203189 Biotin-X-NHS, Water-Soluble

203189
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      203189-50MG
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          Plastic ampoule 50 mg
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          Description
          OverviewReadily couples biotin to primary amino groups of proteins under mild alkaline conditions. Ideally suited for labeling antibodies, hormones, or other polypeptide molecules containing lysine residues. Extended spacer arm provides maximum accessibility of biotin for avidin conjugates.
          Catalogue Number203189
          Brand Family Calbiochem®
          SynonymsSulfobiotin-X-NHS, Sulfosuccinimidyl-6-(biotinamido)hexanoate
          References
          ReferencesGretch, D.R., et al. 1987. Anal. Biochem. 163, 270.
          Product Information
          FormOff white to tan solid
          Hill FormulaC₂₀H₂₉N₄O₉S₂ · Na
          Chemical formulaC₂₀H₂₉N₄O₉S₂ · Na
          Applications
          Biological Information
          Purity≥94% by HPLC
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Protect from Moisture Protect from moisture
          Do not freeze Ok to freeze
          Special InstructionsUnstable in aqueous solution; reconstitute just prior to use. Following reconstitution in DMSO, Aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 1 month -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          203189

          References

          Reference overview
          Gretch, D.R., et al. 1987. Anal. Biochem. 163, 270.

          Brochure

          Title
          Protein Modifications Tools
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision20-May-2008 RFH
          SynonymsSulfobiotin-X-NHS, Sulfosuccinimidyl-6-(biotinamido)hexanoate
          DescriptionWater solubility is extremely convenient, eliminating the need to dissolve the reagent in an organic solvent such as dimethylformamide (DMF) and dimethylsulfoxide (DMSO) prior to use.
          FormOff white to tan solid
          Recommended reaction conditions
          Buffers containing amines (such as Tris or glycine) should be avoided because they can compete with the reaction. It is also very important that the stock solution be prepared just prior to addition of the reaction mixture, because these compounds are prone to hydrolysis by water. Water-soluble crosslinkers can be dissolved in water or buffer at a concentration of 10-25 mM. Phosphate buffers may precipitate sulfo-N-hydroxysuccinimide (sulfo-NHS) containing compounds. In addition, the solid crosslinker can be added directly to the sample to minimize hydrolysis. Hydrolysis is more pronounced in dilute protein solutions, but can be minimized by using more concentrated protein solution (greater than 5 mg/ml). If you prefer to work with stock solutions, we recommend that you dissolve the crosslinker in anhydrous DMSO at a concentration of 10-25 mM. This solution can then be diluted into the buffer of your choice just prior to use. General Handling 1. Store desiccated at 4°C. 2. Warm contents to room temperature completely before opening. Allow at least 30 min. 3. Wipe the vial to remove any moisture. 4. After opening, store under nitrogen. 5. Protect from moisture.
          Chemical formulaC₂₀H₂₉N₄O₉S₂ · Na
          Purity≥94% by HPLC
          SolubilitydH₂O (5.5 mg/ml) or DMSO (13 mg/ml)
          Storage +2°C to +8°C
          Protect from moisture
          Do Not Freeze Ok to freeze
          Special InstructionsUnstable in aqueous solution; reconstitute just prior to use. Following reconstitution in DMSO, Aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 1 month -20°C.
          Toxicity Standard Handling
          ReferencesGretch, D.R., et al. 1987. Anal. Biochem. 163, 270.