208771 | Calpain Substrate III, Fluorogenic - Calbiochem

208771
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      208771-1MG
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          Plastic ampoule 1 mg
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          Description
          OverviewA fluorogenic FRET peptide with a substrate sequence that is optimized for calpain-1 and -2 (Cat. Nos. 208712, 208713, 208715, and 208718) based on 106 known cleavage sites. It is cleaved by mammalian calpains with a much better efficacy (kcat/Kmx10-2 (M-1s-1) = 69.8, 38.5, 20.0, 6.81, 3.58, 0.05, and < 0.05 for calpain-2, trypsin, papain, calpain-B, calpain-A, chymotrypsin, and cathepsin-B, respectively) and kinetically superior to other commonly used calpain substrates, such as LY-AMC (Cat. No. 208731) and α-spectrin cleavage site-based substrate (Cat. No. 208748). Has been used successfully in monitoring calpain activity in whole Drosophila S2 cells (cellular incorporation achieved with a lipofection reagent) and in COS-7 cell lysate.
          Catalogue Number208771
          Brand Family Calbiochem®
          Synonyms(DABCYL)-TPLK~SPPPSPR-(EDANS)
          References
          ReferencesTompa, P., et al. 2004. J. Biol. Chem. 279, 20775.
          Product Information
          ATP CompetitiveN
          FormRed lyophilized solid
          FormulationSupplied as a trifluoroacetate salt.
          Hill FormulaC₈₀H₁₁₄N₂₀O₁₈S
          Chemical formulaC₈₀H₁₁₄N₂₀O₁₈S
          ReversibleN
          Applications
          Biological Information
          Primary TargetSubstrate sequence that is optimized for calpain-1 and -2
          Primary Target IC<sub>50</sub>kcat/Kmx10-2 (M-1s-1) = 69.8, 38.5, 20.0, 6.81, 3.58, 0.05, and < 0.05 for calpain-2, trypsin, papain, calpain-B, calpain-A, chymotrypsin, and cathepsin-B, respectively
          Purity≥98% by HPLC
          Physicochemical Information
          Cell permeableN
          Emission max.
          Excitation max.
          Peptide SequenceDABCYL-Thr-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-EDANS
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Irritant
          Storage -20°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          208771

          References

          Reference overview
          Tompa, P., et al. 2004. J. Biol. Chem. 279, 20775.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision28-May-2008 RFH
          Synonyms(DABCYL)-TPLK~SPPPSPR-(EDANS)
          DescriptionA fluorogenic FRET peptide with a substrate sequence that is optimized for calpain-1 and -2 based on 106 known cleavage sites. It is cleaved by mammalian calpains with higher efficiency (kcat/Kmx10-2 (M-1s-1) = 69.8, 38.5, 20.0, 6.81, 3.58, 0.05, and < 0.05 for calpain-2, trypsin, papain, calpain-B, calpain-A, chymotrypsin, and cathepsin-B, respectively). It is kinetically superior to other commonly used calpain substrates, such as LY-AMC (Cat. No. 208731) and α-spectrin cleavage site-based substrate (Cat. No. 208748). Has been used successfully in monitoring calpain activity in intact Drosophila S2 cells (cellular incorporation achieved with a lipofection reagent) and in COS-7 cell lysate.
          FormRed lyophilized solid
          FormulationSupplied as a trifluoroacetate salt.
          Intert gas (Yes/No) Packaged under inert gas
          Chemical formulaC₈₀H₁₁₄N₂₀O₁₈S
          Peptide SequenceDABCYL-Thr-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-EDANS
          Purity≥98% by HPLC
          SolubilityDMSO (5 mg/ml) or H₂O (1 mg/ml)
          Storage -20°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Toxicity Irritant
          ReferencesTompa, P., et al. 2004. J. Biol. Chem. 279, 20775.