218807 | Caspase-9, Human, Recombinant, E. coli

218807
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      218807-100U
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      Stocked 
      Discontinued
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      Available
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          Plastic ampoule 100 u
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          Description
          OverviewRecombinant, human caspase-9 fused at the N- and C- termini to a His•Tag® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. MW: 11000 and 20000.
          Catalogue Number218807
          Brand Family Calbiochem®
          SynonymsICE-LAP6, Mch6
          References
          ReferencesQin, H., et al. 1999. Nature 399, 549.
          Li, P., et al. 1997. Cell 91, 479.
          Salvesen, G.S., et al. 1997. Cell 91, 443.
          Product Information
          Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1.0 nmol substrate Ac-LEHD-<i>p</i>NA (<a href ="/Products/ProductDisplay.asp→catNO=218805">Cat. No. 218805</a>) per h at 37°C, pH 7.2.
          FormLyophilized
          FormulationLyophilized from 130 mM NaCl, 20 mM Tris, 4 mM (NH₄)₂SO₄
          Applications
          Biological Information
          Purity≥70% by SDS-PAGE
          Specific Activity≥400 units/mg protein
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage ≤ -70°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 4 months at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          218807

          References

          Reference overview
          Qin, H., et al. 1999. Nature 399, 549.
          Li, P., et al. 1997. Cell 91, 479.
          Salvesen, G.S., et al. 1997. Cell 91, 443.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision04-June-2008 RFH
          SynonymsICE-LAP6, Mch6
          DescriptionRecombinant, human caspase-9 fused at the N- and C- termini to a His•Tag® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. MW: 11000 and 20000.
          FormLyophilized
          FormulationLyophilized from 130 mM NaCl, 20 mM Tris, 4 mM (NH₄)₂SO₄
          Recommended reaction conditions50 mM HEPES, 50 mM NaCl, 10 mM DTT, 10 mM EDTA, 5% glycerol, 0.1% CHAPS, pH 7.2. Recommended use: 1 unit per assay.
          Purity≥70% by SDS-PAGE
          Specific activity≥400 units/mg protein
          Unit definitionOne unit is defined as the amount of enzyme that will cleave 1.0 nmol substrate Ac-LEHD-pNA (Cat. No. 218805) per h at 37°C, pH 7.2.
          SolubilityPBS containing 15% glycerol (1 U/µl)
          Storage ≤ -70°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 4 months at -20°C.
          Toxicity Standard Handling
          ReferencesQin, H., et al. 1999. Nature 399, 549.
          Li, P., et al. 1997. Cell 91, 479.
          Salvesen, G.S., et al. 1997. Cell 91, 443.