506107 | Anti-P2X7 Receptor (576-595) Rabbit pAb

506107
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      506107-1SET
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      Stocked 
      Discontinued
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      Available
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          Description
          OverviewRecognizes the P2X7 receptor protein. Supplied with a control peptide.
          Catalogue Number506107
          Brand Family Calbiochem®
          Application Data
          Detection of rat P2X7 by immunoblotting. Samples: Whole tissue extract from rat hippocampus left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-P2X7 Receptor (576-595) Rabbit pAb (Cat. No. 506107) (1:200). Detection: chemiluminescence.
          References
          ReferencesCollo, G., et al. 1997. Neuropharmacology 36, 1277.
          Surprenant, A., et al. 1996. Science 272, 735.
          Product Information
          FormLyophilized
          Formulation50 µl antibody lyophilized from PBS, 1% BSA, pH 7.4 and 40 µg lyophilized control peptide (immunogen).
          Preservative≤0.1% sodium azide (antibody only)
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Application NotesImmunoblotting (1:200-1:300, chemilimunescence)
          Application CommentsThe immunogen sequence is highly conserved in mouse and human P2X7 (18/20 and 16/20 identical amino acids, respectively). For use of control peptide as a negative control, preincubate 1 µg with 1 µg antibody for 1 h at room temperature. To minimize non-specific staining, centrifuge all antibody preparations at 10,000 x g for 5 min immediately before use to remove any aggregates. Variables associated with assay conditions will dictate the optimal working dilution.
          Biological Information
          Immunogena synthetic peptide [(C)KIRKEFPKTQGQYSGFKYPY] corresponding to amino acids 576-595 of rat P2X7 receptor, conjugated to receptor protein
          ImmunogenRat
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsReconstitute antibody with 50 µl deionized H₂O. Further dilutions should be made in a buffer with a carrier protein such as BSA (1-3%). Dissolve control peptide in deionized water. Reconstitute the control peptide with 100 µl H₂O. Following reconstitution, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          506107

          References

          Reference overview
          Collo, G., et al. 1997. Neuropharmacology 36, 1277.
          Surprenant, A., et al. 1996. Science 272, 735.

          Citations

          Title
        • Marianela Masin, et al. (2006) Fe65 interacts with P2X2 subunits at excitatory synapses and modulates receptor function. Journal of Biological Chemistry 281, 4100-4108.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision08-August-2007 RFH
          ApplicationImmunoblotting (1:200-1:300, chemilimunescence)
          Application Data
          Detection of rat P2X7 by immunoblotting. Samples: Whole tissue extract from rat hippocampus left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-P2X7 Receptor (576-595) Rabbit pAb (Cat. No. 506107) (1:200). Detection: chemiluminescence.
          DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the P2X7 receptor protein. Supplied with control peptide (immunogen).
          HostRabbit
          Immunogen speciesRat
          Immunogena synthetic peptide [(C)KIRKEFPKTQGQYSGFKYPY] corresponding to amino acids 576-595 of rat P2X7 receptor, conjugated to receptor protein
          IsotypeIgG
          Speciesrat
          FormLyophilized
          Formulation50 µl antibody lyophilized from PBS, 1% BSA, pH 7.4 and 40 µg lyophilized control peptide (immunogen).
          Preservative≤0.1% sodium azide (antibody only)
          CommentsThe immunogen sequence is highly conserved in mouse and human P2X7 (18/20 and 16/20 identical amino acids, respectively). For use of control peptide as a negative control, preincubate 1 µg with 1 µg antibody for 1 h at room temperature. To minimize non-specific staining, centrifuge all antibody preparations at 10,000 x g for 5 min immediately before use to remove any aggregates. Variables associated with assay conditions will dictate the optimal working dilution.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsReconstitute antibody with 50 µl deionized H₂O. Further dilutions should be made in a buffer with a carrier protein such as BSA (1-3%). Dissolve control peptide in deionized water. Reconstitute the control peptide with 100 µl H₂O. Following reconstitution, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesCollo, G., et al. 1997. Neuropharmacology 36, 1277.
          Surprenant, A., et al. 1996. Science 272, 735.
          Citation
        • Marianela Masin, et al. (2006) Fe65 interacts with P2X2 subunits at excitatory synapses and modulates receptor function. Journal of Biological Chemistry 281, 4100-4108.