L6113-BC Biocoll

L6113-BC
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      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      L6113
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          100 ml
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          Description
          OverviewBiocoll separating solution does contain a polymer with a molecular weight of approx. 400000 Dalton. Densities of up to 1.1 g/ml can be adjusted using this hydrophilic polymer. For optimal pH and osmolality, adjusting Biocoll with an acid, preferably amidotrizoeic acid(ATA), and sodium hydroxide is required. Biocoll with densities of 1.077 g/ml (cat. no. L 6113/5) and 1.090 g/ml (cat. no. L 6125) are already adjusted accordingly.

          Specifications:

          Sterility: Pass
          Functional Test: Pass
          pH: 6.37-7.57
          Osmolality: 0.285-0.327 Osm/kg H20
          Endotoxin: Pass
          Density: 1.076-1.078 g/ml
          Color: Colorless
          Appearance: Clear

          Separating solutions:

          Separating solutions are used for the isolation of cells or cellular particles during density gradient centrifugation. We offer two different separating solutions: Biocoll and Easycoll. These two solutions differ in the respectively contained polymer. Biocoll separating solution does contain a polymer with a molecular weight of approx. 400000 Dalton. The basis of Easycoll is silica gel particles, coated with PVP (polyvinyl pyrrolidone). The ready-made solutions are already preset on physiological values of osmolality and ph-value.

          Recommendations:

          The ready-to-use separating solution Easycoll is already adjusted to physiological conditions of osmolality and pH value. One can also adjust a required density yourself diluting Easycoll correspondingly.

          Separating lymphocytes using Biocoll:

          1. Biocoll separating solution (D = 1.077 at +20°C) is given to 15 or 25 ml centrifugal tubes in volumes of 7 ml and 10 ml, resp.
          2. Equal parts of heparinized whole blood (50 U/ml heparin, stabilizer-free) and culture medium are mixed and carefully applied over Biocoll separating solution.
          3. Centrifuge 1200xg for 20 min.
          4. Take layer of enriched (70 - 100 %) lymphocytes (between plasma and Biocoll) with a Pasteur′s pipette and wash twice in culture medium:
          • for 10 min at 300xg
          • for 10 min at 200xg
          5. Cell count as usual.
          Catalogue NumberL6113-BC
          Brand Family Calbiochem®
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          Ship Code Ambient Temperature Only
          Storage +2°C to +25°C
          Do not freeze Ok to freeze
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