573822 Anti-Synaptophysin Mouse mAb (SP15)

573822
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service
      View Pricing & Availability

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      B, Fe, Ht, H, M, Porcine, R M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      573822-50UL
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          Glass bottle 50 ul
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          OverviewRecognizes the ~38 kDa synaptophysin protein in brain tissue. Does not cross-react with the ~65 kDa form in mouse liver extracts.
          Catalogue Number573822
          Brand Family Calbiochem®
          Application Data
          Detection of rat synaptophysin by immunoblotting. Sample: Whole tissue extract from rat liver. Primary antibody: Anti-Synaptophysin Mouse mAb (SP15) (Cat. No. 573822) (1:10,000). Detection: chemiluminescence.
          References
          ReferencesAlder, J., et al. 1995. J. Neurosci. 15, 511.
          Thomas, L., et al. 1988. Science 242, 1050.
          Rehmm, H., et al. 1986. EMBO J. 5, 5353.
          Product Information
          FormLiquid
          FormulationIn PBS, 50% glycerol, pH 7.2.
          Negative controlMouse liver extract
          Positive controlRat liver tissue
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesFrozen Sections Honer, W.G., et al. 1993. Brain Res. 609, 9.
          Key Applications Immunoprecipitation
          Frozen Sections
          Immunoblotting (Western Blotting)
          Immunocytochemistry
          Application NotesFrozen Sections (1:10; see application references)
          Immunoblotting (1:2000, colorimetric)
          Immunocytochemistry (1:250)
          Immunoprecipitation (1:100)
          Application CommentsVariables associated with assay conditions will dictate the proper working dilutions.
          Biological Information
          Immunogensynaptic vesicle-containing fractions from human brain homogenates
          ImmunogenHuman
          CloneSP15
          HostMouse
          IsotypeIgM
          Species Reactivity
          • Bovine
          • Feline
          • Hamster
          • Human
          • Mouse
          • Porcine
          • Rat
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-Synaptophysin Mouse mAb (SP15) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-Synaptophysin Mouse mAb (SP15) Certificates of Analysis

          TitleLot Number
          573822

          References

          Reference overview
          Alder, J., et al. 1995. J. Neurosci. 15, 511.
          Thomas, L., et al. 1988. Science 242, 1050.
          Rehmm, H., et al. 1986. EMBO J. 5, 5353.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision20-February-2008 JSW
          ApplicationFrozen Sections (1:10; see application references)
          Immunoblotting (1:2000, colorimetric)
          Immunocytochemistry (1:250)
          Immunoprecipitation (1:100)
          Application Data
          Detection of rat synaptophysin by immunoblotting. Sample: Whole tissue extract from rat liver. Primary antibody: Anti-Synaptophysin Mouse mAb (SP15) (Cat. No. 573822) (1:10,000). Detection: chemiluminescence.
          DescriptionAmmonium sulfate purified (50%) mouse monoclonal antibody. Recognizes the ~38 kDa syaptophysin protein.
          BackgroundSynaptophysin is a ~38 kDa integral membrane protein on synaptic vesicles. This protein consists of four transmembrane domains, with its amino- and carboxyl-terminus facing the cytoplasm. Due to its transmembrane domain structure, synaptophysin has been postulated to be a gap junction-like protein as voltage-dependent channel activity is seen when synaptophysin hexamers are reconstituted into lipid membranes. Physiological evidence for synaptophysin’s role in secretion includes its involvement in calcium-dependent glutamate secretion in Xenopus oocytes injected with rat cerebellum mRNA.
          HostMouse
          Immunogen speciesHuman
          Immunogensynaptic vesicle-containing fractions from human brain homogenates
          CloneSP15
          IsotypeIgM
          Speciesbovine, feline, hamster, human, mouse, porcine, rat
          Positive controlRat liver tissue
          Negative controlMouse liver extract
          FormLiquid
          FormulationIn PBS, 50% glycerol, pH 7.2.
          Preservative≤0.1% sodium azide
          CommentsVariables associated with assay conditions will dictate the proper working dilutions.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesAlder, J., et al. 1995. J. Neurosci. 15, 511.
          Thomas, L., et al. 1988. Science 242, 1050.
          Rehmm, H., et al. 1986. EMBO J. 5, 5353.
          Application referencesFrozen Sections Honer, W.G., et al. 1993. Brain Res. 609, 9.