AB1529 Anti-Nitric Oxide Synthase I Antibody

AB1529
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Gp, R, Rb WB, IHC Sh Serum Polyclonal Antibody
      Description
      Catalogue NumberAB1529
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Nitric Oxide Synthase I Antibody
      Alternate Names
      • NOS I
      • bNOS
      • nNOS
      References
      Product Information
      FormatSerum
      PresentationSheep serum. Lyophilized, no preservatives. Reconstitute with 100 μL of sterile distilled water. Centrifuge to remove any residue.
      Applications
      ApplicationDetect Nitric Oxide Synthase I using this Anti-Nitric Oxide Synthase I Antibody validated for use in WB, IH.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry: 1:500-1,000

      Immunoblotting

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenA 21 amino acid peptide (1409-1429) of the neuronal form of NOS from rat cerebellum, coupled to KLH. The sequence is H-RSESIAFIEESKKDADEVFSS-NH2.
      HostSheep
      SpecificitybNOS (neuronal). Not yet tested for cross-reactivity to iNOS or eNOS. See reference below.
      Species Reactivity
      • Guinea Pig
      • Rat
      • Rabbit
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NOS1
      • nNOS
      • NC-NOS
      • N-NOS
      • IHPS1
      • bNOS
      • NOS
      • EC 1.14.13.39
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P29475 # Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter.
      COFACTOR: Heme group. & Binds 1 FAD. & Binds 1 FMN. & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.
      SIZE: 1434 amino acids; 160970 Da
      SUBUNIT: Homodimer. Interacts with DLG4; the interaction possibly being prevented by the association between NOS1 and CAPON. Forms a ternary complex with CAPON and RASD1. Forms a ternary complex with CAPON and SYN1. Interacts with ZDHHC23. Interacts with NOSIP; which may impair its synaptic location (By similarity).
      SUBCELLULAR LOCATION: Cell membrane, sarcolemma; Peripheral membrane protein. Cell projection, dendritic spine (By similarity). Note=In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines (By similarity).
      TISSUE SPECIFICITY: Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
      DOMAIN: SwissProt: P29475 The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles (By similarity).
      DISEASE: SwissProt: P29475 # Genetic variations in NOS1 gene are associated with susceptibility to infantile hypertrophic pyloric stenosis type 1 (IHPS1) [MIM:179010]. IHPS has an incidence of 1-5 per 1'000 live births in whites and a marked preponderance of males to females (4:1). IHPS is the most frequent disorder requiring surgery in the first year of life. The disorder is characterized by hypertrophy and hyperplasia of the circular muscle layer of the pylorus, leading to persistent vomiting 2-12 weeks after birth. Defective pyloric relaxation and increased pyloric smooth muscle mass have been suggested to be responsible for gastric-outlet obstruction.
      SIMILARITY: Belongs to the NOS family. & Contains 1 flavodoxin-like domain. & Contains 1 PDZ (DHR) domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20 to -70°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Nitric Oxide Synthase I Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Nitric Oxide Synthase I Antibody Certificates of Analysis

      TitleLot Number
      SHEEP ANTI- NITRIC OXIDE SYNTHASE (bNOS)3070791
      SHEEP ANTI- NITRIC OXIDE SYNTHASE (bNOS) - 32170953217095
      SHEEP ANTI- NITRIC OXIDE SYNTHASE (bNOS) -26591562659156
      SHEEP ANTI- NITRIC OXIDE SYNTHASE (bNOS) -26778472677847
      SHEEP ANTI- NITRIC OXIDE SYNTHASE (bNOS) -27284172728417
      SHEEP ANTI- NITRIC OXIDE SYNTHASE (bNOS) -27949792794979
      SHEEP ANTI- NITRIC OXIDE SYNTHASE (bNOS) -28591732859173
      SHEEP ANTI- NITRIC OXIDE SYNTHASE (bNOS) -28891362889136
      SHEEP ANTI- NITRIC OXIDE SYNTHASE (bNOS) POLYCLONAL ANTIBODY2915308
      SHEEP ANTI- NITRIC OXIDE SYNTHASE (bNOS) POLYCLONAL ANTIBODY3135425

      References

      Reference overviewApplicationSpeciesPub Med ID
      Molecular and functional diversity of GABA-A receptors in the enteric nervous system of the mouse colon.
      Seifi, M; Brown, JF; Mills, J; Bhandari, P; Belelli, D; Lambert, JJ; Rudolph, U; Swinny, JD
      The Journal of neuroscience : the official journal of the Society for Neuroscience  34  10361-78  2014

      Show Abstract
      25080596 25080596
      Enteric neuropathy evoked by repeated cisplatin in the rat.
      G Vera,M Castillo,P A Cabezos,A Chiarlone,M I Martín,A Gori,G Pasquinelli,G Barbara,V Stanghellini,R Corinaldesi,R De Giorgio,R Abalo
      Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society  23  2011

      Show Abstract
      21299719 21299719
      Inhibitory neuromuscular transmission to ileal longitudinal muscle predominates in neonatal guinea pigs.
      Patel BA, Dai X, Burda JE, Zhao H, Swain GM, Galligan JJ, Bian X
      Neurogastroenterol Motil  22  909-18, e236-7. Epub 2010 May 13.  2010

      Show Abstract Full Text Article
      20482699 20482699
      Differential actions of urocortins on neurons of the myenteric division of the enteric nervous system in guinea pig distal colon.
      Liu S, Ren W, Qu MH, Bishop GA, Wang GD, Wang XY, Xia Y, Wood JD
      Br J Pharmacol  159  222-36. Epub 2009 Nov 27.  2010

      Show Abstract Full Text Article
      20002096 20002096
      Localization of muscarinic receptors M1R, M2R and M3R in the human colon.
      A M Harrington,C J Peck,L Liu,E Burcher,J M Hutson,B R Southwell
      Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society  22  2010

      Show Abstract
      20146726 20146726
      Postnatal maturation of the gastrointestinal tract: a functional and immunohistochemical study in the guinea-pig ileum at weaning.
      Raquel Abalo,Gema Vera,Antonio José Rivera,Ernesto Moro-Rodríguez,María Isabel Martín-Fontelles
      Neuroscience letters  467  2009

      Show Abstract
      19819299 19819299
      Postnatal downregulation of inhibitory neuromuscular transmission to the longitudinal muscle of the guinea pig ileum.
      Bian X, Burda JE, Carrasquillo M, Galligan JJ
      Neurogastroenterol Motil  21  969-77. Epub 2009 Apr 17.  2009

      Show Abstract
      19374637 19374637
      Differential expression of canonical (classical) transient receptor potential channels in guinea pig enteric nervous system.
      Liu, S; Qu, MH; Ren, W; Hu, HZ; Gao, N; Wang, GD; Wang, XY; Fei, G; Zuo, F; Xia, Y; Wood, JD
      The Journal of comparative neurology  511  847-62  2008

      Show Abstract Full Text Article
      Guinea Pig18925632 18925632
      Characterisation of CART-containing neurons and cells in the porcine pancreas, gastro-intestinal tract, adrenal and thyroid glands.
      Wierup, N; Gunnarsdóttir, A; Ekblad, E; Sundler, F
      BMC neuroscience  8  51  2007

      Show Abstract Full Text Article
      17625001 17625001
      CART-peptide immunoreactivity in enteric nerves in patients with Hirschsprung's disease.
      A Gunnarsdóttir,N Wierup,L T Larsson,M J Kuhar,E Ekblad
      European journal of pediatric surgery : official journal of Austrian Association of Pediatric Surgery ... [et al] = Zeitschrift für Kinderchirurgie  17  2007

      Show Abstract
      17638157 17638157

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies