219399 | Cathepsin D Substrate I - Calbiochem

219399
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      219399-10MG
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          Plastic ampoule 10 mg
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          Description
          OverviewUseful as a substrate for the determination of cathepsin D activity. Suitable for either colorimetric or fluorometric assay. Cathepsin D hydrolyzes the Phe-Phe bond. Requires dipeptidyl aminopeptidase II to free 4-methoxy-β-naphthylamine (4MeOβNA).
          Catalogue Number219399
          Brand Family Calbiochem®
          SynonymsBz-RGFFP-4-MeOβNA, HCl
          References
          ReferencesTakahashi, T., and Tang, J. 1981. Methods Enzymol. 80, 565.
          Smith, R.E., and Van Frank, R.M. 1975. in Lysosomes in Biology and Pathology 4 (Dingle, J.T., and Dean, R.T., eds.) p. 193, American Elsevier, Holland.
          Product Information
          ATP CompetitiveN
          FormWhite lyophilized solid
          Hill FormulaC₄₉H₅₅N₉O₇ · HCl
          Chemical formulaC₄₉H₅₅N₉O₇ · HCl
          ReversibleN
          Applications
          Biological Information
          Primary TargetSubstrate for the determination of cathepsin D activity
          Purity≥95% by HPLC
          Physicochemical Information
          Cell permeableN
          Emission max.
          Excitation max.
          Peptide SequenceBz-Arg-Gly-Phe-Phe-Pro-4MeOβNA, HCl
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Protect from Light Protect from light
          Protect from Moisture Protect from moisture
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          219399

          References

          Reference overview
          Takahashi, T., and Tang, J. 1981. Methods Enzymol. 80, 565.
          Smith, R.E., and Van Frank, R.M. 1975. in Lysosomes in Biology and Pathology 4 (Dingle, J.T., and Dean, R.T., eds.) p. 193, American Elsevier, Holland.

          Brochure

          Title
          Cathepsins and Related Products Technical Bulletin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision05-June-2008 RFH
          SynonymsBz-RGFFP-4-MeOβNA, HCl
          DescriptionA useful substrate for colorimetric or fluorometric assay of cathepsin D activity. Cathepsin D hydrolyzes the Phe-Phe bond. Dipeptidyl aminopeptidase II is required to free the 4-methoxy-β-naphthylamine (4MβNA). Excitation max: ~345 nm; emission max: ~425 nm.
          FormWhite lyophilized solid
          Recommended reaction conditions
          1. Incubate the substrate, Bz-Arg-Gly-Phe-Phe-Pro-4MβNA (final concentration 350 µM) with cathepsin D in 500 µl of reaction buffer (20 mM glycine-HCl, pH 3.0) at 37°C to allow digestion of the substrate to Bz-Arg-Gly-Phe-OH and Phe-Pro-4MβNA. 2. Stop the reaction by adding 50 µl of 1% KOH and then add 850 µL of reaction buffer 2 (100 mM Tris-HCl, pH 7.8). 3. Add 1 mU dipeptidylpeptidase IV in 100 µl of reaction buffer 2 and incubate for >1 h at 37°C to completely hydrolyze the Phe-Pro-4MβNA to Phe-Pro-OH and 4MβNA. 4. Monitor the release of 4MβNA at 345 nm (excitation) and 425 nm (emission).
          Chemical formulaC₄₉H₅₅N₉O₇ · HCl
          Peptide SequenceBz-Arg-Gly-Phe-Phe-Pro-4MeOβNA, HCl
          Purity≥95% by HPLC
          SolubilityDMSO (5 mg/ml)
          Storage -20°C
          Protect from moisture
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C.
          Toxicity Standard Handling
          ReferencesTakahashi, T., and Tang, J. 1981. Methods Enzymol. 80, 565.
          Smith, R.E., and Van Frank, R.M. 1975. in Lysosomes in Biology and Pathology 4 (Dingle, J.T., and Dean, R.T., eds.) p. 193, American Elsevier, Holland.