OP146 Anti-MDM2 (Ab-6) Mouse mAb (5B10C)

OP146
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      OP146-100UG
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          Plastic ampoule 100 μg
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          Description
          Overview

          This product has been discontinued.



          Recognizes the ~90 kDa (apparent MW) MDM2 protein in A549 and MCF-7 cells and breast carcinoma tissue.

          Catalogue NumberOP146
          Brand Family Calbiochem®
          SynonymsAnti-Ubiquitin Protein Ligase, Anti-p53 Binding Protein, Ant-Murine Double Minute Chromosome-2
          Application Data
          Detection of human MDM2 by staining paraffin sections. Sample: Breast carcinoma tissue. Primary antibody: Anti-MDM2 (Ab-6) Mouse mAb (5B10C) (Cat. No. OP146) (2.5 μg/ml) Detection: DAB
          References
          ReferencesMarchetti, A., et al. 1995. J. Pathol. 175, 31.
          Barak, Y., et al. 1993. EMBO. J. 12, 461.
          Ladanyi, M., et al. 1993. Cancer Res. 53, 16.
          Leach, F. S., et al. 1993. Cancer Res. 53, 2231.
          Oliner, J. D., et al. 1993. Nature 362, 857.
          Momand, J., et al. 1992. Cell 69, 1237.
          Oliner, J. D., et al. 1992. Nature 358, 80.
          Fakharzadeh, S. S., et al. 1991. EMBO J. 10, 1565.
          Product Information
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 50% glycerol, pH 7.5.
          Positive controlA549 cells or breast carcinoma tissue
          PreservativeNone
          Applications
          Application ReferencesImmunoprecipitation Chen, J., et al. 1993. Mol Cell Biol 13, 4107.
          Key Applications Paraffin Sections
          Immunoblotting (Western Blotting)
          Immunofluorescence
          Immunoprecipitation
          Application NotesImmunoblotting (2 µg/ml)
          Immunofluorescence (1 µg/ml)
          Immunoprecipitation (1 µg/reaction, see application references)
          Paraffin Sections (2.5 µg/ml, heat pre-treatment required)
          Application CommentsAntibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenrecombinant human MDM2
          ImmunogenHuman
          Clone5B10C
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-MDM2 (Ab-6) Mouse mAb (5B10C) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-MDM2 (Ab-6) Mouse mAb (5B10C) Certificates of Analysis

          TitleLot Number
          OP146

          References

          Reference overview
          Marchetti, A., et al. 1995. J. Pathol. 175, 31.
          Barak, Y., et al. 1993. EMBO. J. 12, 461.
          Ladanyi, M., et al. 1993. Cancer Res. 53, 16.
          Leach, F. S., et al. 1993. Cancer Res. 53, 2231.
          Oliner, J. D., et al. 1993. Nature 362, 857.
          Momand, J., et al. 1992. Cell 69, 1237.
          Oliner, J. D., et al. 1992. Nature 358, 80.
          Fakharzadeh, S. S., et al. 1991. EMBO J. 10, 1565.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision02-October-2007 RFH
          SynonymsAnti-Ubiquitin Protein Ligase, Anti-p53 Binding Protein, Ant-Murine Double Minute Chromosome-2
          ApplicationImmunoblotting (2 µg/ml)
          Immunofluorescence (1 µg/ml)
          Immunoprecipitation (1 µg/reaction, see application references)
          Paraffin Sections (2.5 µg/ml, heat pre-treatment required)
          Application Data
          Detection of human MDM2 by staining paraffin sections. Sample: Breast carcinoma tissue. Primary antibody: Anti-MDM2 (Ab-6) Mouse mAb (5B10C) (Cat. No. OP146) (2.5 μg/ml) Detection: DAB
          DescriptionPurified mouse monoclonal antibody. Recognizes the ~90 kDa (apparent MW) MMDM2 protein.
          BackgroundIncreased expression of proto-oncogenes due to gene amplification can lead to cellular transformation in the absence of other mutagenic events. Recently, a novel gene termed MDM2, first identified in mouse as a small acentromeric extrachromosomal element, was shown to become oncogenic upon amplification and tumorigenic when overexpressed in NIH3T3 and Rat2 cells. The human homolog of MDM2 gene has been isolated and mapped to chromosome 12q13-q14, a region that is found to be amplified in human sarcomas. Sequence analysis of the murine, rat, and human MDM2 genes suggests that MDM2 may be involved in transcriptional regulation. MDM2 contains two putative metal-binding motifs, one of which shares similarity to known zinc finger transcriptional activators. MDM2 also contains a putative nuclear localization signal and an acidic domain of the type found in transcriptional activators. A functional role for MDM2 has recently been identified on the basis of experiments which demonstrate that MDM2 forms a stable complex with p53 in vivo. Furthermore, over-expression of MDM2 inhibits the ability of wild type p53 to stimulate expression of target genes and this inhibition appears to result from MDM2 binding to the acidic activation domain of p53, thereby preventing p53 from directly contacting the transcriptional machinery. Interestingly, wild type p53 appears to positively regulate expression of the MDM2 gene. MDM2 amplification has been observed in sarcomas and direct analysis of the MDM2 and p53 genes in sarcomas indicates that one or the other but not both of these genes is mutated in 70% of the tumors. Thus, it appears that genetic alterations in either p53 or MDM2 represent alternative mechanisms for inactivating the same growth suppressive pathway. Regulation of MDM2 expression by p53 also represents a potential feed back control of p53 function
          HostMouse
          Immunogen speciesHuman
          Immunogenrecombinant human MDM2
          Clone5B10C
          IsotypeIgG2a
          Specieshuman
          Positive controlA549 cells or breast carcinoma tissue
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 50% glycerol, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          PreservativeNone
          CommentsAntibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesMarchetti, A., et al. 1995. J. Pathol. 175, 31.
          Barak, Y., et al. 1993. EMBO. J. 12, 461.
          Ladanyi, M., et al. 1993. Cancer Res. 53, 16.
          Leach, F. S., et al. 1993. Cancer Res. 53, 2231.
          Oliner, J. D., et al. 1993. Nature 362, 857.
          Momand, J., et al. 1992. Cell 69, 1237.
          Oliner, J. D., et al. 1992. Nature 358, 80.
          Fakharzadeh, S. S., et al. 1991. EMBO J. 10, 1565.
          Application referencesImmunoprecipitation Chen, J., et al. 1993. Mol Cell Biol 13, 4107.