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Description
Overview
This product has been discontinued.
A cell-permeable tetracyclic quinone compound that acts as a specific, reversible, and non-competitive inhibitor of CerK (ceramide kinase) activity (IC50 = ~5 µM) with little effect against SPHK1/2 or DGKγ (IC50 > 100 µM). Shown to reduce cellular C1P (ceramide-1-phosphate) synthesis by 40% at ≥ 20 µM in a rat basophilic leukemia cell line RBL-2H3 and block CerK-mediated degranulation in both RBL-2H3 and murine BMMC (bone marrow-derived mast cells) in a dose-dependent manner. Exhibits no cytotoxic effect against RBL-2H3 even at concentrations as high as 100 µM.
Catalogue Number
219489
Brand Family
Calbiochem®
Synonyms
F-12509A Cyclic Product K1
References
References
Kim, J.W., et al. 2007. Biochim. Biophys. Acta1771, 1262. Kumada, H., et al. 2007. Biosci. Biotechnol. Biochem.71, 2581 Kim, J.W., et al. 2005. Biochim. Biophys. Acta1738, 82.
Product Information
CAS number
1258005-85-4
Declaration
Sold for research purposes only, pursuant to an agreement with Japan.
Form
Yellow powder
Hill Formula
C₂₁H₂₈O₄
Chemical formula
C₂₁H₂₈O₄
Structure formula Image
Applications
Biological Information
Purity
≥95% by NMR (mixture of enantiomers)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Standard Handling
Storage
-20°C
Protect from Light
Protect from light
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Ceramide Kinase Inhibitor, K1 Certificates of Analysis
Title
Lot Number
219489
References
Reference overview
Kim, J.W., et al. 2007. Biochim. Biophys. Acta1771, 1262. Kumada, H., et al. 2007. Biosci. Biotechnol. Biochem.71, 2581 Kim, J.W., et al. 2005. Biochim. Biophys. Acta1738, 82.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
31-March-2011 RFH
Synonyms
F-12509A Cyclic Product K1
Description
A cell-permeable tetracyclic quinone compound that acts as a specific, reversible, and non-competitive inhibitor of CerK (ceramide kinase) activity (IC50 ~5 µM) with little effect against SPHK1/2 or DGKγ (IC50 >100 µM). Shown to reduce cellular C1P (ceramide-1-phosphate) synthesis by 40% at ≥20 µM in a rat basophilic leukemia cell line RBL-2H3 and block CerK-mediated degranulation in both RBL-2H3 and murine BMMC (bone marrow-derived mast cells) in a dose-dependent manner. Exhibits no cytotoxic effect against RBL-2H3 even at concentrations as high as 100 µM.
Form
Yellow powder
Intert gas (Yes/No)
Packaged under inert gas
CAS number
1258005-85-4
Chemical formula
C₂₁H₂₈O₄
Structure formula
Purity
≥95% by NMR (mixture of enantiomers)
Solubility
Ethanol (20 mg/ml)
Storage
-20°C
Protect from light
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity
Standard Handling
References
Kim, J.W., et al. 2007. Biochim. Biophys. Acta1771, 1262. Kumada, H., et al. 2007. Biosci. Biotechnol. Biochem.71, 2581 Kim, J.W., et al. 2005. Biochim. Biophys. Acta1738, 82.