70526 Clonables™ Ligation/Transformation Kit - Novagen

70526
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      Overview

      Replacement Information

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      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      70526-3
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          Plastic ampoule 11 rxn
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          Description
          OverviewThe Clonables™ Kit enables convenient, dependable, high-efficiency ligation and transformation of any compatible DNA ends. The kit features a unique, universal Ligation Premix, containing ligase, buffer, and cofactors, which supports ligation of any type of DNA cohesive or blunt ends in a 15-minute reaction. Ligated DNA is transformed into NovaBlue Singles™ Competent Cells, which use a streamlined protocol that takes less than 8 minutes for ampicillin-resistant plasmids and 38 minutes for other antibiotic-resistant plasmids. This kit can be used with a variety of cloning vectors and is compatible with any type of DNA end, without altering the ends or desired cloning junctions.

          The Clonables Kit contains sufficient reagents to perform 11 ligation and transformation reactions, and includes a control vector and insert mix to verify performance.
          Catalogue Number70526
          Brand Family Novagen®
          Features and benefits
        • Fast! 15-minute ligation, 8-minute transformation with ampicillin selection
        • Ligation components premixed to decrease pipetting steps and increase reliability
        • One reaction, optimized for cohesive ends, single base overhangs, and blunt ends
        • Single-use competent cells eliminate need to aliquot, freeze/thaw, or waste partially used vials
        • Compatible with PCR buffer, TE, restriction enzyme buffer, or End Conversion Mix
        • References
          Product Information
          Components
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Clonables™ Ligation/Transformation Kit - Novagen SDS

          Title

          Safety Data Sheet (SDS) 

          Clonables™ Ligation/Transformation Kit - Novagen Certificates of Analysis

          TitleLot Number
          70526

          Citations

          Title
        • Sulene L. Chi, et al. (2007) Angiostatin-like activity of a monoclonal antibody to the catalytic subunit of F1F0 ATP synthase. Cancer Research 67, 4716-4724.
        • Bratislav Janjic, et al. (2006) Spontaneous CD4+ T cell responses against TRAG-3 in patients with melanoma and breast cancers. Journal of Immunology 177, 2717-2727.
        • User Protocols

          Title
          TB233 Clonables™ Kit