PC130 Anti-Rad51 (Ab-1) Rabbit pAb

PC130
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      PC130-100UL
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      Stocked 
      Discontinued
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      Available
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          Contact Customer Service

          Plastic ampoule 100 ul
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          Description
          OverviewRecognizes the ~43 kDa Rad51 protein in ovarian tissue.
          Catalogue NumberPC130
          Brand Family Calbiochem®
          Application Data
          Detection of human RAD51 by immunoblotting. Sample: Whole cell lysate(10 µg) from HeLa cells. Primary antibody: Anti-RAD51 Rabbit pAb (Cat. No. PC130) (1:2500). Detection: chemiluminescence.

          Detection of human RAD51 by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate (500 µg) from HeLa cells. Antibody for IP: Anti-RAD51 Rabbit pAb (Cat. No. PC130) (40 µl). Antibody for IB: Anti-RAD51 Rabbit pAb (Cat. No. PC130) (1:2500). Detection: chemiluminescence.

          Detection of human RAD51 by immunocytochemistry. Samples: A431 and HeLa cells. Primary antibody: Anti-RAD51 Rabbit pAb (Cat. No. PC130) (1:500). Detection: fluorescence (red) with DAPI counterstain (blue). Actin filaments were stained with Alexa Fluor® 488 dye -Phalloidin (green).
          References
          ReferencesBrendel, V., et al. 1997. J. Mol. Evol. 44, 528.
          Boulikas, T., et al. 1997. Anticancer Res. 17, 843.
          Gupta, R.C., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 463.
          Mizuta, R., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 6927.
          Sharan, S.K., et al. 1997. Nature 386, 304.
          Li, M.J., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 10222.
          Shen, Z., et al. 1996. J. Biol. Chem. 271, 148.
          Sturzbecher, H.W., et al. 1996. EMBO J. 15, 1992.
          Tashiro, S., et al. 1996. Oncogene 12, 2165.
          Ashley, T., et al. 1995. Chromosoma 104, 19.
          Haaf, T., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 2298.
          Product Information
          FormLiquid
          FormulationIn 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4
          Positive controlA431 cells, HeLa cells
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Immunocytochemistry
          Immunoprecipitation
          Application NotesImmunoblotting (1:2500)
          Immunoprecipitation (40 µl/500µg total protein from lysate)
          Immunocytochemistry (1:500)
          Application CommentsDue to a high degree of sequence identity, this antibody is also expected to cross-react with bovine, equine, and canine RAD51. Antibody should be titrated for individual systems.
          Biological Information
          Immunogenrecombinant, human Rad51
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-Rad51 (Ab-1) Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-Rad51 (Ab-1) Rabbit pAb Certificates of Analysis

          TitleLot Number
          PC130

          References

          Reference overview
          Brendel, V., et al. 1997. J. Mol. Evol. 44, 528.
          Boulikas, T., et al. 1997. Anticancer Res. 17, 843.
          Gupta, R.C., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 463.
          Mizuta, R., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 6927.
          Sharan, S.K., et al. 1997. Nature 386, 304.
          Li, M.J., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 10222.
          Shen, Z., et al. 1996. J. Biol. Chem. 271, 148.
          Sturzbecher, H.W., et al. 1996. EMBO J. 15, 1992.
          Tashiro, S., et al. 1996. Oncogene 12, 2165.
          Ashley, T., et al. 1995. Chromosoma 104, 19.
          Haaf, T., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 2298.

          Citations

          Title
          Viera et al. 2009. J. cell Science 122, 2149.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision18-June-2015 JSW
          ApplicationImmunoblotting (1:2500)
          Immunoprecipitation (40 µl/500µg total protein from lysate)
          Immunocytochemistry (1:500)
          Application Data
          Detection of human RAD51 by immunoblotting. Sample: Whole cell lysate(10 µg) from HeLa cells. Primary antibody: Anti-RAD51 Rabbit pAb (Cat. No. PC130) (1:2500). Detection: chemiluminescence.

          Detection of human RAD51 by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate (500 µg) from HeLa cells. Antibody for IP: Anti-RAD51 Rabbit pAb (Cat. No. PC130) (40 µl). Antibody for IB: Anti-RAD51 Rabbit pAb (Cat. No. PC130) (1:2500). Detection: chemiluminescence.

          Detection of human RAD51 by immunocytochemistry. Samples: A431 and HeLa cells. Primary antibody: Anti-RAD51 Rabbit pAb (Cat. No. PC130) (1:500). Detection: fluorescence (red) with DAPI counterstain (blue). Actin filaments were stained with Alexa Fluor® 488 dye -Phalloidin (green).
          DescriptionAffinity purified rabbit polyclonal antibody. Recognizes the ~43 kDa Rad51 protein.
          BackgroundThe bacterial RecA and the yeast RAD51 proteins are involved in homologous recombination and DNA repair. The human RAD51 protein is the homologue of these two proteins. While the modular structure of these proteins is conserved and the homology is very strong with the bacterial protein there are functional differences seen with RAD51 including an ATP hydrolysis rate 1/10 that seen with RecA. Although RAD51 is a nuclear protein and is largely absent from the cytoplasm and nucleoli, the protein lacks a nuclear localization signal and requires other proteins in a complex to be translocated into the nucleus. Treatment of cells with DNA synthesis inducing agents will cause an increase in the levels of RAD51 protein which then undergo a shift in distribution becoming focal in nature. This focal distribution may be blocked by inhibitors of transcription. RAD51 can be shown to interact directly with the chromosomes and during the process of meiosis, its distribution of RAD51 undergoes dynamic change. RAD51 has been shown to interact with a wide variety of different proteins that include the wild-type form of p53, BRCA2, and RAD52, which specifically modulates its activity.
          HostRabbit
          Immunogen speciesHuman
          Immunogenrecombinant, human Rad51
          IsotypeIgG
          Specieshuman, mouse, rat
          Positive controlA431 cells, HeLa cells
          FormLiquid
          FormulationIn 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4
          Preservative≤0.1% sodium azide
          CommentsDue to a high degree of sequence identity, this antibody is also expected to cross-react with bovine, equine, and canine RAD51. Antibody should be titrated for individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesBrendel, V., et al. 1997. J. Mol. Evol. 44, 528.
          Boulikas, T., et al. 1997. Anticancer Res. 17, 843.
          Gupta, R.C., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 463.
          Mizuta, R., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 6927.
          Sharan, S.K., et al. 1997. Nature 386, 304.
          Li, M.J., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 10222.
          Shen, Z., et al. 1996. J. Biol. Chem. 271, 148.
          Sturzbecher, H.W., et al. 1996. EMBO J. 15, 1992.
          Tashiro, S., et al. 1996. Oncogene 12, 2165.
          Ashley, T., et al. 1995. Chromosoma 104, 19.
          Haaf, T., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 2298.
          CitationViera et al. 2009. J. cell Science 122, 2149.