OP24 Anti-c-K-Ras (Ab-1) Mouse mAb (234-4.2)

OP24
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      OP24-100UG
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      Discontinued
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          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes c-K-Ras and v-K-Ras in SW480 and Y1 cells and normal skin tissue. Cross-reacts with c-H-Ras and c-N-Ras.
          Catalogue NumberOP24
          Brand Family Calbiochem®
          References
          ReferencesMizuuchi, H. et al. 1992. Cancer Res. 2, 2777.
          Rodenhuis, S. et al. 1992. Cancer Res. (Suppl) 52, 2665s.
          Sirdransky, D., et al. 1992. Science 256, 102.
          Shaw, P., et al. 1991. Oncogene 6, 2121.
          Bos, J.L. 1989. Cancer Res. 49, 4682.
          Furth, M.E., et al. 1987. Oncogene 1, 47.
          Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
          Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
          Taparowsky, E., et al. 1983. Cell 34, 581.
          Ellis, R.W., et al. 1981. Nature 292, 506.
          Shih, T.Y., et al. 1980. Nature 287, 686.
          Product Information
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
          Positive controlSW480 or Y1 cells or normal skin tissue
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesImmunoblotting Palejwala, S. and Goldsmith, L.T. 1991. BioTechniques 11, 606.
          Key Applications Paraffin Sections
          Frozen Sections
          Immunoblotting (Western Blotting)
          Immunoprecipitation
          Application NotesFrozen Sections (10 µg/ml)
          Immunoblotting (5 µg/ml with 1% gelatin, see comments and application references)
          Immunoprecipitation (see comments)
          Paraffin Sections (10 µg/ml, saponin or pepsin pre-treatment required)
          Application CommentsThis antibody reacts with c-K-Ras and v-K-Ras, and cross-reacts with c-H-Ras or c-N- Ras by immunoblotting. The level of expression of p21 Ras is variable in different tissues. For this reason we recommend a sample concentration step prior to immunoblotting (see application references). For immunoprecipitation, use 5 µg Cat. No. OP24 per sample with 45 µl protein A agarose. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenrecombinant, rat K-Ras
          ImmunogenRat
          Epitopewithin amino acids 54-189
          Clone234-4.2
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-c-K-Ras (Ab-1) Mouse mAb (234-4.2) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-c-K-Ras (Ab-1) Mouse mAb (234-4.2) Certificates of Analysis

          TitleLot Number
          OP24

          References

          Reference overview
          Mizuuchi, H. et al. 1992. Cancer Res. 2, 2777.
          Rodenhuis, S. et al. 1992. Cancer Res. (Suppl) 52, 2665s.
          Sirdransky, D., et al. 1992. Science 256, 102.
          Shaw, P., et al. 1991. Oncogene 6, 2121.
          Bos, J.L. 1989. Cancer Res. 49, 4682.
          Furth, M.E., et al. 1987. Oncogene 1, 47.
          Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
          Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
          Taparowsky, E., et al. 1983. Cell 34, 581.
          Ellis, R.W., et al. 1981. Nature 292, 506.
          Shih, T.Y., et al. 1980. Nature 287, 686.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision03-June-2009 JSW
          ApplicationFrozen Sections (10 µg/ml)
          Immunoblotting (5 µg/ml with 1% gelatin, see comments and application references)
          Immunoprecipitation (see comments)
          Paraffin Sections (10 µg/ml, saponin or pepsin pre-treatment required)
          DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Ag8.653 mouse myeloma cells. Recognizes the ~21 kDa c-K-Ras and v-K-Ras proteins. Also recognizes c-H-Ras and c-N-Ras.
          BackgroundThe human ras gene family consists of three identified members, H, K and N-ras, encoding proteins of 188-189 amino acids and 21,000 (p21) molecular weight. Human H- and K-ras are the homologues of v-H- and v-K-ras sequences originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. Normal human cellular ras genes can be activated to oncogenes by mutations occurring in codons 12, 13 and 61; such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of Ras proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21s and their relevance to human disease diagnosis and prognosis is still emerging. K-ras mutations have been identified in several cancers to date including lung and colorectal tumors.
          HostMouse
          Immunogen speciesRat
          Immunogenrecombinant, rat K-Ras
          Epitopewithin amino acids 54-189
          Clone234-4.2
          IsotypeIgG2a
          Specieshuman, mouse, rat
          Positive controlSW480 or Y1 cells or normal skin tissue
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsThis antibody reacts with c-K-Ras and v-K-Ras, and cross-reacts with c-H-Ras or c-N- Ras by immunoblotting. The level of expression of p21 Ras is variable in different tissues. For this reason we recommend a sample concentration step prior to immunoblotting (see application references). For immunoprecipitation, use 5 µg Cat. No. OP24 per sample with 45 µl protein A agarose. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesMizuuchi, H. et al. 1992. Cancer Res. 2, 2777.
          Rodenhuis, S. et al. 1992. Cancer Res. (Suppl) 52, 2665s.
          Sirdransky, D., et al. 1992. Science 256, 102.
          Shaw, P., et al. 1991. Oncogene 6, 2121.
          Bos, J.L. 1989. Cancer Res. 49, 4682.
          Furth, M.E., et al. 1987. Oncogene 1, 47.
          Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
          Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
          Taparowsky, E., et al. 1983. Cell 34, 581.
          Ellis, R.W., et al. 1981. Nature 292, 506.
          Shih, T.Y., et al. 1980. Nature 287, 686.
          Application referencesImmunoblotting Palejwala, S. and Goldsmith, L.T. 1991. BioTechniques 11, 606.