PC234L Anti-Vasopressin Rabbit pAb

PC234L
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      PC234L-100UL
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      Stocked 
      Discontinued
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      Available
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          Plastic ampoule 100 ul
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          Description
          OverviewRecognizes vasopressin in rat hypothalamus.
          Catalogue NumberPC234L
          Brand Family Calbiochem®
          Application Data
          Detection of rat vasopressin by immunohistochemistry. Sample: Rat paraventricular nucleus and supraoptic nucleus. Primary antibody: Anti-Vasopressin Rabbit pAb (Cat. No. PC234L) (1:400). Detection: fluorescence.
          References
          ReferencesSudhof, T.C. 1995. Nature 375, 645.
          Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.
          Product Information
          FormLyophilized
          FormulationUndiluted serum.
          Positive controlRat hypothalamus
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Frozen Sections
          Immunofluorescence
          Application NotesFrozen Sections (1:400-1:800, Cy3; 1:2000-1:4000, Bn-SA/HRP)
          Immunofluorescence (1:200-1:400)
          Application CommentsAntibody specificity was examined in the rat paraventricular nucleus and supraoptic nucleus. Staining is completely eliminated by pretreatment of antibody with arginine vasopressin (10 µg/ml). Preabsorption of antibody with oxytocin (100 µg/ml) resulted in no loss of immunostaining. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenrat arginine vasopressin
          ImmunogenRat
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months. Avoid freeze/thaw cycles of solutions.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-Vasopressin Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-Vasopressin Rabbit pAb Certificates of Analysis

          TitleLot Number
          PC234L

          References

          Reference overview
          Sudhof, T.C. 1995. Nature 375, 645.
          Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision20-April-2009 JSW
          ApplicationFrozen Sections (1:400-1:800, Cy3; 1:2000-1:4000, Bn-SA/HRP)
          Immunofluorescence (1:200-1:400)
          Application Data
          Detection of rat vasopressin by immunohistochemistry. Sample: Rat paraventricular nucleus and supraoptic nucleus. Primary antibody: Anti-Vasopressin Rabbit pAb (Cat. No. PC234L) (1:400). Detection: fluorescence.
          DescriptionRabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes vasopressin.
          BackgroundNeurotransmitters can be defined as chemical substances that are released by the presynaptic neuron before being bound by receptors on the postsynaptic cell surface. It has been well established that the nervous system utilizes neurotransmitters for cell signaling. Neurotransmitters can be divided into two groups; low molecular weight transmitter molecules such as biogenic amines, purines, or amino acids, and bioactive peptides, of which more than 50 members have been identified in the mammalian central nervous system. Many low molecular weight neurotransmitters are produced within the axon terminal from common cellular metabolites and packaged into synaptic vesicles. In contrast, neuropeptides are synthesized in the cell body and packaged into large dense core vesicles for delivery to the presynaptic membrane. The transport and delivery of vesicles containing neurotransmitter is a highly regulated and complex process. The neuropeptide vasopressin plays important roles in vasoconstriction, antinociception, behavior, electrolyte balance, and coagulation functions. Vasopressin helps regulate the hypothalamic-pituitary-adrenal axis along with the corticotropin-releasing hormone. The immuno-localization, neuronal distribution, and vesicular compartmentalization of neurotransmitter molecules and their precursors are important for understanding the modulation, regulation, and function of neurotransmitters in the nervous system.
          HostRabbit
          Immunogen speciesRat
          Immunogenrat arginine vasopressin
          IsotypeIgG
          Speciesrat
          Positive controlRat hypothalamus
          FormLyophilized
          FormulationUndiluted serum.
          Preservative≤0.1% sodium azide
          CommentsAntibody specificity was examined in the rat paraventricular nucleus and supraoptic nucleus. Staining is completely eliminated by pretreatment of antibody with arginine vasopressin (10 µg/ml). Preabsorption of antibody with oxytocin (100 µg/ml) resulted in no loss of immunostaining. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months. Avoid freeze/thaw cycles of solutions.
          Toxicity Standard Handling
          ReferencesSudhof, T.C. 1995. Nature 375, 645.
          Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.