203900 | Bufalin - CAS 465-21-4 - Calbiochem

203900
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical FormulaCAS #
      C₂₄H₃₄O₄ 465-21-4

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      203900-10MG
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          Alu drum 10 mg
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          Description
          OverviewA cardiotonic steroid isolated from toads of Bufo species that potently inhibits ouabain-sensitive Na+,K+-ATPase activity (IC50 = 1.4 nM). Induces apoptosis in various human cell lines such as leukemia HL-60 and U937 cells by altering the expression of apoptosis-related genes. Reported to stimulate the activities of p21 activated kinase (PAK) and c-Jun Kinase (JNK) in leukemic cell lines. Induces a transient increase in the expression of Tiam1 mRNA in U937 cell lines (~100 nM). Bufalin also down-regulates gene expression of the intracellular signaling protein 14-3-3 in rat lens, probably a consequence of Na+,K+-ATPase inhibition. Reduces the level of topoisomerase II in human leukemia cells.
          Catalogue Number203900
          Brand Family Calbiochem®
          Synonyms5β, 20(22)-Bufadienolide-3β, 14-diol
          References
          ReferencesKurosawa, M., et al. 2000. Am. J. Physiol. 278, C500.
          Kawazoe, N., et al. 1999. Oncogene 18, 2413.
          McGowan, M.H., et al. 1999. J. Pharmacol. Exp. Ther. 289, 1559.
          Watabe, M., et al. 1998. Oncogene 16, 779.
          Hashimoto, S., et al. 1997. Leuk. Res. 21, 875.
          Morris, J.F., et al. 1997. Comp. Biochem. Physiol. A Physiol. 118, 599.
          Brownlee, A.A., et al. 1990. Clin. Sci. 78, 169.
          Product Information
          CAS number465-21-4
          ATP CompetitiveN
          FormWhite to off-white solid
          Hill FormulaC₂₄H₃₄O₄
          Chemical formulaC₂₄H₃₄O₄
          ReversibleN
          Structure formula ImageStructure formula Image
          Applications
          Biological Information
          Primary TargetOuabain-sensitive Na+,K+-ATPase activity
          Primary Target IC<sub>50</sub>1.4 nM against ouabain-sensitive Na+,K+-ATPase activity
          Purity≥98% by HPLC
          Physicochemical Information
          Cell permeableN
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSEI2962500
          Safety Information
          R PhraseR: 26/27/28

          Very toxic by inhalation, in contact with skin and if swallowed.
          S PhraseS: 22-36/37/39-45

          Do not breathe dust.
          Wear suitable protective clothing, gloves and eye/face protection.
          In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Highly Toxic
          Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
          Storage -20°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          203900

          References

          Reference overview
          Kurosawa, M., et al. 2000. Am. J. Physiol. 278, C500.
          Kawazoe, N., et al. 1999. Oncogene 18, 2413.
          McGowan, M.H., et al. 1999. J. Pharmacol. Exp. Ther. 289, 1559.
          Watabe, M., et al. 1998. Oncogene 16, 779.
          Hashimoto, S., et al. 1997. Leuk. Res. 21, 875.
          Morris, J.F., et al. 1997. Comp. Biochem. Physiol. A Physiol. 118, 599.
          Brownlee, A.A., et al. 1990. Clin. Sci. 78, 169.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision22-May-2008 RFH
          Synonyms5β, 20(22)-Bufadienolide-3β, 14-diol
          DescriptionA cardiotonic steroid isolated from Bufo species toads that potently inhibits ouabain-sensitive Na+,K+-ATPase activity. Induces apoptosis in various human cell lines such as leukemia HL60 and U937 cells by altering the expression of apoptosis-related genes. Reported to stimulate the activities of p21 activated kinase (PAK) and c-Jun Kinase (JNK) in leukemic cell lines. Induces a transient increase in the expression of Tiam1 mRNA in U937 cell lines (~100 nM). Bufalin also down-regulates gene expression of the intracellular signaling protein 14-3-3 in rat lens, probably a consequence of Na+-K+-ATPase inhibitors. Reduces the level of topoisomerase II in human leukemia cells.
          FormWhite to off-white solid
          Intert gas (Yes/No) Packaged under inert gas
          CAS number465-21-4
          RTECSEI2962500
          Chemical formulaC₂₄H₃₄O₄
          Structure formulaStructure formula
          Purity≥98% by HPLC
          SolubilityDMSO (25 mg/ml) or warm Ethanol (25 mg/ml)
          Storage -20°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Toxicity Highly Toxic
          ReferencesKurosawa, M., et al. 2000. Am. J. Physiol. 278, C500.
          Kawazoe, N., et al. 1999. Oncogene 18, 2413.
          McGowan, M.H., et al. 1999. J. Pharmacol. Exp. Ther. 289, 1559.
          Watabe, M., et al. 1998. Oncogene 16, 779.
          Hashimoto, S., et al. 1997. Leuk. Res. 21, 875.
          Morris, J.F., et al. 1997. Comp. Biochem. Physiol. A Physiol. 118, 599.
          Brownlee, A.A., et al. 1990. Clin. Sci. 78, 169.