444105 | Anti-Mannose 6-Phosphate Receptor Mouse mAb (2G11)

444105
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      B, H, Mk M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      444105-100UG
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      Stocked 
      Discontinued
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      Available
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          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes the ~300 kDa mannose 6-phosphate receptor protein.
          Catalogue Number444105
          Brand Family Calbiochem®
          References
          ReferencesDintzis, S., et al. 1994. J. Biol. Chem. 269, 12159.
          Dintzis, S., and Pfeffer, S. 1990. EMBO J. 9, 77.
          Product Information
          FormLiquid
          FormulationIn PBS.
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoprecipitation
          Immunoblotting (Western Blotting)
          Immunofluorescence
          Immunohistochemistry
          Application NotesImmunoblotting (1 µg/ml, non-reducing, see comments)
          Immunofluorescence (see comments)
          Immunohistochemistry (2-5 µg/ml)
          Immunoprecipitation (see comments)
          Application CommentsDoes not detect MPR in hamster (CHO) cells. Not suitable for immunoblotting using reducing conditions. This antibody has also been reported to work for immunoprecipitation and immunofluorescence. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogennative bovine cation independent (CI)-mannose 6-phosphate receptor
          ImmunogenBovine
          Epitopewithin the extracelluar domain
          Clone2G11
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Bovine
          • Human
          • Monkey
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          444105

          References

          Reference overview
          Dintzis, S., et al. 1994. J. Biol. Chem. 269, 12159.
          Dintzis, S., and Pfeffer, S. 1990. EMBO J. 9, 77.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision07-August-2007 RFH
          ApplicationImmunoblotting (1 µg/ml, non-reducing, see comments)
          Immunofluorescence (see comments)
          Immunohistochemistry (2-5 µg/ml)
          Immunoprecipitation (see comments)
          DescriptionPurified mouse monoclonal antibody. Recognizes the mannose 6-phosphate receptor protein.
          BackgroundLysosomal enzymes containing one or two mannose 6-phosphate (man6P) moieties are moved about in the cell by two distinct but interconnected cycles by means of ~300 kDa cation-independent MPRs. The biosynthetic cycle refers to the MPR transport of newly synthesized lysosomal enzymes from the trans Golgi network to late endosomes or early lysosomes. The endocytic cycle transports extracellular lysosomal enzymes from the plasma membrane via clathrin-coated vesicles to early endosomes. The entire pool of MPRs cycles between these cellular compartments every 3 hours. The steady state distribution of MPRs is predominantly late endosomes, fewer in the trans Golgi network, and ~10% at cell surface. In addition to its man6P binding activity, MPR contains a separate binding site for the type II insulin-like growth factor and is capable of binding both man6P and IGF-II simultaneously. An ~240 kDa soluble, truncated form of the extracellular domain of MPR has been detected circulating in serum that is capable of binding both ligands.
          HostMouse
          Immunogen speciesBovine
          Immunogennative bovine cation independent (CI)-mannose 6-phosphate receptor
          Epitopewithin the extracelluar domain
          Clone2G11
          IsotypeIgG2a
          Speciesbovine, not hamster, human, monkey
          FormLiquid
          FormulationIn PBS.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsDoes not detect MPR in hamster (CHO) cells. Not suitable for immunoblotting using reducing conditions. This antibody has also been reported to work for immunoprecipitation and immunofluorescence. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesDintzis, S., et al. 1994. J. Biol. Chem. 269, 12159.
          Dintzis, S., and Pfeffer, S. 1990. EMBO J. 9, 77.