IM68 Anti-MMP-2 (Ab-8) Mouse mAb (VB3)

IM68
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      IM68-100UG
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      Discontinued
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          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes the ~72 kDa latent and ~66 kDa active forms of MMP-2 in HUVEC cells and bladder, breast, and ovarian carcinoma tissue.
          Catalogue NumberIM68
          Brand Family Calbiochem®
          SynonymsAnti-Matrix Metalloproteinase 2, Anti-Gelatinase A, Anti-72 kDa Gelatinase
          Application Data
          Detection of human MMP-2 by immunoblotting. Sample: Human MMP-2. Primary antibody: Anti-MMP-2 (Ab-8) Mouse mAb (VB3) (Cat. No. IM68) (1 µg/ml). Detection: chemiluminescence.
          References
          ReferencesCottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
          Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
          Woessner, J.F., 1991. FASEB J. 5, 2145.
          Liotta, L.A. and Stetler-Stevenson, W.G. 1990. In Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1, 99.
          Product Information
          DeclarationNot available for sale in Japan.
          FormLiquid
          FormulationIn 10 mM PBS, pH 7.4.
          Positive controlConditioned medium from dexamethasone-treated HT-1080 or HUVEC cells or bladder, breast, or ovarian carcinoma tissue
          PreservativeNone
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Immunofluorescence
          Application NotesImmunoblotting (1:200-1:400, chemiluminescence)
          Immunofluorescence (4-5 µg/ml, indirect)
          Application CommentsAntibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogennative human pro-MMP-2
          ImmunogenHuman
          CloneVB3
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-MMP-2 (Ab-8) Mouse mAb (VB3) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-MMP-2 (Ab-8) Mouse mAb (VB3) Certificates of Analysis

          TitleLot Number
          IM68

          References

          Reference overview
          Cottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
          Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
          Woessner, J.F., 1991. FASEB J. 5, 2145.
          Liotta, L.A. and Stetler-Stevenson, W.G. 1990. In Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1, 99.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision03-Febuary-2009 RFH
          SynonymsAnti-Matrix Metalloproteinase 2, Anti-Gelatinase A, Anti-72 kDa Gelatinase
          ApplicationImmunoblotting (1:200-1:400, chemiluminescence)
          Immunofluorescence (4-5 µg/ml, indirect)
          Application Data
          Detection of human MMP-2 by immunoblotting. Sample: Human MMP-2. Primary antibody: Anti-MMP-2 (Ab-8) Mouse mAb (VB3) (Cat. No. IM68) (1 µg/ml). Detection: chemiluminescence.
          DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myeloma p3-X63-Ag8.653 cells. Recognizes the ~72 kDa latent and the ~66 kDa active forms of MMP-2.
          BackgroundMatrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMPs share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an ~10 kDa segment from the N-terminus and they are inhibited by tissue inhibitor of metalloproteinases (TIMP). These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in angiogenesis, arthritis, periodontitis, and metastasis. Matrix metalloproteinase-2 (MMP-2) also known by its alternative names of 72 kDa gelatinase/type IV collagenase and gelatinase A (EC 3.4.24.24) cleaves a number of substrates including gelatins, collagen types I, IV, V, VII, X, XI, and XIV, fibronectin, elastin and proteoglycan. MMP-2 is synthesized as a 631 amino acid proenzyme (~72 kDa zymogen) which is proteolytically processed at the first 80 amino acids to the 66 kDa active form. MMP-2 has been identified in a number of tissues, cells, and plasma/serum. It is secreted in a pro-enzyme form (pro-MMP-2) and is activated in vitro by organomercurials (e.g., 4aminophenylmercuric acetate, APMA). Pro-MMP-2 and pro-MMP-2/TIMP-2 complex are specifically activated in vitro by active MT1-MMPs on the surface of tumor cells. Numerous studies have shown a correlation between collagenase expression and metastatic potential and suggest that MMP-2 may be a useful marker for the diagnosis or prognosis of cancer.
          HostMouse
          Immunogen speciesHuman
          Immunogennative human pro-MMP-2
          CloneVB3
          IsotypeIgG₁
          Specieshuman
          Positive controlConditioned medium from dexamethasone-treated HT-1080 or HUVEC cells or bladder, breast, or ovarian carcinoma tissue
          FormLiquid
          FormulationIn 10 mM PBS, pH 7.4.
          Concentration Label Please refer to vial label for lot-specific concentration
          PreservativeNone
          CommentsAntibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesCottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
          Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
          Woessner, J.F., 1991. FASEB J. 5, 2145.
          Liotta, L.A. and Stetler-Stevenson, W.G. 1990. In Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1, 99.