OP21 Anti-Pan-Ras (Ab-1) Mouse mAb (F132-62)

OP21
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      OP21-100UG
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          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes the ~21 kDa H-, K-, and N-Ras proteins including both c- and v- Ras in SW480 and Y1 cells and normal skin tissue.
          Catalogue NumberOP21
          Brand Family Calbiochem®
          References
          ReferencesRodenhuis, S. et al., 1992 Cancer Res. (Suppl) 52, 2665s.
          Sidransky, D., et al., 1992 Science 256, 102.
          Bos, J.L., 1988 Mutation Res. 195, 255.
          Furth, M. E., et al., 1987. Oncogene 1, 47.
          Kraus, M.H., et al., 1984 Proc. Natl. Acad. Sci. USA 81, 5384.
          Shimizu, K., et al., 1983 Proc. Natl. Acad. Sci. USA 80, 2112.
          Taparowsky, E., et al.,1983 Cell 34, 581.
          Ellis, R.W., et al., 1981 Nature 292, 506.
          Shih, T.Y., et al., 1980 Nature 287, 686.
          Product Information
          FormLiquid
          FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
          Positive controlSw480 or Y1 cells or normal skin tissue
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Not Western Blot
          Paraffin Sections
          Frozen Sections
          Immunofluorescence
          Immunoprecipitation
          Application NotesFrozen Sections (5 µg/ml)
          Immunoblotting (not recommended)
          Immunofluorescence (2 µg/ml)
          Immunoprecipitation (4 µg/sample)
          Paraffin Sections (5 µg/ml, saponin or pepsin pre-treatment required)
          Application CommentsRecognizes the p21 translational products of the H-, K- and N-ras human, as well as other mammalian c- and v-ras oncogenes. Recommended for formalin-fixed and paraffin-embedded tissue sections while Anti-Pan-Ras (Ab-2) Mouse mAb (F111-85) (Cat. No. OP22) is recommended for immunoblotting. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenrecombinant p21 Ras
          Epitopewithin the N-terminus
          CloneF132-62
          HostMouse
          IsotypeIgG2b
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-Pan-Ras (Ab-1) Mouse mAb (F132-62) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-Pan-Ras (Ab-1) Mouse mAb (F132-62) Certificates of Analysis

          TitleLot Number
          OP21

          References

          Reference overview
          Rodenhuis, S. et al., 1992 Cancer Res. (Suppl) 52, 2665s.
          Sidransky, D., et al., 1992 Science 256, 102.
          Bos, J.L., 1988 Mutation Res. 195, 255.
          Furth, M. E., et al., 1987. Oncogene 1, 47.
          Kraus, M.H., et al., 1984 Proc. Natl. Acad. Sci. USA 81, 5384.
          Shimizu, K., et al., 1983 Proc. Natl. Acad. Sci. USA 80, 2112.
          Taparowsky, E., et al.,1983 Cell 34, 581.
          Ellis, R.W., et al., 1981 Nature 292, 506.
          Shih, T.Y., et al., 1980 Nature 287, 686.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision02-October-2007 RFH
          ApplicationFrozen Sections (5 µg/ml)
          Immunoblotting (not recommended)
          Immunofluorescence (2 µg/ml)
          Immunoprecipitation (4 µg/sample)
          Paraffin Sections (5 µg/ml, saponin or pepsin pre-treatment required)
          DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63 Ag8.653 mouse myeloma cells. Recognizes the ~21 kDa N-, K-, H-, and v-ras proteins.
          BackgroundThe human ras gene family consists of three identified members, H-, K- and N-ras, encoding proteins of 188-189 amino acids and 21,000 (p21) molecular weight. Human H- and K-ras are the homologues of v-H- and v-K-ras sequences originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. Normal human cellular ras genes can be activated to oncogenes by mutations occurring in codons 12, 13 and 61; such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of ras proto-oncogene proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21s and their relevance to human disease diagnosis and prognosis remains to be determined.
          HostMouse
          Immunogenrecombinant p21 Ras
          Epitopewithin the N-terminus
          CloneF132-62
          IsotypeIgG2b
          Specieshuman, mouse, rat
          Positive controlSw480 or Y1 cells or normal skin tissue
          FormLiquid
          FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsRecognizes the p21 translational products of the H-, K- and N-ras human, as well as other mammalian c- and v-ras oncogenes. Recommended for formalin-fixed and paraffin-embedded tissue sections while Anti-Pan-Ras (Ab-2) Mouse mAb (F111-85) (Cat. No. OP22) is recommended for immunoblotting. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesRodenhuis, S. et al., 1992 Cancer Res. (Suppl) 52, 2665s.
          Sidransky, D., et al., 1992 Science 256, 102.
          Bos, J.L., 1988 Mutation Res. 195, 255.
          Furth, M. E., et al., 1987. Oncogene 1, 47.
          Kraus, M.H., et al., 1984 Proc. Natl. Acad. Sci. USA 81, 5384.
          Shimizu, K., et al., 1983 Proc. Natl. Acad. Sci. USA 80, 2112.
          Taparowsky, E., et al.,1983 Cell 34, 581.
          Ellis, R.W., et al., 1981 Nature 292, 506.
          Shih, T.Y., et al., 1980 Nature 287, 686.