218709 | Casein Kinase II, α2, Active, Human, Recombinant, E. coli

218709
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      218709-10UG
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          Plastic ampoule 10 μg
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          Description
          OverviewRecombinant, human casein kinase II, α2, (CKII, α2) fused to maltose binding protein (MBP) at the N-terminus and expressed in E. coli. CKII, α2 is a catalytic subunit of the serine/threonine protein kinase CKII. It is suitable for use in reactions where autophosphorylation of the regulatory CKII β-subunit is not desired. Shown to be extremely salt-sensitive with respect to its activity.
          Catalogue Number218709
          Brand Family Calbiochem®
          SynonymsCK2α2, CSNK2A2
          Application Data
          Increasing concentrations of Casein Kinase II, α2 were assayed according to the protocol outlined above.
          References
          ReferencesAntonelli, M., et al. 1997. Eur. J. Biochem. 241, 272.
          Xu, X., et al. 1997. Genomics 48, 79.
          Boldyreff, B., et al. 1994. Cell. Mol. Biol. Res. 40, 391.
          Product Information
          Unit of DefinitionOne unit is defined as the amount of enzyme required to transfer 1 pmol phosphate to RRRADDSDDDDD peptide substrate per min at 30°C.
          FormLiquid
          FormulationIn 50 mM Tris-HCl, 500 mM NaCl, 10 mM DTT, pH 8.5.
          Applications
          Biological Information
          Purity≥ 90% by SDS PAGE
          Specific Activity≥841.819 Units/mg protein
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          218709

          References

          Reference overview
          Antonelli, M., et al. 1997. Eur. J. Biochem. 241, 272.
          Xu, X., et al. 1997. Genomics 48, 79.
          Boldyreff, B., et al. 1994. Cell. Mol. Biol. Res. 40, 391.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision17-April-2017 JSW
          SynonymsCK2α2, CSNK2A2
          Application Data
          Increasing concentrations of Casein Kinase II, α2 were assayed according to the protocol outlined above.
          DescriptionRecombinant, human casein kinase II, α2, (CKII, α2) fused to maltose binding protein (MBP) at the N-terminus and expressed in E. coli. CKII, α2 is a catalytic subunit of the serine/threonine protein kinase CKII. It is suitable for use in reactions where autophosphorylation of the regulatory CKII β-subunit is not desired. Shown to be extremely salt-sensitive with respect to its activity.
          FormLiquid
          FormulationIn 50 mM Tris-HCl, 500 mM NaCl, 10 mM DTT, pH 8.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          Recommended reaction conditions
          Kinase Activity Assay Required Materials •Assay Buffer: 25 mM β-glycerophosphate, 20 mM Tris-HCl, 5 mM EGTA, 1 mM DTT, pH 7.5 •Substrate: 800 µM RRRDDDSDDD peptide substrate •CKII, α2: 5-10 ng/µl CKII, α2 diluted in Assay Buffer just prior to use •Magnesium/ATP Mix: 75 mM MgCl2, 500 µM ATP •Diluted [γ-32P]ATP/Magnesium/ATP Mix: Mix 197 µl Magnesium/ATP Mix with 3 µl (30 µCi) [γ-32P]ATP (3000 Ci/mmol) Protocol 1. Pipette 10 µl Assay Buffer into a clean microcentrifuge tube on ice. 2. Add 10 µl Substrate. 3. Add 10 µl diluted CKII, α2. 4. Add 10 µl Diluted [γ-32P]ATP/Magnesium/ATP Mix. 5. Incubate at 37° for 10 min. 6. Stop the reaction by placing the tubes on ice. 7. Spot 10 µl of each reaction on P81 paper and allow to bind for 30 s. 8. Wash by immersing the paper in 0.75% phosphoric acid and gently shaking. Repeat for a total of 3 washes. 9. Wash as in step 8 using acetone. 10. Dry using an infrared light. 11. Determine the cpm using a scintillation counter or imager.
          Purity≥ 90% by SDS PAGE
          Specific activity≥841.819 Units/mg protein
          Unit definitionOne unit is defined as the amount of enzyme required to transfer 1 pmol phosphate to RRRADDSDDDDD peptide substrate per min at 30°C.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesAntonelli, M., et al. 1997. Eur. J. Biochem. 241, 272.
          Xu, X., et al. 1997. Genomics 48, 79.
          Boldyreff, B., et al. 1994. Cell. Mol. Biol. Res. 40, 391.