NE1018 Anti-Myelin Basic Protein Mouse mAb (SMI-94)

NE1018
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      Ca, H, Mk, M, Rb, R M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      NE1018-100UL
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          Plastic ampoule 100 ul
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          Description
          Overview

          This product has been discontinued.



          Recognizes the ~21 kDa myelin basic protein in rat brain tissue.

          Catalogue NumberNE1018
          Brand Family Calbiochem®
          Application Data
          Detection of rat myelin basic protein by staining frozen sections. Sample: Rat brain. Primary antibody: Anti-Myelin Basic Protein Mouse mAb (SMI-94) (Cat. No. NE1018) (1:1000). Detection: fluorescence (green) with Hoechst counterstain.
          References
          ReferencesEvers, P. and Uylings, H.B. 1997. J. Neurosci. Methods 72, 197.
          Shin, R.W., et al. 1991. Lab. Invest. 64, 693.
          Product Information
          FormLiquid
          FormulationUndiluted ascites.
          Positive controlRat brain
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunocytochemistry
          Paraffin Sections
          Enzyme-Linked Immunosorbent Assay
          Frozen Sections
          Immunoblotting (Western Blotting)
          Application NotesELISA (1:1000)
          Frozen Sections (1:1000, see comments)
          Immunoblotting (1:1000)
          Immunocytochemistry (1:1000)
          Paraffin Sections (1:1000, heat pre-treatment required, see comments)
          Application CommentsOther species have not been tested, but the antibody is expected to recognize MBP in most mammalian species. Recognizes developing and adult myelin and developing oligodendrocytes. Distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain tissue. In conjunction with Anti-Myelin Basic Protein Mouse mAb (SMI-91) (Cat. No. NE1020) and/or Anti-Myelin Basic Protein Mouse mAb (SMI-99) (Cat. No. NE1019), this antibody is useful for immunocytochemical studies on the progression of normal and pathologic myelination. Tissues can be fixed in a variety of paraformaldehyde- or formaldehyde-containing fixatives. To insure penetration of the antibody into myelin it is necessary to pre-treat frozen sections with cold methanol for 30 min or detergent. For staining paraffin sections it is recommended that de-paraffinized tissues be autoclaved in dH2O for 10 min or boiled in Tris-saline, pH 9.0, in a microwave for 15 min to expose the epitope. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenpurified human myelin basic protein
          ImmunogenHuman
          EpitopeWithin amino acids 70-89
          CloneSMI-94
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Canine
          • Human
          • Monkey
          • Mouse
          • Rabbit
          • Rat
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-Myelin Basic Protein Mouse mAb (SMI-94) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-Myelin Basic Protein Mouse mAb (SMI-94) Certificates of Analysis

          TitleLot Number
          NE1018

          References

          Reference overview
          Evers, P. and Uylings, H.B. 1997. J. Neurosci. Methods 72, 197.
          Shin, R.W., et al. 1991. Lab. Invest. 64, 693.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision01-October-2007 RFH
          ApplicationELISA (1:1000)
          Frozen Sections (1:1000, see comments)
          Immunoblotting (1:1000)
          Immunocytochemistry (1:1000)
          Paraffin Sections (1:1000, heat pre-treatment required, see comments)
          Application Data
          Detection of rat myelin basic protein by staining frozen sections. Sample: Rat brain. Primary antibody: Anti-Myelin Basic Protein Mouse mAb (SMI-94) (Cat. No. NE1018) (1:1000). Detection: fluorescence (green) with Hoechst counterstain.
          DescriptionMouse monoclonal antibody supplied as undiluted ascites. Recognizes the ~21 kDa myelin basic protein (MBP).
          BackgroundThe myelin sheath is essential for rapid and effective propagation of action potential within the axon. Mutations identified in several myelin-related genes reportedly lead to severe demyelinating neuropathies. Recent evidence also suggests that the autoimmune disease Multiple Sclerosis (MS) is mediated by T cells with specificity for antigens in the myelin sheath. Although myelin in the central nervous system (CNS) and peripheral nervous system (PNS) is structurally similar, it is chemically distinct since oligodendrocytes form myelin in the CNS whereas Schwann cells generate PNS myelin. Myelin proteolipid protein (PLP) is the predominant integral membrane protein in mammalian CNS myelin. In contrast, myelin basic protein (MBP) is localized to the myelin cytoplasm and is highly conserved among species. Both MBP and PLP mediate myelin compaction, whereas thickness is regulated by MBP. Several other myelin proteins have been identified to play distinct or partially overlapping functions in the formation and maintenance of the myelin sheath, including P0, myelin-associated glycoprotein (MAG), peripheral myelin protein (PMP22), and Connexin 32 (Cx32). The development of the myelin sheath has been a good model for understanding the mechanisms of neuron-glia interaction.
          HostMouse
          Immunogen speciesHuman
          Immunogenpurified human myelin basic protein
          EpitopeWithin amino acids 70-89
          CloneSMI-94
          IsotypeIgG₁
          Speciescanine, human, monkey, mouse, rabbit, rat
          Positive controlRat brain
          FormLiquid
          FormulationUndiluted ascites.
          Preservative≤0.1% sodium azide
          CommentsOther species have not been tested, but the antibody is expected to recognize MBP in most mammalian species. Recognizes developing and adult myelin and developing oligodendrocytes. Distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain tissue. In conjunction with Anti-Myelin Basic Protein Mouse mAb (SMI-91) (Cat. No. NE1020) and/or Anti-Myelin Basic Protein Mouse mAb (SMI-99) (Cat. No. NE1019), this antibody is useful for immunocytochemical studies on the progression of normal and pathologic myelination. Tissues can be fixed in a variety of paraformaldehyde- or formaldehyde-containing fixatives. To insure penetration of the antibody into myelin it is necessary to pre-treat frozen sections with cold methanol for 30 min or detergent. For staining paraffin sections it is recommended that de-paraffinized tissues be autoclaved in dH2O for 10 min or boiled in Tris-saline, pH 9.0, in a microwave for 15 min to expose the epitope. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesEvers, P. and Uylings, H.B. 1997. J. Neurosci. Methods 72, 197.
          Shin, R.W., et al. 1991. Lab. Invest. 64, 693.