWnt-3a) alkaline phosphatase production by proliferation of MC3T3-E1 mouse osteoblastic cells.
          Purity≥95% by SDS-PAGE
          Physicochemical Information
          ContaminantsEndotoxin: ≤1.0 EU/µg protein
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          371208

          References

          Reference overview
          Li, J. et al. 2006. Bone 36, 754.
          Morvan, F. et al. 2006. J. Bone Miner. Res. 21, 934.
          Niehrs, C. 2006. Oncogene 25, 7469.
          Verani, R. et al. 2006. J. Neurochem. 101, 242.
          Cappuccio, I. et al. 2005. J. Neurosci. 25, 2647.
          Kemp, C. et al. 2005. Dev. Dyn. 233, 1064.
          Adamska, M. et al. 2004. Dev. Biol. 272, 134.
          Bullock, C.M. et al. 2004. Mol. Pharmacol. 65, 582.
          Mao, B. et al. 2002. Nature 417, 664.
          Mao, B. et al. 2001. Nature 411, 321.
          Krupnik, V.E., et al. 1999. Gene 238, 301.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision22-June-2011 RFH
          DescriptionRecombinant, rat Dickkopf related protein-1 (Dkk-1), consisting of amino acids 23-270, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-derived mouse myeloma cells. The balance between Wnt signaling and Dkk-1 inhibition is essential for bone formation and homeostasis.
          FormLyophilized
          FormulationLyophilized from 0.2 µm filtered PBS, BSA.
          Purity≥95% by SDS-PAGE
          ContaminantsEndotoxin: ≤1.0 EU/µg protein
          Biological activityND₅₀ = 0.1-0.5 µg/ml as measured by inhibition of Wnt-3a-induced (10 ng/ml rmWnt-3a) alkaline phosphatase production by proliferation of MC3T3-E1 mouse osteoblastic cells.
          SolubilityReconstitute to 100 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
          Toxicity Standard Handling
          ReferencesLi, J. et al. 2006. Bone 36, 754.
          Morvan, F. et al. 2006. J. Bone Miner. Res. 21, 934.
          Niehrs, C. 2006. Oncogene 25, 7469.
          Verani, R. et al. 2006. J. Neurochem. 101, 242.
          Cappuccio, I. et al. 2005. J. Neurosci. 25, 2647.
          Kemp, C. et al. 2005. Dev. Dyn. 233, 1064.
          Adamska, M. et al. 2004. Dev. Biol. 272, 134.
          Bullock, C.M. et al. 2004. Mol. Pharmacol. 65, 582.
          Mao, B. et al. 2002. Nature 417, 664.
          Mao, B. et al. 2001. Nature 411, 321.
          Krupnik, V.E., et al. 1999. Gene 238, 301.