69725 λDE3 Lysogenization Kit plus pLysS & pLysE - Novagen

69725
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      Overview

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          Glass bottle 10 rxn
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          Description
          OverviewThe λDE3 Lysogenization Kit is designed for site-specific integration of λDE3 prophage into an E. coli host cell chromosome, such that the lysogenized host can be used to express target genes cloned in pET vectors. λDE3 is a recombinant phage carrying the cloned gene for T7 RNA polymerase under lacUV5 control. Lysogens are prepared by co-infection with the three provided phages, and can be verified by plating the T7 Tester Phage (a T7 RNA polymerase deletion mutant). This phage is unable to make a plaque on cells that lack T7 RNA polymerase, but makes normal plaques on λDE3 lysogens in the presence of IPTG.The T7 RNA Polymerase Monoclonal Antibody can be used to verify the presence of T7 RNA polymerase in the host strain by Western blot analysis.

          This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
          Catalogue Number69725
          Brand Family Novagen®
          References
          Product Information
          200 µlλDE3 Phage Lysate
          200 µlHelper Phage Lysate
          200 µlSelection Phage Lysate
          50 µlT7 Tester Phage Lysate
          200 µlHMS174(DE3) glycerol stock
          1 µgpLysS DNA
          1 µgpLysE DNA
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          λDE3 Lysogenization Kit plus pLysS & pLysE - Novagen SDS

          Title

          Safety Data Sheet (SDS) 

          λDE3 Lysogenization Kit plus pLysS & pLysE - Novagen Certificates of Analysis

          TitleLot Number
          69725

          Citations

          Title
        • Sandra Angelini, Sandra Deitermann and Hans-Georg Koch. (2005) FtsY, the bacterial signal-recognition particle receptor, interacts functionally and physically with the SecYEG translocon. European Molecular Biology Organization Reports 6, 476-481.
        • Junhua Zhao and Alan M. Lambowitz. (2005) A bacterial group II intron-encoded reverse transcriptase localizes to cellular poles. Proceedings of the National Academy of Sciences (USA) 102, 16133-16140.
        • Dhammika Gunasekera and Robert G. Kemp. (1999) Cloning, sequencing, expression, and purification of the C isozyme of mouse phosphofructokinase. Protein Expression and Purification 16, 448-453.
        • Vector Map

          Title
          TB107 pLysS and pLysE Plasmid Map

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          Categories

          Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors