444905 | Anti-Mast Cell Tryptase Mouse mAb (AA1)

444905
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      Ca, Fe, H, Mk M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      444905-100UG
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          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes the 32-38 kDa α- and β-isoforms of tryptase in mast cells.
          Catalogue Number444905
          Brand Family Calbiochem®
          Application Data
          Detection of human mast cell tryptase by immunohistochemistry. Sample: Human tonsil. Priamry antibody: Anti-Mast Cell Tryptase Mouse mAb (AA1) (Cat. No. 444905) (1:2000). Detection: fluorescence.
          References
          ReferencesSommerhoff, C.P., et al. 2000. Biochim. Biophys. Acta.1477, 75.
          Little, S.S., and Johnson, D.A. 1995. Biochem. J. 307, 341.
          Morgan, S.J., et al. 1991. J. Allergy Clin. Immunol. 87, 111.
          Walls, A.F., et al. 1990. J. Pathol. 162, 119.
          Wall, A.F., et al. 1990. Clin. Exp. Allergy 20, 581.
          Product Information
          FormLiquid
          FormulationIn 10 mM PBS, pH 7.4.
          Positive controlCat, dog, human, or monkey mast cells
          PreservativeNone
          Applications
          Key Applications Frozen Sections
          Immunoblotting (Western Blotting)
          Paraffin Sections
          Application NotesFrozen Sections (0.06-0.12 µg/ml)
          Immunoblotting (1 µg/ml)
          Paraffin Sections (0.06-0.12 µg/ml, heat pre-treatment required, see comments)
          Application CommentsStaining of formalin-fixed paraffin sections requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min. Variables associated with assay conditions will dictate the optimal working dilution.
          Biological Information
          Immunogennative, human mast cell, tryptase
          ImmunogenHuman
          CloneAA1
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Canine
          • Feline
          • Human
          • Monkey
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          444905

          References

          Reference overview
          Sommerhoff, C.P., et al. 2000. Biochim. Biophys. Acta.1477, 75.
          Little, S.S., and Johnson, D.A. 1995. Biochem. J. 307, 341.
          Morgan, S.J., et al. 1991. J. Allergy Clin. Immunol. 87, 111.
          Walls, A.F., et al. 1990. J. Pathol. 162, 119.
          Wall, A.F., et al. 1990. Clin. Exp. Allergy 20, 581.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision23-October-2008 JSW
          ApplicationFrozen Sections (0.06-0.12 µg/ml)
          Immunoblotting (1 µg/ml)
          Paraffin Sections (0.06-0.12 µg/ml, heat pre-treatment required, see comments)
          Application Data
          Detection of human mast cell tryptase by immunohistochemistry. Sample: Human tonsil. Priamry antibody: Anti-Mast Cell Tryptase Mouse mAb (AA1) (Cat. No. 444905) (1:2000). Detection: fluorescence.
          DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~32-38 kDa α- and β-isoforms of tryptase in mast cells.
          BackgroundMast cells are tissue bound cells that normally are distributed throughout connective tissues. They are involved in inflammatory processes as important effector and regulatory cells. Mast cells are known to accumulate at sites of inflammation, such as asthma, allergic rhinitis, and rheumatoid arthritis. Mast cells contain preformed chemical mediators including histamine, chymase, carboxypeptidase and proteolytic tryptase. Mast cells of the skin and lung contain large amounts of tryptase, suggesting this enzyme has a major role in mast cell-mediated events. Human mast cell tryptase has the potential to be used as marker for mast cell activation and as a mediator of inflammation.
          HostMouse
          Immunogen speciesHuman
          Immunogennative, human mast cell, tryptase
          CloneAA1
          IsotypeIgG₁
          Speciescanine, feline, human, monkey
          Positive controlCat, dog, human, or monkey mast cells
          FormLiquid
          FormulationIn 10 mM PBS, pH 7.4.
          Concentration Label Please refer to vial label for lot-specific concentration
          PreservativeNone
          CommentsStaining of formalin-fixed paraffin sections requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min. Variables associated with assay conditions will dictate the optimal working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesSommerhoff, C.P., et al. 2000. Biochim. Biophys. Acta.1477, 75.
          Little, S.S., and Johnson, D.A. 1995. Biochem. J. 307, 341.
          Morgan, S.J., et al. 1991. J. Allergy Clin. Immunol. 87, 111.
          Walls, A.F., et al. 1990. J. Pathol. 162, 119.
          Wall, A.F., et al. 1990. Clin. Exp. Allergy 20, 581.