454578 Anti-Pan-NMDA NR1 Mouse mAb (R1JHL)

454578
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      R M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      454578-15UG
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      Stocked 
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          Plastic ampoule 15 μg
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          Description
          OverviewRecognizes the ~115 kDa NMDA receptor NR1 subunit in rat cortex tissue extracts.
          Catalogue Number454578
          Brand Family Calbiochem®
          References
          ReferencesLaurie, D.J., and Seeburg, P.H. 1994. J. Neurosci. 14, 3180
          Ishii, T., et al. 1993. J. Biol. Chem. 268, 2836.
          Monyer, H., et al. 1992. Science 256, 1217.
          Product Information
          FormLyophilized
          FormulationLyophilized from cell culture supernatant.
          Positive controlRat cortex tissue extracts
          PreservativeNone
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Immunoprecipitation
          Application NotesImmunoblotting (1:300, colorimetric)
          Immunoprecipitation (3 µg/200 µg lysate)
          Application CommentsDoes not cross-react with other NMDAR subunits. For immunoblotting, dilute in TBST containing 5% nonfat milk and 0.05% sodium azide. Antibody may be used up to 5 times in immunoblotting experiments without significant loss in signal strength. Store diluted antibody at 4°C between uses. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogena recombinant fusion protein consisting of amino acids 1-564 of rat NMDA NR1
          ImmunogenRat
          CloneR1JHL
          HostMouse
          IsotypeIgG
          Species Reactivity
          • Rat
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsReconstitute in 50 µl PBS (137 mM NaCl, 7.5 mM Na₂HPO₄, 2.7 mM KCl, 1.5 mM KH₂P0₄, pH 7.4)Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          454578

          References

          Reference overview
          Laurie, D.J., and Seeburg, P.H. 1994. J. Neurosci. 14, 3180
          Ishii, T., et al. 1993. J. Biol. Chem. 268, 2836.
          Monyer, H., et al. 1992. Science 256, 1217.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision05-August-2008 JSW
          ApplicationImmunoblotting (1:300, colorimetric)
          Immunoprecipitation (3 µg/200 µg lysate)
          DescriptionMouse monoclonal antibody lyophilized from cell culture supernatant. Recognizes the ~115 kDa subunit of the NMDAR (N-methyl-D-aspartate receptor) protein.
          BackgroundThe rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. NR1 can form NMDA-activated channels when expressed in oocytes, but the currents are larger when combined with one or more of the NMDAR2 (NR2 A-D) subunits.
          HostMouse
          Immunogen speciesRat
          Immunogena recombinant fusion protein consisting of amino acids 1-564 of rat NMDA NR1
          CloneR1JHL
          IsotypeIgG
          Speciesrat
          Positive controlRat cortex tissue extracts
          FormLyophilized
          FormulationLyophilized from cell culture supernatant.
          PreservativeNone
          CommentsDoes not cross-react with other NMDAR subunits. For immunoblotting, dilute in TBST containing 5% nonfat milk and 0.05% sodium azide. Antibody may be used up to 5 times in immunoblotting experiments without significant loss in signal strength. Store diluted antibody at 4°C between uses. Variables associated with assay conditions will dictate the proper working dilution.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsReconstitute in 50 µl PBS (137 mM NaCl, 7.5 mM Na₂HPO₄, 2.7 mM KCl, 1.5 mM KH₂P0₄, pH 7.4)Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
          Toxicity Standard Handling
          ReferencesLaurie, D.J., and Seeburg, P.H. 1994. J. Neurosci. 14, 3180
          Ishii, T., et al. 1993. J. Biol. Chem. 268, 2836.
          Monyer, H., et al. 1992. Science 256, 1217.