235423 Caspase-3 Inhibitor I, Cell-Permeable - Calbiochem

235423
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical Formula
      C₉₄H₁₅₈N₂₀O₂₇

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      235423-1MG
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      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
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      Available
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          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          Plastic ampoule 1 mg
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          Description
          OverviewA cell-permeable inhibitor of caspase-3, as well as caspase-6, caspase-7, caspase-8, and caspase-10. The C-terminal DEVD-CHO sequence of this peptide is a highly specific, potent, and reversible inhibitor of caspase-3 (Ki < 1 nM) that has also been shown to strongly inhibit PARP cleavage in cultured human osteosarcoma cell extracts (IC50 = 200 pM). The N-terminal sequence (amino acid residues 1-16) corresponds to the hydrophobic region (h-region) of the signal peptide of Kaposi fibroblast growth factor (K-FGF) and confers cell-permeability to the peptide. A 5 mM (1 mg/100 µl) solution of Caspase-3 Inhibitor I, Cell-permeable (Cat. No. 235427) in DMSO is also available.
          Catalogue Number235423
          Brand Family Calbiochem®
          SynonymsCPP32/Apopain Inhibitor, Cell-permeable, Cell-permeable, DEVD-CHO
          References
          ReferencesThornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
          Lin, Y.-Z., et al. 1996. J. Biol. Chem. 271, 5305.
          Nicholson, D.W. 1996. Nature Biotechnol. 14, 297.
          Schlegel, J., et al. 1996. J. Biol. Chem. 271, 1841.
          Lin, Y.-Z., et al. 1995. J. Biol. Chem. 270, 14255.
          Nicholson, D.W., et al. 1995. Nature 376, 37.
          Tewari, M., et al. 1995. Cell 81, 801.
          Lazebnik, Y.A., et al. 1994. Nature 371, 346.
          Product Information
          ATP CompetitiveN
          FormLyophilized solid
          Hill FormulaC₉₄H₁₅₈N₂₀O₂₇
          Chemical formulaC₉₄H₁₅₈N₂₀O₂₇
          ReversibleY
          Sold on the basis of peptide contentY
          Applications
          Biological Information
          Primary Targetcaspase-3
          Primary Target IC<sub>50</sub>200 pM for inhibiting PARP cleavage in cultured human osteosarcoma cell extracts
          Purity≥97% by HPLC
          Physicochemical Information
          Cell permeableY
          Peptide ContentY
          Peptide SequenceAc-Ala-Ala-Val-Ala-Leu-Leu-Pro-Ala-Val-Leu-Leu-Ala-Leu-Leu-Ala-Pro-Asp-Glu-Val-Asp-CHO
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          235423

          References

          Reference overview
          Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
          Lin, Y.-Z., et al. 1996. J. Biol. Chem. 271, 5305.
          Nicholson, D.W. 1996. Nature Biotechnol. 14, 297.
          Schlegel, J., et al. 1996. J. Biol. Chem. 271, 1841.
          Lin, Y.-Z., et al. 1995. J. Biol. Chem. 270, 14255.
          Nicholson, D.W., et al. 1995. Nature 376, 37.
          Tewari, M., et al. 1995. Cell 81, 801.
          Lazebnik, Y.A., et al. 1994. Nature 371, 346.

          Citations

          Title
        • Jurg Grunenfelder, et al. (2001) Upregulation of Bcl-2 through caspase-3 inhibition ameliorates ischemia/reperfusion injury in rat cardiac allografts. Circulation 104, I202-I206.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision06-February-2014 JSW
          SynonymsCPP32/Apopain Inhibitor, Cell-permeable, Cell-permeable, DEVD-CHO
          DescriptionA cell-permeable inhibitor of caspase-3. The C-terminal DEVD-CHO sequence of this peptide is a highly specific, potent and reversible inhibitor of caspase-3 (Ki <1 nM) that has also been shown to strongly inhibit PARP cleavage in cultured human osteosarcoma cell extracts (IC50 = 0.2 nM). The N-terminal sequence (residues 1-16) corresponds to the hydrophobic region (h-region) of the signal peptide of Kaposi fibroblast growth factor (K-FGF) and confers cell-permeability to the peptide.
          FormLyophilized solid
          Chemical formulaC₉₄H₁₅₈N₂₀O₂₇
          Peptide SequenceAc-Ala-Ala-Val-Ala-Leu-Leu-Pro-Ala-Val-Leu-Leu-Ala-Leu-Leu-Ala-Pro-Asp-Glu-Val-Asp-CHO
          Purity≥97% by HPLC
          SolubilityDMSO (5 mg/ml)
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
          Toxicity Standard Handling
          ReferencesThornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
          Lin, Y.-Z., et al. 1996. J. Biol. Chem. 271, 5305.
          Nicholson, D.W. 1996. Nature Biotechnol. 14, 297.
          Schlegel, J., et al. 1996. J. Biol. Chem. 271, 1841.
          Lin, Y.-Z., et al. 1995. J. Biol. Chem. 270, 14255.
          Nicholson, D.W., et al. 1995. Nature 376, 37.
          Tewari, M., et al. 1995. Cell 81, 801.
          Lazebnik, Y.A., et al. 1994. Nature 371, 346.
          Citation
        • Jurg Grunenfelder, et al. (2001) Upregulation of Bcl-2 through caspase-3 inhibition ameliorates ischemia/reperfusion injury in rat cardiac allografts. Circulation 104, I202-I206.