200275 | Berberine Chloride - CAS 633-65-8 - Calbiochem

200275
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical FormulaCAS #
      C₂₀H₁₈NO₄+Cl- 633-65-8

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      200275-25MG
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          Glass bottle 25 mg
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          Description
          OverviewA highly potent and selective oxysterol EBI2 (GPR183) agonist (Kd) = 450 pM in a saturation binding assay, and EC50 = 140 pM over EC50 = 2.1 nM for its enantiomer, 7β,25-OHC, in a GTP-γS binding assay). Dose-dependently suppresses forskolin-induced cAMP accumulation in an EBI2-expressing SK-N-MC/CRE-β-galactosidase cell line (IC50 = 2 nM), but not in control cells. Stimulates migration of LPS-activated spleen B-cells and anti-CD3/CD28-activated CD4+ T-cells in a dose-dependent manner. In addition, pharmacological inhibition of its biosynthesis in vivo by Clotrimazole, a CYP7B1inhibitor, promotes the migration of adoptively transferred pre-activated B cells to the T/B boundary, mimicking the phenotype of pre-activated B cells in EBI2-deficient mice.
          Catalogue Number200275
          Brand Family Calbiochem®
          SynonymsBerberine
          References
          ReferencesKinm. B., et al. 2011, Brit. Journ. Pharm.164, 106.
          Kong, W., et al. 2004, Nature Med. 10, 1344.
          Dimlich, R. et al. 1980, Stain Technol. 55, 217.
          Product Information
          CAS number633-65-8
          FormYellow powder
          Hill FormulaC₂₀H₁₈NO₄+Cl-
          Chemical formulaC₂₀H₁₈NO₄+Cl-
          Structure formula ImageStructure formula Image
          Applications
          Biological Information
          Purity≥95% by HPLC (NMR if no UV signal)
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          200275

          References

          Reference overview
          Kinm. B., et al. 2011, Brit. Journ. Pharm.164, 106.
          Kong, W., et al. 2004, Nature Med. 10, 1344.
          Dimlich, R. et al. 1980, Stain Technol. 55, 217.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision26-April-2012 JSW
          SynonymsBerberine
          DescriptionA highly potent and selective oxysterol EBI2 (GPR183) agonist (Kd) = 450 pM in a saturation binding assay, and EC50 = 140 pM over EC50 = 2.1 nM for its enantiomer, 7β,25-OHC, in a GTP-γS binding assay). Dose-dependently suppresses forskolin-induced cAMP accumulation in an EBI2-expressing SK-N-MC/CRE-β-galactosidase cell line (IC50 = 2 nM), but not in control cells. Stimulates migration of LPS-activated spleen B-cells and anti-CD3/CD28-activated CD4+ T-cells in a dose-dependent manner. In addition, pharmacological inhibition of its biosynthesis in vivo by Clotrimazole, a CYP7B1inhibitor, promotes the migration of adoptively transferred pre-activated B cells to the T/B boundary, mimicking the phenotype of pre-activated B cells in EBI2-deficient mice.
          FormYellow powder
          Intert gas (Yes/No) Packaged under inert gas
          CAS number633-65-8
          Chemical formulaC₂₀H₁₈NO₄+Cl-
          Structure formulaStructure formula
          Purity≥95% by HPLC (NMR if no UV signal)
          SolubilityDMSO (10 mg/ml) or H₂O (1 mg/ml)
          Storage +2°C to +8°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Toxicity Standard Handling
          ReferencesKinm. B., et al. 2011, Brit. Journ. Pharm.164, 106.
          Kong, W., et al. 2004, Nature Med. 10, 1344.
          Dimlich, R. et al. 1980, Stain Technol. 55, 217.