OP104L Anti-p53 (Ab-11) (Pantropic) Mouse mAb (PAb1802)

OP104L
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      OP104L-100UG
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      Limited AvailabilityLimited Availability
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      Discontinued
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      Available
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          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes the ~53 kDa wild-type and mutant p53 protein in immunoblotting. Recognizes only the wild-type p53 protein in immunoprecipitation.
          Catalogue NumberOP104L
          Brand Family Calbiochem®
          References
          ReferencesLevine, A.J. 1997. Cell 88, 323.
          Miller, S.D., et al. 1997. Mol. Cell. Biol. 17, 2194.
          Pellegata, N.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 15209.
          Sturzbecher, H.-W., et al. 1996. EMBO J. 15, 1992.
          Argarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8493.
          Götz, C. and Montenarh, M. 1995. Int. J. Oncology 6, 1129.
          Reed, M. et al. 1995. Proc. Natl. Acad. Sci. USA 92, 9455.
          Waldman, T., et al. 1995. Cancer Res. 55, 5187.
          Clarke, A.R., et al. 1994. Oncogene 9, 1767.
          Cho, Y., et al. 1994. Science 265, 346.
          El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
          Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
          Lane, D.P. 1992. Nature 358, 15.
          Product Information
          FormLyophilized
          FormulationLyophilized from a volatile buffer, 100 µg BSA.
          Negative controlSK-OV-3 cells
          Positive controlA431 or SK-BR-3 cells
          PreservativeNone
          Applications
          Application ReferencesImmunoblotting, Immunoprecipitation Banks, L., et al. 1986. Eur. J. Biochem. 159, 529.
          Key Applications Not Immunofluorescence
          Not Paraffin Sections
          Immunoblotting (Western Blotting)
          Immunoprecipitation
          Not Frozen Sections
          Application NotesImmunoblotting (2.5 µg/ml, chemiluminescence, see application references)
          Immunoprecipitation (1 µg/reaction, see comments, see application references)
          Frozen Sections (not recommended)
          Immunofluorescence (not recommended)
          Paraffin Sections (not recommended)
          Application CommentsThis antibody will immunoprecipitate wild-type p53 only. This antibody will stain methanol-fixed cells. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenrecombinant, human p53 protein
          ImmunogenHuman
          Epitopewithin amino acids 306-393 of human p53
          ClonePAb1802
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          • Mouse
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included. Lyophilized antibodies should be reconstituted at 4°C by gradual addition of solvent over a period of 1-2 h with occasional gentle mixing.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-p53 (Ab-11) (Pantropic) Mouse mAb (PAb1802) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-p53 (Ab-11) (Pantropic) Mouse mAb (PAb1802) Certificates of Analysis

          TitleLot Number
          OP104L

          References

          Reference overview
          Levine, A.J. 1997. Cell 88, 323.
          Miller, S.D., et al. 1997. Mol. Cell. Biol. 17, 2194.
          Pellegata, N.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 15209.
          Sturzbecher, H.-W., et al. 1996. EMBO J. 15, 1992.
          Argarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8493.
          Götz, C. and Montenarh, M. 1995. Int. J. Oncology 6, 1129.
          Reed, M. et al. 1995. Proc. Natl. Acad. Sci. USA 92, 9455.
          Waldman, T., et al. 1995. Cancer Res. 55, 5187.
          Clarke, A.R., et al. 1994. Oncogene 9, 1767.
          Cho, Y., et al. 1994. Science 265, 346.
          El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
          Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
          Lane, D.P. 1992. Nature 358, 15.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision01-October-2007 RFH
          ApplicationImmunoblotting (2.5 µg/ml, chemiluminescence, see application references)
          Immunoprecipitation (1 µg/reaction, see comments, see application references)
          Frozen Sections (not recommended)
          Immunofluorescence (not recommended)
          Paraffin Sections (not recommended)
          DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~53 kDa wild-type and mutant forms of p53.
          Backgroundp53 (393 amino acids) exhibits sequence-specific DNA binding, directly interacts with various cellular and viral proteins, and induces cell cycle arrest in response to DNA damage by inducing GADD45, WAF1, and MDM2. p53 is the most commonly mutated gene in human cancer with most mutations occurring in the DNA contact residues. p53 binds damaged DNA non-specifically via its C-terminal 75 amino acids which in turn may be responsible for subsequent activation, sequence-specific DNA binding, and WAF1 induction allowing DNA repair to take place. p53 down regulates bcl-2 expression and up regulates bax expression, but may not always be necessary for apoptosis. In addition to the afore mentioned proteins, two other proteins involved in replication and recombination, RPA and RAD51, also bind p53.
          HostMouse
          Immunogen speciesHuman
          Immunogenrecombinant, human p53 protein
          Epitopewithin amino acids 306-393 of human p53
          ClonePAb1802
          IsotypeIgG₁
          Specieshuman, mouse
          Positive controlA431 or SK-BR-3 cells
          Negative controlSK-OV-3 cells
          FormLyophilized
          FormulationLyophilized from a volatile buffer, 100 µg BSA.
          PreservativeNone
          CommentsThis antibody will immunoprecipitate wild-type p53 only. This antibody will stain methanol-fixed cells. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included. Lyophilized antibodies should be reconstituted at 4°C by gradual addition of solvent over a period of 1-2 h with occasional gentle mixing.
          Toxicity Standard Handling
          ReferencesLevine, A.J. 1997. Cell 88, 323.
          Miller, S.D., et al. 1997. Mol. Cell. Biol. 17, 2194.
          Pellegata, N.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 15209.
          Sturzbecher, H.-W., et al. 1996. EMBO J. 15, 1992.
          Argarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8493.
          Götz, C. and Montenarh, M. 1995. Int. J. Oncology 6, 1129.
          Reed, M. et al. 1995. Proc. Natl. Acad. Sci. USA 92, 9455.
          Waldman, T., et al. 1995. Cancer Res. 55, 5187.
          Clarke, A.R., et al. 1994. Oncogene 9, 1767.
          Cho, Y., et al. 1994. Science 265, 346.
          El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
          Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
          Lane, D.P. 1992. Nature 358, 15.
          Application referencesImmunoblotting, Immunoprecipitation Banks, L., et al. 1986. Eur. J. Biochem. 159, 529.