216254 | BCECF/AM - CAS 117464-70-7 - Calbiochem

216254
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      Overview

      Replacement Information

      Key Specifications Table

      CAS #
      117464-70-7

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      216254-1MG
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          Glass bottle 1 mg
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          Description
          OverviewA variable mixture of cell-permeable ester derivatives of BCECF that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF. Has been used to monitor intracellular pH changes in mammalian fibroblasts, gastric cells, lymphocytes, myocytes, and distal convoluted tubules.
          Catalogue Number216254
          Brand Family Calbiochem®
          Synonyms2ʹ,7ʹ-bis-(Carboxyethyl)-5(6ʹ)-carboxyfluorescein Acetoxymethyl Ester
          References
          ReferencesFutsaetner, C.M., et al. 1993. Can. J. Microbiol. 39, 180.
          Hayashi, H., et al. 1992. Am. J. Physiol. 262, C628.
          Sasaki, S., et al. 1992. Biochim. Biophys. Acta 1137, 45.
          Miyata, H., et al. 1989. Biochem. Biophys. Res. Commun. 163, 500.
          Weiner, I.D., and Hamm, L.L. 1989. Am. J. Physiol. 256, F957.
          Product Information
          CAS number117464-70-7
          ATP CompetitiveN
          FormWhite to off-white solid
          ReversibleN
          Applications
          Biological Information
          Primary TargetMonitor intracellular pH changes
          Purity≥93% by HPLC (mixture of isomers)
          Physicochemical Information
          Cell permeableY
          Emission max.
          Excitation max.
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          R PhraseR: 36/37/38

          Irritating to eyes, respiratory system and skin.
          S PhraseS: 36-22-26

          Wear suitable protective clothing.
          Do not breathe dust.
          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Irritant
          Storage -20°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 week at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          216254

          References

          Reference overview
          Futsaetner, C.M., et al. 1993. Can. J. Microbiol. 39, 180.
          Hayashi, H., et al. 1992. Am. J. Physiol. 262, C628.
          Sasaki, S., et al. 1992. Biochim. Biophys. Acta 1137, 45.
          Miyata, H., et al. 1989. Biochem. Biophys. Res. Commun. 163, 500.
          Weiner, I.D., and Hamm, L.L. 1989. Am. J. Physiol. 256, F957.

          Brochure

          Title
          Dyes and Stains Technical Bulletin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision02-June-2014 JSW
          Synonyms2ʹ,7ʹ-bis-(Carboxyethyl)-5(6ʹ)-carboxyfluorescein Acetoxymethyl Ester
          DescriptionA variable mixture of cell-permeable ester derivatives of BCECF (Cat. No. 216252) that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF. Has been used to monitor intracellular pH changes in mammalian fibroblasts, gastric cells, lymphocytes, myocytes, and distal convoluted tubules. Typical loading buffer contains 1-10 µM BCECF/AM in serum-free medium that is free of amino acids and buffers containing primary or secondary amines.
          FormWhite to off-white solid
          Intert gas (Yes/No) Packaged under inert gas
          CAS number117464-70-7
          Purity≥93% by HPLC (mixture of isomers)
          SolubilityDMSO (25 mg/ml) or DMF
          Storage -20°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 week at -20°C.
          Toxicity Irritant
          ReferencesFutsaetner, C.M., et al. 1993. Can. J. Microbiol. 39, 180.
          Hayashi, H., et al. 1992. Am. J. Physiol. 262, C628.
          Sasaki, S., et al. 1992. Biochim. Biophys. Acta 1137, 45.
          Miyata, H., et al. 1989. Biochem. Biophys. Res. Commun. 163, 500.
          Weiner, I.D., and Hamm, L.L. 1989. Am. J. Physiol. 256, F957.