371206 | Dkk-1, His•Tag®, Mouse, Recombinant

371206
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      Overview

      Replacement Information

      Key Specifications Table

      Purity
      ≥95% by SDS-PAGE

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      371206-10UG
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      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service

          Glass bottle 10 μg
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          Description
          OverviewRecombinant, murine Dickkopf related protein-1 (Dkk-1), consisting of amino acids 30-272, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-dervied mouse myeloma cells. Dkk-1 is a well-documented antagonist of the Wnt signaling pathway. The balance between Dkk-1 inhibition and Wnt signaling is essential for bone formation and homeostasis.
          Catalogue Number371206
          Brand Family Calbiochem®
          References
          ReferencesPinzone, J.J. et al. 2009. Blood 113, 517.
          Li, J. et al. 2006. Bone 36, 754.
          Morvan, F. et al. 2006. J. Bone Miner. Res. 21, 934.
          Niehrs, C. 2006. Oncogene 25, 7469.
          Verani, R. et al. 2006. J. Neurochem. 101, 242.
          Cappuccio, I. et al. 2005. J. Neurosci. 25, 2647.
          Kemp, C. et al. 2005. Dev. Dyn. 233, 1064.
          Adamska, M. et al. 2004. Dev. Biol. 272, 134.
          Bullock, C.M. et al. 2004. Mol. Pharmacol. 65, 582.
          Mao, B. et al. 2002. Nature 417, 664.
          Mao, B. et al. 2001. Nature 411, 321.
          Glinka, A. et al. 1998. Nature 391, 357.
          Product Information
          FormLyophilized
          FormulationLyophilized from 0.2 µm filtered PBS, BSA.
          Applications
          Biological Information
          Biological activityND₅₀: 0.2-1 µg/ml as measured by the inhibition of Wnt-3a-induced alkaline phosphatase production in MC3T3-E1 mouse osteoblastic cells.
          Reduction of Axis-inducing activity: 60-100% reduction (using 1 ng rmDkk-1 per embryo) in the number of secondary axes produced in the presence of 10-20 pg Xwnt-8 mRNA using early Xenopus embryos.
          Purity≥95% by SDS-PAGE
          Physicochemical Information
          ContaminantsEndotoxin: ≤1.0 EU/µg protein
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Regulatory Review
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Certificates of Analysis

          TitleLot Number
          371206

          References

          Reference overview
          Pinzone, J.J. et al. 2009. Blood 113, 517.
          Li, J. et al. 2006. Bone 36, 754.
          Morvan, F. et al. 2006. J. Bone Miner. Res. 21, 934.
          Niehrs, C. 2006. Oncogene 25, 7469.
          Verani, R. et al. 2006. J. Neurochem. 101, 242.
          Cappuccio, I. et al. 2005. J. Neurosci. 25, 2647.
          Kemp, C. et al. 2005. Dev. Dyn. 233, 1064.
          Adamska, M. et al. 2004. Dev. Biol. 272, 134.
          Bullock, C.M. et al. 2004. Mol. Pharmacol. 65, 582.
          Mao, B. et al. 2002. Nature 417, 664.
          Mao, B. et al. 2001. Nature 411, 321.
          Glinka, A. et al. 1998. Nature 391, 357.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision22-June-2011 RFH
          DescriptionRecombinant, murine Dickkopf related protein-1 (Dkk-1), consisting of amino acids 30-272, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-dervied mouse myeloma cells. Dkk-1 is a well-documented antagonist of the Wnt signaling pathway. The balance between Dkk-1 inhibition and Wnt signaling is essential for bone formation and homeostasis.
          FormLyophilized
          FormulationLyophilized from 0.2 µm filtered PBS, BSA.
          Purity≥95% by SDS-PAGE
          ContaminantsEndotoxin: ≤1.0 EU/µg protein
          Biological activityND₅₀: 0.2-1 µg/ml as measured by the inhibition of Wnt-3a-induced alkaline phosphatase production in MC3T3-E1 mouse osteoblastic cells.
          Reduction of Axis-inducing activity: 60-100% reduction (using 1 ng rmDkk-1 per embryo) in the number of secondary axes produced in the presence of 10-20 pg Xwnt-8 mRNA using early Xenopus embryos.
          SolubilityReconstitute to 100 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
          Toxicity Regulatory Review
          ReferencesPinzone, J.J. et al. 2009. Blood 113, 517.
          Li, J. et al. 2006. Bone 36, 754.
          Morvan, F. et al. 2006. J. Bone Miner. Res. 21, 934.
          Niehrs, C. 2006. Oncogene 25, 7469.
          Verani, R. et al. 2006. J. Neurochem. 101, 242.
          Cappuccio, I. et al. 2005. J. Neurosci. 25, 2647.
          Kemp, C. et al. 2005. Dev. Dyn. 233, 1064.
          Adamska, M. et al. 2004. Dev. Biol. 272, 134.
          Bullock, C.M. et al. 2004. Mol. Pharmacol. 65, 582.
          Mao, B. et al. 2002. Nature 417, 664.
          Mao, B. et al. 2001. Nature 411, 321.
          Glinka, A. et al. 1998. Nature 391, 357.