IM1012 Anti-MTA3 (400-450) Rabbit pAb

IM1012
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      IM1012-50UG
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          Plastic ampoule 50 μg
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          Description
          OverviewRecognizes the ~62 kDa MTA3 protein in MCF-7 cells.
          Catalogue NumberIM1012
          Brand Family Calbiochem®
          SynonymsAnti-Metastasis-associated gene 3
          Application Data
          Lane 1: Detection of human MTA3 by immunoblotting. Samples: Nuclear extract (25 µg) from MCF-7 cells. Primary antibody: Anti-MTA3 (400-450) Rabbit pAb (Cat. No. IM1012) (1:1000). Detection: chemiluminescence. Lanes 2 and 3: Detection of human MTA3 by immunoprecipitation and immunoblotting. Samples: Whole cell lysate from MCF-7 cells (500 µg) (lane 2). Antibody for IP: Anti-MTA3 (400-450) Rabbit pAb (Cat. No. IM1012) (1:100) and immunoprecipitation control (lane 3): nonspecific rabbit IgG (1:100). Immunoblotting conditions: same as lane 1. Detection: chemiluminescence.

          Detection of human MTA3 by immunocytochemistry. Sample: Sk-Mel-28 cells fixed for 5 min with ice cold methanol and blocked with normal goat serum. Primary antibody: Anti-MTA3 (400-450) Rabbit pAb (Cat. No. IM1012) (1:300). Detection: fluorescence.
          References
          ReferencesFujita, N., et. al. 2003. Cell 113, 207.
          Kumar R. 2003. Cell 113, 142.
          Simpson, A, et.al. 2001. Gene 273, 29.
          Product Information
          FormLiquid
          FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
          Positive controlMCF7 cells
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Immunocytochemistry
          Immunoprecipitation
          Application NotesImmunoblotting (1:500-1:5000)
          Immunocytochemistry (1:300)
          Immunoprecipitation (2-10 µg/mg lysate)
          Application CommentsAntibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic peptide corresponding to amino acids within residues 400-450 of human MTA3
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          Antibody TypePolyclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Highly Toxic
          Storage +2°C to +8°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-MTA3 (400-450) Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-MTA3 (400-450) Rabbit pAb Certificates of Analysis

          TitleLot Number
          IM1012

          References

          Reference overview
          Fujita, N., et. al. 2003. Cell 113, 207.
          Kumar R. 2003. Cell 113, 142.
          Simpson, A, et.al. 2001. Gene 273, 29.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision27-August-2007 RFH
          SynonymsAnti-Metastasis-associated gene 3
          ApplicationImmunoblotting (1:500-1:5000)
          Immunocytochemistry (1:300)
          Immunoprecipitation (2-10 µg/mg lysate)
          Application Data
          Lane 1: Detection of human MTA3 by immunoblotting. Samples: Nuclear extract (25 µg) from MCF-7 cells. Primary antibody: Anti-MTA3 (400-450) Rabbit pAb (Cat. No. IM1012) (1:1000). Detection: chemiluminescence. Lanes 2 and 3: Detection of human MTA3 by immunoprecipitation and immunoblotting. Samples: Whole cell lysate from MCF-7 cells (500 µg) (lane 2). Antibody for IP: Anti-MTA3 (400-450) Rabbit pAb (Cat. No. IM1012) (1:100) and immunoprecipitation control (lane 3): nonspecific rabbit IgG (1:100). Immunoblotting conditions: same as lane 1. Detection: chemiluminescence.

          Detection of human MTA3 by immunocytochemistry. Sample: Sk-Mel-28 cells fixed for 5 min with ice cold methanol and blocked with normal goat serum. Primary antibody: Anti-MTA3 (400-450) Rabbit pAb (Cat. No. IM1012) (1:300). Detection: fluorescence.
          DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~62 kDa MTA3 protein.
          BackgroundMTA1, MTA2, and MTA3 (Metastasis-associated gene 3, ACC# AAH53631) are members of a growing novel gene family associated with highly metastatic human carcinomas. MTA3 is expressed in all tissues as both 515 aa (MTA3) and 594 aa (MTA3L) proteins found in both the nucleus and the cytoplasmic compartments. It was identified as an Estrogen receptor (ER) regulated component of the Mi/NuRD histone deacetylase transcriptional repressor. In response to estrogen, MTA3 directly targets Snail, the master regulator of the epithelial to mesenchymal transition (EMT). Regulation of E-cadherin expression due to MTA3 and ER mediated signaling of Snail expression may be an important pathway in human breast tumors. Loss of ER results in decreased MTA3 expression and tamoxifen reduces expression of MTA3 in endometrial cells.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic peptide corresponding to amino acids within residues 400-450 of human MTA3
          IsotypeIgG
          Specieshuman
          Positive controlMCF7 cells
          FormLiquid
          FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsAntibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage.
          Toxicity Highly Toxic
          ReferencesFujita, N., et. al. 2003. Cell 113, 207.
          Kumar R. 2003. Cell 113, 142.
          Simpson, A, et.al. 2001. Gene 273, 29.