70794 BugBuster® GST•Bind™ Purification Kit

70794
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service
      View Pricing & Availability

      Overview

      Replacement Information

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      70794-3
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          Glass bottle 1 kit
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          Overview

          The BugBuster® GST•Bind™ Purification Kit combines the GST•Bind Resin, GST•Bind Buffer Kit reagents and BugBuster Protein Extraction Reagent for convenient preparation of soluble cell extracts and affinity purification of GST•Tag™ fusion proteins. BugBuster Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E. coli, resulting in the liberation of soluble protein. Cells are harvested by centrifugation as usual, followed by suspension in BugBuster Reagent. During a brief incubation, soluble proteins are released. The extract is clarified by centrifugation, which removes cell debris and insoluble proteins. The clarified extract is ready to apply to GST•Bind Resin.



          Catalogue Number70794
          Brand Family Novagen®
          References
          Product Information
          2 x 100 mlBugBuster Protein Extraction Reagent
          10,000 UBenzonase Nuclease, purity > 90%
          10 mlGST•Bind Resin
          pkg/4Chromatography Columns
          2 x 100 ml10X GST Bind/Wash Buffer
          40 ml10X Glutathione Reconstitution Buffer
          1 gGlutathione, Reduced
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          R PhraseR: 36/38

          Irritating to eyes and skin.
          S PhraseS: 26

          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage Multiple storage requirements
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          BugBuster® GST•Bind™ Purification Kit SDS

          Title

          Safety Data Sheet (SDS) 

          BugBuster® GST•Bind™ Purification Kit Certificates of Analysis

          TitleLot Number
          70794

          Citations

          Title
        • Xiaobing Chen, et al. (2008) Organization of the core structure of the postsynaptic density. Procedings of the National Academy of Science 105, 4453-4458.
        • Bryan T. Carroll, et al. (2006) Sulfated signal from ASJ sensory neurons modulates stomatin-dependent coordination in Caenorhabditis elegans. Journal of Biological Chemistry 281, 35989-35996.
        • Laigeng Li, et al. (2005) Clarification of cinnamoyl co-enzyme a reductase catalysis in monolignol biosynthesis of aspen. Plant and Cell Physiology 46, 1073-1082.
        • User Protocols

          Title
          TB235 GST•Bind™ Kits
          TB245 BugBuster® Protein Extraction Reagent