ST1203 Anti-PGC-1α Mouse mAb (1F3.9)

ST1203
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      ST1203-100UL
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      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          100 ul
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          Description
          OverviewRecognizes the ~113 kDa PGC-1α protein in COS cells.
          Catalogue NumberST1203
          Brand Family Calbiochem®
          Application Data
          Detection of mouse PGC-1α by immunohistochemistry. Sample: Brown adipose tissue, fixed with acetone/ethanol, from a mouse held at room temperature (left and center panels) and from a mouse held at 4°C for 5 hr (right panel). Primary antibody: Anti-PGC-1α Mouse mAb (1F3.9) (Cat. No. ST1203) (1 µg/ml) (center and right panel only; left panel stained with secondary alone). Detection: DAB.

          Detection of mouse PGC-1α by immunohistochemistry. Sample: Brown adipose tissue, fixed with acetone/ethanol, from a mouse held at room temperature (left and center panels) and from a mouse held at 4°C for 5 hr (right panel). Primary antibody: Anti-PGC-1α Mouse mAb (1F3.9) (Cat. No. ST1203) (1 µg/ml) (center and right panel only; left panel stained with secondary alone). Detection: fluorescence.

          Detection of mouse PGC-1α by immunoblotting. Sample: Whole tissue extract from brown adipose tissue of mice held at room temperature (left 3 lanes) and mice held at 4°C for 5 hr (right three lanes). Primary antibody: Anti-PGC-1α Mouse mAb (1F3.9) (Cat. No. ST1203) (1 µg/ml). Detection: chemiluminescence.
          References
          ReferencesChang, J.S., et al. 2010. J. Biol. Chem. 285, 18039.
          Zhang, Y. et al. 2009. J. Biol. Chem. 284, 32813.
          Lai, L., et al. 2008. Genes Dev. 14, 1948.
          Rodgers, J.T., et al. 2008. FEBS Lett. 582, 46.
          Mazzucotelli, A., et al. 2007. Diabetes 10, 2467.
          Nemoto, S., et al. 2005. J. Biol. Chem. 16, 16456.
          Product Information
          FormLiquid
          FormulationIn 50 mM PBS
          Positive controlBrown adipose tissue from mice exposed to cold
          PreservativeNone
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Immunohistochemistry
          Immunoprecipitation
          Application NotesImmunoblotting (1 µg/ml)
          Immunocytochemistry (5-10 µg/ml)
          Immunohistochemistry (5-10 µg/ml)
          Immunoprecipitation (1:100)
          Application CommentsAntibody should be titrated for optimal results in individual systems.
          Biological Information
          ImmunogenA recombinant protein consisting of amino acids 1-120 of mouse PGC-1α
          ImmunogenMouse
          Clone1F3.9
          HostMouse
          IsotypeIgG
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Regulatory Review
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-PGC-1α Mouse mAb (1F3.9) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-PGC-1α Mouse mAb (1F3.9) Certificates of Analysis

          TitleLot Number
          ST1203

          References

          Reference overview
          Chang, J.S., et al. 2010. J. Biol. Chem. 285, 18039.
          Zhang, Y. et al. 2009. J. Biol. Chem. 284, 32813.
          Lai, L., et al. 2008. Genes Dev. 14, 1948.
          Rodgers, J.T., et al. 2008. FEBS Lett. 582, 46.
          Mazzucotelli, A., et al. 2007. Diabetes 10, 2467.
          Nemoto, S., et al. 2005. J. Biol. Chem. 16, 16456.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision02-February-2011 RFH
          ApplicationImmunoblotting (1 µg/ml)
          Immunocytochemistry (5-10 µg/ml)
          Immunohistochemistry (5-10 µg/ml)
          Immunoprecipitation (1:100)
          Application Data
          Detection of mouse PGC-1α by immunohistochemistry. Sample: Brown adipose tissue, fixed with acetone/ethanol, from a mouse held at room temperature (left and center panels) and from a mouse held at 4°C for 5 hr (right panel). Primary antibody: Anti-PGC-1α Mouse mAb (1F3.9) (Cat. No. ST1203) (1 µg/ml) (center and right panel only; left panel stained with secondary alone). Detection: DAB.

          Detection of mouse PGC-1α by immunohistochemistry. Sample: Brown adipose tissue, fixed with acetone/ethanol, from a mouse held at room temperature (left and center panels) and from a mouse held at 4°C for 5 hr (right panel). Primary antibody: Anti-PGC-1α Mouse mAb (1F3.9) (Cat. No. ST1203) (1 µg/ml) (center and right panel only; left panel stained with secondary alone). Detection: fluorescence.

          Detection of mouse PGC-1α by immunoblotting. Sample: Whole tissue extract from brown adipose tissue of mice held at room temperature (left 3 lanes) and mice held at 4°C for 5 hr (right three lanes). Primary antibody: Anti-PGC-1α Mouse mAb (1F3.9) (Cat. No. ST1203) (1 µg/ml). Detection: chemiluminescence.
          DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~113 kDa PGC-1α protein.
          BackgroundPGC-1α is a co-activator of PPARα, PPARγ, and other transcription factors that regulates the transcriptonal program of adaptive thermogenesis in brown adipose tissue, hepatic/renal gluconeogenesis, and muscle fiber type switching. The full length protein is detected at 113 kDa and is induced in BAT by cold exposure, in liver and kidney by fasting, and in skeletal muscle by exercise. Alternative splicing of the full length gene produces a 270 aa N-terminal splice variant that migrates at ~38 kDa and is induced by the same physiological signals which induce expression of the full length protein.
          HostMouse
          Immunogen speciesMouse
          ImmunogenA recombinant protein consisting of amino acids 1-120 of mouse PGC-1α
          Clone1F3.9
          IsotypeIgG
          Specieshuman, mouse, rat
          Positive controlBrown adipose tissue from mice exposed to cold
          FormLiquid
          FormulationIn 50 mM PBS
          PreservativeNone
          CommentsAntibody should be titrated for optimal results in individual systems.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Regulatory Review
          ReferencesChang, J.S., et al. 2010. J. Biol. Chem. 285, 18039.
          Zhang, Y. et al. 2009. J. Biol. Chem. 284, 32813.
          Lai, L., et al. 2008. Genes Dev. 14, 1948.
          Rodgers, J.T., et al. 2008. FEBS Lett. 582, 46.
          Mazzucotelli, A., et al. 2007. Diabetes 10, 2467.
          Nemoto, S., et al. 2005. J. Biol. Chem. 16, 16456.