ABC118 Anti-phospho Beclin Antibody (Thr119)

ABC118
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, PIA Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberABC118
      DescriptionAnti-phospho Beclin Antibody (Thr119)
      Alternate Names
      • Beclin-1
      • Coiled-coil myosin-like BCL2-interacting protein
      • Protein GT197
      Background InformationThe Beclin-1 (BECN1) gene encodes a 60 kDa coiled-coil protein that interacts with the prototypic apoptosis inhibitor Bcl-2. Beclin-1 promotes autophagy in autophagy-defective yeast with a targeted disruption of agp6/vps30, and in human MCF7 breast carcinoma cells. The autophagy-promoting activity of Beclin-1 in MCF7 cells is associated with inhibition of MCF7 cellular proliferation, in vitro clonigenicity, tumorigenesis, and has been found to ameliorate the neurodegenerative pathology in α-Synuclein models of Parkinson's and lewy body diseases. Endogenous Beclin-1 protein expression is frequently low in human breast epithelial carcinoma cell lines and tissue, but is expressed ubiquitously at high levels in normal breast epithelia. It has also been shown that Beclin 1 contains a leucine-rich nuclear export signal motif raising the possibility that its autophagy and/or tumor suppressor function may require regulated, CRM1-dependent, nucleocytoplasmic transport.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Human prostate tumor tissue lysate.
      PresentationPurified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationDetect Beclin using this rabbit polyclonal antibody, Anti-phospho Beclin Antibody (Thr119) validated for use in western blotting & Peptide Inhibition Assay.
      Key Applications
      • Western Blotting
      • Peptide Inhibition Assay
      Application NotesWestern Blotting/Peptide Inhibition Analysis: 5 µg/mL from a representative lot detected phospho Beclin (Thr119) in LNCap cell lysate. Peptide inhibition was observed with the addition of phospho peptides, but not with the addition of non-phospho peptides.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the BH3 domain of human Beclin phosphorylated at Thr119.
      EpitopeBH3 domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes the BH3 domain of Beclin phosphorylated at Thr119.
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Mouse, Rat, Bovine, Chicken, Porcine, Xenopus based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • BECN1
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~50 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human prostate tumor tissue lysate.

      Western Blotting Analysis: 5 µg/mL of this antibody detected 10 µg of human prostate tumor tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-phospho Beclin Antibody (Thr119) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho Beclin Antibody (Thr119) Certificates of Analysis

      TitleLot Number
      Anti-phospho Beclin (Thr119) - 28361782836178
      Anti-phospho Beclin (Thr119) - 31804453180445
      Anti-phospho Beclin (Thr119) - Q2196317Q2196317
      Anti-phospho Beclin (Thr119) -25275872527587
      Anti-phospho Beclin (Thr119) -27422572742257
      Anti-phospho Beclin (Thr119) -27458992745899
      Anti-phospho Beclin (Thr119) -27830722783072
      Anti-phospho Beclin (Thr119) Polyclonal Antibody2970689
      Anti-phospho Beclin (Thr119) Polyclonal Antibody2890936

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies