71285 BCA Protein Assay Kit

71285
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      Overview

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      71285-3
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          Glass bottle 500 assa
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          Description
          OverviewThe BCA protein assay is based on a biuret reaction, which is the reduction of Cu2+ to Cu+ by proteins in an alkaline solution with concentration-dependent detection of the monovalent copper ions. Bicinchoninic acid is a chromogenic reagent that chelates the reduced copper, producing a purple complex with strong absorbance at 562 nm (Smith 1985, Wiechelman 1988). This assay can be used to quantify protein concentration with a wide variety of samples and can be performed in minutes.

          The Novagen® BCA Protein Assay Kit can be used to determine protein concentration in the range of 20-2000 µg/ml in either a standard assay or microassay configuration. Kit components are sufficient to complete 500 standard-size reactions (50 µl protein sample plus 1 ml reagent) or 2500 micro-scale reactions (25 µl protein sample plus 200 µl reagent). A BSA standard (2 mg/ml) is provided for convenient and reliable preparation of standard curves.

          This assay is robust and can be performed in the presence of many compounds. Some reagents, including chelating agents, strong acids or bases, and reducing agents, interfere with the reduction and chelating reactions on which this assay depends (Brown 1989). The BCA assay is compatible with the following Novagen protein extraction and lysis reagents: BugBuster® Protein Extraction Reagent, PopCulture® Reagent, CytoBuster™ Protein Extraction Reagent, Reportasol™ Extraction Buffer, and Insect PopCulture Reagent. Options for the removal or dilution of interfering substances are described in the kit literature.
          Catalogue Number71285
          Brand Family Novagen®
          References
          ReferencesSmith, P.K., et al. 1985. Anal. Biochem. 150, 76.

          Kessler, R. J. and Fanestil, D. D. 1986. Anal. Biochem 159, 138.

          Wiechelman, K., et al. 1988. Anal. Biochem. 175, 231.

          Brown, R., et al. 1989. Anal. Biochem. 180, 136.
          Product Information
          500 mlBCA Solution
          15 ml4% Cupric Sulfate
          3 x 1 mlBSA Standard, 2 mg/ml
          DeclarationUse of the BCA protein assay is permitted for research purposes only.
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage +15°C to +30°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          BCA Protein Assay Kit SDS

          Title

          Safety Data Sheet (SDS) 

          BCA Protein Assay Kit Certificates of Analysis

          TitleLot Number
          71285

          References

          Reference overview
          Smith, P.K., et al. 1985. Anal. Biochem. 150, 76.

          Kessler, R. J. and Fanestil, D. D. 1986. Anal. Biochem 159, 138.

          Wiechelman, K., et al. 1988. Anal. Biochem. 175, 231.

          Brown, R., et al. 1989. Anal. Biochem. 180, 136.

          Citations

          Title
        • Matthias E. Liechti, et al. (2007) Metabotropic glutamate 2/3 receptors in the ventral tegmental area and the nucleus accumbens shell are involved in behaviors relating to nicotine dependence. Journal of Neuroscience 27, 9077-9085.
        • User Protocols

          Title
          TB380 BCA Protein Assay Kit