72157 BacMagic™-2 DNA Kit - Novagen

72157
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      Overview

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      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      72157-3
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          Plastic ampoule 5 rxn
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          Description
          OverviewThe BacMagic™-2 DNA Kit provides a positive selection for creating baculoviruses recombinants in preparation for generating recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification. Like BacMagic™ DNA, BacMagic-2 DNA is derived from an AcNPV genome, contains an ORF 1629 deletion which prevents nonrecombinant virus from replicating in insect cells, and contains a deletion of the nonessential chitinase (chiA) gene, to improve production of secreted and membrane-targeted proteins. BacMagic-2 DNA have an additional deletion of the v-cath gene, a cathespsin-like cysteine protease, resulting in significantly improved quality and yield for most target proteins. The BacMagic-2 DNA Kit contains the BacMagic-2 DNA, Insect GeneJuice® Transfection Reagent, and a positive control plasmid to monitor co-transfection efficiency.
          Not available for sale in Japan
          Catalogue Number72157
          Brand Family Novagen®
          Application Data
          Figure 1. Construction of baculovirus recombinants with three BacMagic systems

          Figure 2. Streamlined baculovirus method
          References
          Product Information
          0.5 µgBacMagic-2 DNA
          50 µlInsect GeneJuice Transfection Reagent
          2 µgTransfection Control Plasmid
          DeclarationNot available for sale in Japan.
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          S PhraseS: 24/25

          Avoid contact with skin and eyes.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          BacMagic™-2 DNA Kit - Novagen Certificates of Analysis

          TitleLot Number
          72157

          User Protocols

          Title
          TB459 BacMagic™ DNA Kits