OP181 Anti-ΔNp73 Mouse mAb (38C674)

OP181
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      OP181-100UG
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      Stocked 
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      Available
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          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes the ~73 kDa ΔNp73 protein.
          Catalogue NumberOP181
          Brand Family Calbiochem®
          Application Data
          Detection of ΔNp73 by immunoblotting. Samples: Whole cell lysates from transfected with ΔNp73 cDNA cell line (lane A), HeLa cells (lane B) and NIH 3T3 cells (lane C) cell lysates. Primary antibody: Anti-ΔNp73 Mouse mAb (38C674) (Cat. No. OP181) (1 µg/ml). Detection: chemiluminescence.
          References
          ReferencesLevrero, M., et al. 2000 J. Cell Sci. 113, 1661.
          Yang, A., et al. 2000. Nature 404, 99.
          Ueda, Y., et al. 1999. Oncogene 18, 4993.
          Kaghad, M., et al. 1997. Cell 90, 809.
          Product Information
          FormLiquid
          FormulationIn PBS, 0.2% gelatin.
          Positive controlCell lines transfected with ΔNp73
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Application NotesImmunoblotting (1 µg/ml, chemiluminescence)
          Application CommentsDoes not cross-react with full-length p73. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic peptide (LYVGDPARHLAT) corresponding to amino acids 2-13 of human ΔNp73
          ImmunogenHuman
          Clone38C674
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-ΔNp73 Mouse mAb (38C674) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-ΔNp73 Mouse mAb (38C674) Certificates of Analysis

          TitleLot Number
          OP181

          References

          Reference overview
          Levrero, M., et al. 2000 J. Cell Sci. 113, 1661.
          Yang, A., et al. 2000. Nature 404, 99.
          Ueda, Y., et al. 1999. Oncogene 18, 4993.
          Kaghad, M., et al. 1997. Cell 90, 809.

          Citations

          Title
        • Gemma Dominguez, et al. (2006) Δ-TAp73 Upregulation Correlates With Poor Prognosis in Human Tumors: Putative In Vivo Network Involving p73 Isoforms, p53, and E2F-1. Journal of Clinical Oncology 24, 805-815.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision02-October-2007 RFH
          ApplicationImmunoblotting (1 µg/ml, chemiluminescence)
          Application Data
          Detection of ΔNp73 by immunoblotting. Samples: Whole cell lysates from transfected with ΔNp73 cDNA cell line (lane A), HeLa cells (lane B) and NIH 3T3 cells (lane C) cell lysates. Primary antibody: Anti-ΔNp73 Mouse mAb (38C674) (Cat. No. OP181) (1 µg/ml). Detection: chemiluminescence.
          DescriptionPurified mouse monoclonal antibody. Recognizes the ~73 kDa p73 protein lacking the N-terminal transactivation domain, ΔpNp73.
          Backgroundp73 is a relative of tumor suppressor protein p53. It displays a high homology with p53 and with p63, a gene implicated in the maintenance of epithelial stem cells. Significant homology between p53, p63, and p73 suggests that they may have overlapping functions involved in the regulation of gene expression. The disruption of p73 leads to hippocampal dysgenesis, hydrocephalus, chronic inflammation, and infections. Splicing variant mRNAs of p73 have been identified in MCF7, a breast carcinoma cell line. These mRNAs code for variant p73 proteins bearing distinct carboxyl-terminal structures, suggesting that this region of p73 may be important for the functions of this protein. Researchers have recently cloned a p73 variant that lacks an amino-terminal transactivation domain, called ΔNp73. The ΔNp73 message is transcribed from an alternative promoter located in intron 3.
          HostMouse
          Immunogen speciesHuman
          Immunogena synthetic peptide (LYVGDPARHLAT) corresponding to amino acids 2-13 of human ΔNp73
          Clone38C674
          IsotypeIgG₁
          Specieshuman
          Positive controlCell lines transfected with ΔNp73
          FormLiquid
          FormulationIn PBS, 0.2% gelatin.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsDoes not cross-react with full-length p73. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesLevrero, M., et al. 2000 J. Cell Sci. 113, 1661.
          Yang, A., et al. 2000. Nature 404, 99.
          Ueda, Y., et al. 1999. Oncogene 18, 4993.
          Kaghad, M., et al. 1997. Cell 90, 809.
          Citation
        • Gemma Dominguez, et al. (2006) Δ-TAp73 Upregulation Correlates With Poor Prognosis in Human Tumors: Putative In Vivo Network Involving p73 Isoforms, p53, and E2F-1. Journal of Clinical Oncology 24, 805-815.