553571 Anti-Ras (31-43) Rabbit pAb

553571
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      B, H, M, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      553571-50UG
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          Plastic ampoule 50 μg
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          Description
          OverviewRecognizes all forms of wild type Ras, including the mutant G12V Ras protein.
          Catalogue Number553571
          Brand Family Calbiochem®
          SynonymsAnti-Ras GDP/GTP Binding Protein
          Application Data
          Detection of rat Ras by immunoblotting. Sample: Whole tissue extract from rat brain. Primary antibody: Anti-Ras (31-43) Rabbit pAb (Cat. No. 553571) (1 µg/ml). Detection: chemiluminescence.
          References
          ReferencesHardie, G. and Hanks, S. 1995. in The Protein Kinase Facts Book, Protein-Serine Kinases p. 418, Academic Press, San Diego, CA.
          Sawada, M., et al. 1989. Nucleic Acid Res. 17, 8867.
          Product Information
          FormLiquid
          FormulationIn PBS, 50% glycerol, pH 7.2.
          Positive controlRat brain tissue
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Application NotesImmunoblotting (1 µg/ml)
          Application CommentsBy immmunoblotting, this anti-pan-Ras polyclonal antibody reacts strongly with recombinant Ras fusion protein as well as with recombinant glutathione S-transferase (GST) human Ras and [G12V]GST human Ras. Does not cross-react with members of the Rho or Rab families of Ras-related small G-proteins. No specific reactivity is detected by immunoblotting with rat, mouse or human cell lines or tissue extracts. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogena synthetic peptide [EYDPTIEDSYRKQ(C)] corresponding to amino acids 31-43 in the effector binding Loop (L2) of human H-, K-, and N-Ras, conjugated to KLH
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Bovine
          • Human
          • Mouse
          • Rat
          Antibody TypePolyclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-Ras (31-43) Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-Ras (31-43) Rabbit pAb Certificates of Analysis

          TitleLot Number
          553571

          References

          Reference overview
          Hardie, G. and Hanks, S. 1995. in The Protein Kinase Facts Book, Protein-Serine Kinases p. 418, Academic Press, San Diego, CA.
          Sawada, M., et al. 1989. Nucleic Acid Res. 17, 8867.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision27-January-2009 RFH
          SynonymsAnti-Ras GDP/GTP Binding Protein
          ApplicationImmunoblotting (1 µg/ml)
          Application Data
          Detection of rat Ras by immunoblotting. Sample: Whole tissue extract from rat brain. Primary antibody: Anti-Ras (31-43) Rabbit pAb (Cat. No. 553571) (1 µg/ml). Detection: chemiluminescence.
          DescriptionImmunoaffinity rabbit polyclonal antibody. Recognizes all forms of wild type Ras and the mutant G12V Ras protein.
          BackgroundRas proteins bind GDP and GTP with high affinity. This region of Ras is important in the binding of Ras to its effector, Raf1. This sequence (residues 31-43) is nearly 100% conserved between the different Ras encoded proteins (H-, K-, and N-Ras) and through phylogeny from slime mold to man.
          HostRabbit
          Immunogena synthetic peptide [EYDPTIEDSYRKQ(C)] corresponding to amino acids 31-43 in the effector binding Loop (L2) of human H-, K-, and N-Ras, conjugated to KLH
          IsotypeIgG
          Speciesbovine, human, mouse, rat
          Positive controlRat brain tissue
          FormLiquid
          FormulationIn PBS, 50% glycerol, pH 7.2.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsBy immmunoblotting, this anti-pan-Ras polyclonal antibody reacts strongly with recombinant Ras fusion protein as well as with recombinant glutathione S-transferase (GST) human Ras and [G12V]GST human Ras. Does not cross-react with members of the Rho or Rab families of Ras-related small G-proteins. No specific reactivity is detected by immunoblotting with rat, mouse or human cell lines or tissue extracts. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesHardie, G. and Hanks, S. 1995. in The Protein Kinase Facts Book, Protein-Serine Kinases p. 418, Academic Press, San Diego, CA.
          Sawada, M., et al. 1989. Nucleic Acid Res. 17, 8867.