487923 | Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3)

487923
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      A Broad Range Of Species M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      487923-50UG
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      Stocked 
      Discontinued
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          Plastic ampoule 50 μg
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          Description
          OverviewRecognizes nitrotyrosine.
          Catalogue Number487923
          Brand Family Calbiochem®
          Application Data
          Detection of nitrotyrosine by immunoblotting. Samples: Nitrotyrosine BSA (lane 1) and whole cell lysate from murine macrophages treated with peroxynitrite (lane 2). Primary antibody: Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3) (Cat. No. 487923) (1 µg/ml). Detection: chemiluminescence.
          References
          ReferencesMyatt, L., et al. 1996. Hypertension 28, 488.
          Beckman, J.S., et al. 1994. Biol. Chem. Hoppe-Seyler 375, 81.
          Product Information
          FormLiquid
          FormulationIn PBS, 50% glycerol, 0.1% BSA, 0.02% sodium azide, pH 7.2.
          Positive controlMouse macrophages treated with peroxynitrite
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Application NotesImmunoblotting (2 µg/ml)
          Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogenperoxynitrite-treated KLH
          CloneCC22.8C7.3
          HostMouse
          IsotypeIgG2b
          Species Reactivity
          • A Broad Range Of Species
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsReconstitute with H₂O to a final stock concentration of 1 mg/ml. Following reconsitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          487923

          References

          Reference overview
          Myatt, L., et al. 1996. Hypertension 28, 488.
          Beckman, J.S., et al. 1994. Biol. Chem. Hoppe-Seyler 375, 81.

          Brochure

          Title
          Nitric Oxide and Oxidative Stress Brochure

          Citations

          Title
        • Matthew Whiteman, Jia Ling Siau and Barry Halliwell. (2003) Lack of Tyrosine Nitration by Hypochlorous Acid in the Presence of Physiological Concentrations of Nitrite. Journal of Biological Chemistry 278, 8380-8384.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision13-June-2016 JSW
          ApplicationImmunoblotting (2 µg/ml)
          Application Data
          Detection of nitrotyrosine by immunoblotting. Samples: Nitrotyrosine BSA (lane 1) and whole cell lysate from murine macrophages treated with peroxynitrite (lane 2). Primary antibody: Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3) (Cat. No. 487923) (1 µg/ml). Detection: chemiluminescence.
          DescriptionPurified mouse monoclonal antibody. Recognizes nitrotyrosine-containing proteins.
          BackgroundNitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by Nitric oxide synthase. The reaction of NO with superoxide which is often present under conditions of oxidative stress, forms peroxynitrite, a strong oxidant which can nitrosate tyrosine residues on proteins. Nitrotyrosine has been shown to be present in proteins from artherosclerotic lesions of human carotid arteries and also in placenta during pre-eclampsia.
          HostMouse
          Immunogenperoxynitrite-treated KLH
          CloneCC22.8C7.3
          IsotypeIgG2b
          Speciesa broad range of species
          Positive controlMouse macrophages treated with peroxynitrite
          FormLiquid
          FormulationIn PBS, 50% glycerol, 0.1% BSA, 0.02% sodium azide, pH 7.2.
          Preservative≤0.1% sodium azide
          CommentsVariables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsReconstitute with H₂O to a final stock concentration of 1 mg/ml. Following reconsitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
          Toxicity Standard Handling
          ReferencesMyatt, L., et al. 1996. Hypertension 28, 488.
          Beckman, J.S., et al. 1994. Biol. Chem. Hoppe-Seyler 375, 81.
          Citation
        • Matthew Whiteman, Jia Ling Siau and Barry Halliwell. (2003) Lack of Tyrosine Nitration by Hypochlorous Acid in the Presence of Physiological Concentrations of Nitrite. Journal of Biological Chemistry 278, 8380-8384.