NA18 Anti-Replication Protein A (Ab-2) Mouse mAb (RPA34-19)

NA18
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      Ht, H M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      NA18-100UG
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          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes the ~34 kDa unphosphorylaed and the complexed (phosphorylated) subunit of replication protein A in HeLa and U293 cells and breast carcinoma tissue.
          Catalogue NumberNA18
          Brand Family Calbiochem®
          SynonymsAnti-RP-A
          References
          ReferencesDin, S., et al. 1990. Genes Dev. 4, 968.
          Brill, S.J. and Stillman, B. 1989. Nature 342, 92.
          Stillman, B. 1989. Annu. Rev. Cell. Biol. 5, 197.
          Tsurimoto, T. and Stillman, B. 1989. EMBO J. 8, 3883.
          Wobbe, C.R., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 1834.
          Product Information
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
          Positive controlHeLa or U293 cells or breast carcinoma tissue
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Paraffin Sections
          Immunoblotting (Western Blotting)
          Immunofluorescence
          Immunoprecipitation
          Application NotesImmunoblotting (5 µg/ml)
          Immunofluorescence (1-5 µg/ml)
          Immunoprecipitation (1 µg/reaction)
          Paraffin Sections (2.5-5 µg/ml, see comments)
          Application CommentsThis antibody can be used as a marker for proliferation. Will stain paraffin sections without pre-treatment, but pre-treating with trypsin or a pressure cooker enhances staining. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenreplication protein A purified from U293 cells
          ImmunogenHuman
          EpitopeWithin the p34 subunit of replication protein A
          CloneRPA34-19
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Hamster
          • Human
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-Replication Protein A (Ab-2) Mouse mAb (RPA34-19) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-Replication Protein A (Ab-2) Mouse mAb (RPA34-19) Certificates of Analysis

          TitleLot Number
          NA18

          References

          Reference overview
          Din, S., et al. 1990. Genes Dev. 4, 968.
          Brill, S.J. and Stillman, B. 1989. Nature 342, 92.
          Stillman, B. 1989. Annu. Rev. Cell. Biol. 5, 197.
          Tsurimoto, T. and Stillman, B. 1989. EMBO J. 8, 3883.
          Wobbe, C.R., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 1834.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision04-September-2007 RFH
          SynonymsAnti-RP-A
          ApplicationImmunoblotting (5 µg/ml)
          Immunofluorescence (1-5 µg/ml)
          Immunoprecipitation (1 µg/reaction)
          Paraffin Sections (2.5-5 µg/ml, see comments)
          DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~34 kDa phosphorylated subunit and the nonphosphorylated RPA complex.
          BackgroundThe replication system of a cell contains several essential components among which are replication factor A (RFA, RPA, or HeLa cell SSB), PCNA, and replication factor C (RFC) as well as several other proteins. RPA, a multi-subunit protein containing a 70 kDa, 34 kDa, and 11 kDa protein, may play a critical regulatory role in replication based on its functioning during both the initiation and elongation stages of DNA replication. The protein is highly conserved, having been identified in a range of species from yeast (Saccharomyces cerevisiae) to humans. The primary function of RPA appears to be as an auxiliary protein for both DNA polymerase α and δ. RPA undergoes a cell cycle dependent phosphorylation, which is restricted to the p34 protein. In addition to existing as part of the multi-subunit structure, p34 has also been identified as being present in a free, unphosphorylated form. Recent data shows that RPA is restricted to the nucleus and is overexpressed in transformed cells as well as in several tumor cells.
          HostMouse
          Immunogen speciesHuman
          Immunogenreplication protein A purified from U293 cells
          EpitopeWithin the p34 subunit of replication protein A
          CloneRPA34-19
          IsotypeIgG2a
          Specieshamster, human
          Positive controlHeLa or U293 cells or breast carcinoma tissue
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsThis antibody can be used as a marker for proliferation. Will stain paraffin sections without pre-treatment, but pre-treating with trypsin or a pressure cooker enhances staining. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesDin, S., et al. 1990. Genes Dev. 4, 968.
          Brill, S.J. and Stillman, B. 1989. Nature 342, 92.
          Stillman, B. 1989. Annu. Rev. Cell. Biol. 5, 197.
          Tsurimoto, T. and Stillman, B. 1989. EMBO J. 8, 3883.
          Wobbe, C.R., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 1834.