ST1200 Anti-Ubiquitin Mouse mAb (FK2)

ST1200
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      A Broad Range Of Species M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      ST1200-100UG
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          Glass bottle 100 μg
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          Description
          OverviewRecognizes K29-, K48-, and K63-linked polyubiquitinylated and monoubiquitinylated proteins, but not free ubiquitin, in Raji cells.
          Catalogue NumberST1200
          Brand Family Calbiochem®
          Application Data
          Detection of ubiquitin by immunoblotting. Samples: K48-linked ubiquitin chains (lanes A and D), K29-linked ubiquitin chains (lanes B and E), and K63-linked ubiquitin chains (lanes C and F). Primary antibodies: Anti-Ubiquitin (recognizing only polyubiquitinylated proteins) (1:1000) (lanes A-C) and Anti-Ubiquitin Mouse mAb (FK2) (Cat. No. ST1200) (1:1000) (lanes D-F). Detection: chemiluminescence.
          References
          ReferencesMatsumoto, M., et al. 2005. Proteomics 5, 4545.
          Boname, J.N., et al. 2001. Immunity 15, 627.
          Campbell, V., et al. 2001. Neuron 32, 1013.
          Fujimuro, M., et al. 2003. FEBS Letts. 349, 173.
          Product Information
          FormLiquid
          FormulationIn PBS.
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesELISA Takada, K., et al. 1995. Eur. J. Biochem. 233, 42. Takada, K., et al. 1997. Clin. chem. 43, 1188. Immunoprecipitation Takada, K., et al. 2001. Biochem. J. 356, 199. Boname, J.N. and Stevenson, P.G. 2001. Immunity 15, 627. Matsumoto, M., et al. 2005. Proteomics 5, 4145. Immunofluorescence Campbell, D.S. and Holt, C.E. 2001. Neuron 32, 1013.
          Key Applications Immunoprecipitation
          Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunofluorescence
          Application NotesELISA (see application references)
          Immunoblotting (1:1000)
          Immunoprecipitation (see application references and comments)
          Immunofluorescence (see application references)
          Application CommentsDoes not recognize free ubiquitin. For immunoprecipitation, once the antibody is immobilized, it demonstrates affinity for free ubiquitin as well as for poly- and monoubiquitinylated proteins. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenunpurified polyubiquitinylated lysozyme
          Immunogenhuman
          CloneFK2
          HostMouse
          IsotypeIgG₁
          SourceRaji cells
          Species Reactivity
          • A Broad Range Of Species
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Store diluted antibody at 4°C for up to 1 month.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-Ubiquitin Mouse mAb (FK2) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-Ubiquitin Mouse mAb (FK2) Certificates of Analysis

          TitleLot Number
          ST1200

          References

          Reference overview
          Matsumoto, M., et al. 2005. Proteomics 5, 4545.
          Boname, J.N., et al. 2001. Immunity 15, 627.
          Campbell, V., et al. 2001. Neuron 32, 1013.
          Fujimuro, M., et al. 2003. FEBS Letts. 349, 173.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision02-March-2009 JSW
          ApplicationELISA (see application references)
          Immunoblotting (1:1000)
          Immunoprecipitation (see application references and comments)
          Immunofluorescence (see application references)
          Application Data
          Detection of ubiquitin by immunoblotting. Samples: K48-linked ubiquitin chains (lanes A and D), K29-linked ubiquitin chains (lanes B and E), and K63-linked ubiquitin chains (lanes C and F). Primary antibodies: Anti-Ubiquitin (recognizing only polyubiquitinylated proteins) (1:1000) (lanes A-C) and Anti-Ubiquitin Mouse mAb (FK2) (Cat. No. ST1200) (1:1000) (lanes D-F). Detection: chemiluminescence.
          DescriptionMouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with myeloma cells. Recognizes K29-, K48-, and K63-linked polyubiquitinylated and monoubiquitinylated proteins.
          HostMouse
          Immunogen specieshuman
          Immunogenunpurified polyubiquitinylated lysozyme
          CloneFK2
          IsotypeIgG₁
          Speciesa broad range of species
          FormLiquid
          FormulationIn PBS.
          Concentration Label Please refer to vial label for lot-specific concentration
          SourceRaji cells
          Preservative≤0.1% sodium azide
          CommentsDoes not recognize free ubiquitin. For immunoprecipitation, once the antibody is immobilized, it demonstrates affinity for free ubiquitin as well as for poly- and monoubiquitinylated proteins. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Store diluted antibody at 4°C for up to 1 month.
          Toxicity Standard Handling
          ReferencesMatsumoto, M., et al. 2005. Proteomics 5, 4545.
          Boname, J.N., et al. 2001. Immunity 15, 627.
          Campbell, V., et al. 2001. Neuron 32, 1013.
          Fujimuro, M., et al. 2003. FEBS Letts. 349, 173.
          Application referencesELISA Takada, K., et al. 1995. Eur. J. Biochem. 233, 42. Takada, K., et al. 1997. Clin. chem. 43, 1188. Immunoprecipitation Takada, K., et al. 2001. Biochem. J. 356, 199. Boname, J.N. and Stevenson, P.G. 2001. Immunity 15, 627. Matsumoto, M., et al. 2005. Proteomics 5, 4145. Immunofluorescence Campbell, D.S. and Holt, C.E. 2001. Neuron 32, 1013.