Centrifree® Ultrafiltration Device

Separate free from bound solutes

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Overview

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Centrifree Ultrafiltration Device Clear Sorting & Filtering
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4104Centrifree Ultrafiltration Device with Ultracel PL membrane 50 Show Pricing & Availability

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    Documentation

    References

    Reference overviewApplication
    Fractionation of canine serum magnesium.
    Schenck PA.,Vet Clin Pathol. 2005 Jun;34(2):137-9.
    Vet Clin Pathol. 2005 Jun;34(2):137-9.  2005

    The indirect effect of Tityus discrepans on rabbit pulmonary vasculature.
    Novoa, Eva, et al.
    Respiratory physiology & neurobiology, 134: 33-41 (2003)  2003

    Show Abstract
    Cell Culture
    Metabolic activity of cultured rat brainstem, hippocampal and spinal cord slices.
    Caroline A. Connelly, Lawrence C. Chen and Steven D. Colquhoun
    Journal of Neuroscience Methods 99 (1-2): 1-7  2000

    Cell Culture
    Phenylalanine and tyrosine quantification by stable isotope dilution liquid chromatography-mass spectrometry from filter paper blood spots
    Tuchman, M. McCann, M. T.
    Clinical Chemistry (Washington, D. C.) VO- 45 IS- 4 PG- 571-573 Apr 1999  1999

    Sample Prep
    Adaptation of total phenytoin reagent pack for measuring free phenytoin levels with the Abbott AxSYM immunoassay analyzer
    Jarzabek JI (REPRINT) ; Kampa IS
    Therapeutic drug monitoring 1999, v21, n1, p134-136  1999

    Effects of tryptophan depletion vs. catecholamine depletion in patients with seasonal affective disorder in remission with light therapy
    Neumeister, Alexander et al.
    Archives of general psychiatry, 1998  1998

    Circadian rhythm of free melatonin in human plasma
    Kennaway DJ (REPRINT) ; Voultsios A
    JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM, 1998, V83, N3 MAR, P1013-1015  1998

    Clinical Lab, Protein Binding
    Factors affecting the free plasma fraction of phenytoin in patients with epilepsy
    Jimenez MC; Duran JA (REPRINT) ; Abadin JA
    Clinical drug investigation, 1998, V15, P153-157  1998

    Clinical Lab
    Differential analysis of blood urea using combined automated ultrafiltration and solid phase extraction in on-line series
    Shintani H.,Journal of Liquid Chromatography and Related Technologies (2205-2210) 1998
    Journal of Liquid Chromatography and Related Technologies (2205-2210) 1998  1998

    Determination of the halothane metabolites trifluoroacetic acid and bromide in plasma and urine by ion chromatography
    Hankins, D. C. Kharasch, E. D.
    Journal of Chromatography, B: Biomedical Applications VO- 692 IS- 2 PG- 413-418, 9 May 1997  1997

    FAQ

    QuestionAnswer
    Can I use Centrifree with a swinging-bucket rotor?Yes, but the filtration rate will be only 30-50% of that produced with a fixed-angle rotor at the same g-force.
    What are the pore and retention characteristics of the YMT 30,000 molecular weight cut-off membrane for Centrifree micropartition device?The actual pore size is not measured. The membrane is characterized by measuring its retention of known protein markers of specific molecular weights. The given cut-off is a nominal measurement.
    Does Centrifree have a shelf life?Yes, one year.
    What is the maximum spinning speed of the Centrifree unit?Device limits are measured in g-force, not rpm. The maximum recommended g-force is 2000 × g.
    How much ultrafiltrate can I expect when using the Centrifree?For volumes less than 0.5 ml: For a sample volume of 1.0 ml: In 10 minutes: I 1/2 the initial volume approximately 200 µl In 30 minutes: 2/3 to 3/4 the initial volume approximately 450 µl
    What is the maximum process volume for the Centrifree device?Maximum volume is 1.0 ml.
    What molecular weight cut-off does the Centrifree come in?The Centrifree unit is available in a 30,000 MW cut-off.
    Can I reuse Centrifree?No, Centrifree is intended for one-time use only. Multiple use risks membrane failure.
    What is the minimum process volume for the Centrifree device?Minimum volume is 0.15 ml.
    What temperatures can Centrifree be spun at?Spin devices at 4-50°C. Store at room temperature.