DR1057 Anti-SQSTM1 Mouse mAb (2C11)

DR1057
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      DR1057-100UG
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      Discontinued
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          100 μg
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          Description
          OverviewRecognizes the SQSTM1 protein HeLa cells.
          Catalogue NumberDR1057
          Brand Family Calbiochem®
          SynonymsAnti-Sequestosome 1
          Application Data
          Detection of human SQSTM1 by immunoblotting. Sample: Full-length, recombinant human protein. Primary antibody: Anti-SQSTM1 Mouse mAb (2C11) (Cat. No. DR1057) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human SQSTM1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-SQSTM1 Mouse mAb (2C11) (Cat. No. DR1057) (10 µg/ml). Detection: fluorescence.
          References
          ReferencesLee, H,K., et al. 2010. Immunity. 32, 227.
          Bjorkoy, G., et al. 2009. Methods Enzymol. 452, 181.
          Ferguson, C,J., Lenk, G,M., and Meisler, M,H., Hum. Mol. Genet. 18 4868.
          Gal, J., et al. 2009. J. Neurochem. 111 1062.
          Mardakheh, F,K., et al. 2009.J. Cell Biol. 187, 265.
          Jung, H,S., et al. 2008. Cell Metab. 8, 318.
          Product Information
          FormLiquid
          FormulationIn PBS, pH 7.2.
          Positive controlHeLa cells
          PreservativeNone
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunocytochemistry
          Application NotesELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Immunocytochemistry (10 µg/ml)
          Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          ImmunogenFull-length, recombinant human SQSTM1 (aa 1-441), expressed as a GST protein
          ImmunogenNA
          Clone2C11
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Human
          • Mouse
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Regulatory Review
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-SQSTM1 Mouse mAb (2C11) SDS

          Title

          Safety Data Sheet (SDS) 

          References

          Reference overview
          Lee, H,K., et al. 2010. Immunity. 32, 227.
          Bjorkoy, G., et al. 2009. Methods Enzymol. 452, 181.
          Ferguson, C,J., Lenk, G,M., and Meisler, M,H., Hum. Mol. Genet. 18 4868.
          Gal, J., et al. 2009. J. Neurochem. 111 1062.
          Mardakheh, F,K., et al. 2009.J. Cell Biol. 187, 265.
          Jung, H,S., et al. 2008. Cell Metab. 8, 318.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision01-November-2010 RFH
          SynonymsAnti-Sequestosome 1
          ApplicationELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Immunocytochemistry (10 µg/ml)
          Application Data
          Detection of human SQSTM1 by immunoblotting. Sample: Full-length, recombinant human protein. Primary antibody: Anti-SQSTM1 Mouse mAb (2C11) (Cat. No. DR1057) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human SQSTM1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-SQSTM1 Mouse mAb (2C11) (Cat. No. DR1057) (10 µg/ml). Detection: fluorescence.
          DescriptionPurified mouse monoclonal antibody. Recognizes the SQSTM1 protein.
          BackgroundSQSTM1 is a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-κB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-κB in response to upstream signals.
          HostMouse
          Immunogen speciesNA
          ImmunogenFull-length, recombinant human SQSTM1 (aa 1-441), expressed as a GST protein
          Clone2C11
          IsotypeIgG2a
          Specieshuman, mouse
          Positive controlHeLa cells
          FormLiquid
          FormulationIn PBS, pH 7.2.
          PreservativeNone
          CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Regulatory Review
          ReferencesLee, H,K., et al. 2010. Immunity. 32, 227.
          Bjorkoy, G., et al. 2009. Methods Enzymol. 452, 181.
          Ferguson, C,J., Lenk, G,M., and Meisler, M,H., Hum. Mol. Genet. 18 4868.
          Gal, J., et al. 2009. J. Neurochem. 111 1062.
          Mardakheh, F,K., et al. 2009.J. Cell Biol. 187, 265.
          Jung, H,S., et al. 2008. Cell Metab. 8, 318.