227039 | Cholera Toxin, B Subunit, Vibrio cholerae, Type Inaba 569B - Calbiochem

227039
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      227039-1MG
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          Alu drum 1 mg
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          Description
          OverviewSubunit of cholera toxin responsible for binding to GM1 ganglioside receptors in membranes. When used along, will block the action of toxin on intact cells.
          Catalogue Number227039
          Brand Family Calbiochem®
          References
          ReferencesMcCloskey, M.A. and Zhang, L. 2000. J. Cell. Biol. 148, 137.
          Mulhein, S.A., et al. 1989. J. Membr. Biol. 109, 21.
          Tsai, S.-C., et al. 1988. J. Biol. Chem. 263, 1768.
          Van Heynigen, S. 1982. Biosci. Rep. 2, 135.
          Mekalanos, J.J., et al. 1978. Infect. Immun. 20, 552.
          Gill, D.M. 1976. Biochemistry 15, 1242.
          Lai, C.Y., et al. 1976. J. Infect. Dis. 139, 674.
          Rappaport, R.S., et al. 1974. Infect. Immun. 9, 294.
          Craig, J.P. 1971. In Kadies, S., et al. (Eds.). Microbial Toxins. New York. Academic Press, 2A, 189.
          Product Information
          DeclarationNot available for sale outside of the United States.
          FormSolid
          FormulationLyophilized from 200 mM NaCl, 50 mM Tris-HCl, 3 mM NaN₃, 1 mM EDTA, pH 7.5.
          ReversibleN
          Applications
          ApplicationCholera Toxin, B Subunit, Vibrio cholerae, Type Inaba 569B, CAS 9012-63-9, is responsible for toxin binding to GM1 ganglioside receptors. When used alone, it blocks toxin action on intact cells.
          Biological Information
          Biological activityCholera Toxin, B Subunit is tested for its effectiveness in binding antitoxin using the antitoxin combining power assay described by Craig, J.P., et al.
          Primary TargetGM1 ganglioside receptors
          Physicochemical Information
          Cell permeableN
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          R PhraseR: 21/22-36/37/38

          Harmful in contact with skin and if swallowed.
          Irritating to eyes, respiratory system and skin.
          S PhraseS: 7-36/37

          Keep container tightly closed.
          Wear suitable protective clothing and gloves.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Highly Toxic
          Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
          Storage +2°C to +8°C
          Do not freeze Yes
          Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 6 months at 4°C. DO NOT FREEZE reconstituted solutions.
          End use certificateY
          Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          227039

          References

          Reference overview
          McCloskey, M.A. and Zhang, L. 2000. J. Cell. Biol. 148, 137.
          Mulhein, S.A., et al. 1989. J. Membr. Biol. 109, 21.
          Tsai, S.-C., et al. 1988. J. Biol. Chem. 263, 1768.
          Van Heynigen, S. 1982. Biosci. Rep. 2, 135.
          Mekalanos, J.J., et al. 1978. Infect. Immun. 20, 552.
          Gill, D.M. 1976. Biochemistry 15, 1242.
          Lai, C.Y., et al. 1976. J. Infect. Dis. 139, 674.
          Rappaport, R.S., et al. 1974. Infect. Immun. 9, 294.
          Craig, J.P. 1971. In Kadies, S., et al. (Eds.). Microbial Toxins. New York. Academic Press, 2A, 189.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision10-September-2008 JSW
          DescriptionCholera toxin is an oligomeric protein consisting of a single A subunit (M.W. 28,000) containing two polypeptide chains, A1 and A2 , and a B subunit (M.W. 55,000) containing five polypeptide chains. Cholera toxin is the pathogenic agent responsible for the symptoms of cholera. Cholera toxin is also a potent activator of adenylate cyclase that binds the ADP-ribosylation factor and modulates its activity. The A subunit catalyzes the ADP-ribosylation of GS that leads to the persistent activation of adenylate cyclase. The consequent increase in intracellular cAMP levels stimulates Cl- secretion and leads to severe dehydration. The B subunit binds specifically to GM1 ganglioside receptors, which are expressed ubiquitously in mammalian cells, and assists in the transport of the A subunit through the membrane. When used alone, the B subunit will block the action of toxin on intact cells. Purified B subunit is produced by a modification of the methods published by Lai, C.Y., et al.
          FormSolid
          FormulationLyophilized from 200 mM NaCl, 50 mM Tris-HCl, 3 mM NaN₃, 1 mM EDTA, pH 7.5.
          Biological activityCholera Toxin, B Subunit is tested for its effectiveness in binding antitoxin using the antitoxin combining power assay described by Craig, J.P., et al.
          SolubilitySterile dH₂O.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 6 months at 4°C. DO NOT FREEZE reconstituted solutions.
          Toxicity Highly Toxic
          ReferencesMcCloskey, M.A. and Zhang, L. 2000. J. Cell. Biol. 148, 137.
          Mulhein, S.A., et al. 1989. J. Membr. Biol. 109, 21.
          Tsai, S.-C., et al. 1988. J. Biol. Chem. 263, 1768.
          Van Heynigen, S. 1982. Biosci. Rep. 2, 135.
          Mekalanos, J.J., et al. 1978. Infect. Immun. 20, 552.
          Gill, D.M. 1976. Biochemistry 15, 1242.
          Lai, C.Y., et al. 1976. J. Infect. Dis. 139, 674.
          Rappaport, R.S., et al. 1974. Infect. Immun. 9, 294.
          Craig, J.P. 1971. In Kadies, S., et al. (Eds.). Microbial Toxins. New York. Academic Press, 2A, 189.

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          Categories

          Life Science Research > Inhibitors and Biochemicals