OP119 Anti-c-ErbB3 (Ab-4) Mouse mAb (H3.90.6 or Ab90)

OP119
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      OP119-100UG
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      Stocked 
      Discontinued
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      Available
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          Will advise
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          Contact Customer Service

          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes the ~185 kDa c-ErbB3/HER3 protein in T47D cells and breast carcinoma tissue.
          Catalogue NumberOP119
          Brand Family Calbiochem®
          SynonymsAnti-HER3, Anti-ErbB3, Anti-Erythroblastosis Virus
          References
          ReferencesChen, X., et al. 1996. J. Biol. Chem. 271, 7620.
          Carraway, K.L., III, et al. 1994. J. Biol. Chem. 269, 14303.
          Ciardiello, F., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 7792.
          Plowman, G.D., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 4905.
          Kraus, J.H., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 9193.
          Shoyab, M., et al. 1989. Science 243, 1074.
          Kawakami, T., et al. 1986. Mol. Cell. Biol. 6, 4195.
          Product Information
          FormLiquid
          FormulationIn 10 mM PBS, pH 7.4.
          Positive controlT-47D cells or breast or prostate carcinoma tissue
          PreservativeNone
          Applications
          Key Applications Not Western Blot
          Not Neutralization Studies
          Flow Cytometry
          Immunofluorescence
          Immunoprecipitation
          Application NotesFlow Cytometry (see comments)
          Immunoblotting (not recommended)
          Immunofluorescence (see comments)
          Immunoprecipitation (10 µl/mg protein lysate)
          Neutralization Studies (not recommended)
          Application CommentsDoes not cross-react with c-erbB1 (EGFR), c-erbB2/HER2/neu or c-erbB4/HER4. It does not block the binding of heregulin to c-erbB3, has no effect on tyrosine kinase activity, receptor internalization or tumorigenic growth of cells overexpressing c-erbB3. This antibody is particularly well suited for immunoprecipitating the unoccupied as well as the occupied receptor. This antibody has also been reported to work for flow cytometry and immunofluorescence. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenrecombinant, human ErbB3 extracellular domain
          ImmunogenHuman
          CloneH3.90.6 or Ab90
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-c-ErbB3 (Ab-4) Mouse mAb (H3.90.6 or Ab90) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-c-ErbB3 (Ab-4) Mouse mAb (H3.90.6 or Ab90) Certificates of Analysis

          TitleLot Number
          OP119

          References

          Reference overview
          Chen, X., et al. 1996. J. Biol. Chem. 271, 7620.
          Carraway, K.L., III, et al. 1994. J. Biol. Chem. 269, 14303.
          Ciardiello, F., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 7792.
          Plowman, G.D., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 4905.
          Kraus, J.H., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 9193.
          Shoyab, M., et al. 1989. Science 243, 1074.
          Kawakami, T., et al. 1986. Mol. Cell. Biol. 6, 4195.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision03-Febuary-2009 RFH
          SynonymsAnti-HER3, Anti-ErbB3, Anti-Erythroblastosis Virus
          ApplicationFlow Cytometry (see comments)
          Immunoblotting (not recommended)
          Immunofluorescence (see comments)
          Immunoprecipitation (10 µl/mg protein lysate)
          Neutralization Studies (not recommended)
          DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with specified immunogen and fusing splenocytes with NSO mouse myeloma cells. Recognizes the ~185 kDa c-ErbB3 protein.
          BackgroundErbB3/HER3, a member of the epidermal growth factor receptor (EGF-R) family, was originally isolated as a cDNA clone from a human carcinoma cell line. The molecule is transcribed from a 6-kilobase mRNA, which can be identified in a variety of different tissues. Epidermal growth factor (EGF) initiates its effects on cell growth through interaction with a 170,000 dalton cell surface glycoprotein receptor. Binding of EGF, amphiregulin, or transforming growth factor-alpha (TGF-α) to the receptor or to other members of the receptor family activates a tyrosine-specific protein kinase intrinsic to the EGF-receptor protein family. While there is a large degree of conserved homology between the cytoplasmic regions of EGF-R and HER2/neu, the HER3 molecule is more unique. This observation suggests that HER3 is subject to regulatory signals distinct from those affecting EGF-R and HER2/neu and, therefore, signals an alternate biochemical response. In particular, it has been shown that the ligand for HER3 is NDF/heregulin a property shared with HER4. c-ErbB3/HER3 is overexpressed in a variety of tumors (e.g., breast, stomach, pancreas and colon).
          HostMouse
          Immunogen speciesHuman
          Immunogenrecombinant, human ErbB3 extracellular domain
          CloneH3.90.6 or Ab90
          IsotypeIgG2a
          Specieshuman
          Positive controlT-47D cells or breast or prostate carcinoma tissue
          FormLiquid
          FormulationIn 10 mM PBS, pH 7.4.
          Concentration Label Please refer to vial label for lot-specific concentration
          PreservativeNone
          CommentsDoes not cross-react with c-erbB1 (EGFR), c-erbB2/HER2/neu or c-erbB4/HER4. It does not block the binding of heregulin to c-erbB3, has no effect on tyrosine kinase activity, receptor internalization or tumorigenic growth of cells overexpressing c-erbB3. This antibody is particularly well suited for immunoprecipitating the unoccupied as well as the occupied receptor. This antibody has also been reported to work for flow cytometry and immunofluorescence. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesChen, X., et al. 1996. J. Biol. Chem. 271, 7620.
          Carraway, K.L., III, et al. 1994. J. Biol. Chem. 269, 14303.
          Ciardiello, F., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 7792.
          Plowman, G.D., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 4905.
          Kraus, J.H., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 9193.
          Shoyab, M., et al. 1989. Science 243, 1074.
          Kawakami, T., et al. 1986. Mol. Cell. Biol. 6, 4195.