GR11L Anti-IGF-IR (Ab-1) Mouse mAb (αIR3)

GR11L
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      GR11L-100UG
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          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes the ~130 kDa α and the ~90 kDa β subunits of IGF-I receptor.
          Catalogue NumberGR11L
          Brand Family Calbiochem®
          SynonymsAnti-IGF-IR, Anti-Insulin-Like Growth Factor Receptor
          References
          ReferencesRoth, R. 1988. Science 239, 1269.
          Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm. 149, 276.
          Rosen, O.M. 1987. Science 257, 1452.
          Rechler, M.M., and Nissley, S.P. 1986. Hormone Res. 24, 152.
          Ullrich, A., et al. 1986. EMBO J. 5, 2503.
          Zapf, S., and Froesch, E.R. 1986. Hormone Res. 24, 121.
          Humbel, R.E. 1984. I. Chemistry in Li Hormonal proteins and peptides. Vol 12, Chap. 4 (Academic Press, New York, 1984).
          Kull, F.C., et al.1983. J. Biol. Chem. 258, 6561.
          Product Information
          FormLyophilized
          FormulationLyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA .
          Negative controlHS27 cells
          Positive controlHepG2 cells
          PreservativeNone
          Applications
          Application ReferencesNeutralization Studies Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm. 149, 276. Kull, F.C., et al.1983. J. Biol. Chem. 258, 6561. Original Clone Kull, F.C., et al.1983. J. Biol. Chem. 258, 6561.
          Key Applications Not Paraffin Sections
          Neutralization Studies
          Immunofluorescence
          Immunoprecipitation
          Not Western Blot
          Application NotesImmunofluorescence (1-5 µg/ml)
          Immunoprecipitation (1 µg/sample)
          Neutralization Studies (1 µg/ml; see application references)
          Paraffin Sections (not recommended)
          Immunoblotting (not recommended)
          Application CommentsBlocks IGF-I binding to the IGF-I receptor. May also weakly cross-react with the insulin receptor (see application references). It also inhibits the growth of MCF-7 cells in culture suggesting the IGF-I receptor may be involved in autocrine regulation of cell growth (see application references). Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenpartially purified, human placenta IGF-I receptor
          ImmunogenHuman
          CloneαIR3
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsReconstitute with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least two h. Following reconstitution, aliquot and freeze (-20°C) or refrigerate (4°C) with 0.1% sodium azide. Freezing of aliquots is recommended for long term storage of reconstituted product. Avoid freeze/thaw cycles of solutions.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-IGF-IR (Ab-1) Mouse mAb (αIR3) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-IGF-IR (Ab-1) Mouse mAb (αIR3) Certificates of Analysis

          TitleLot Number
          GR11L

          References

          Reference overview
          Roth, R. 1988. Science 239, 1269.
          Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm. 149, 276.
          Rosen, O.M. 1987. Science 257, 1452.
          Rechler, M.M., and Nissley, S.P. 1986. Hormone Res. 24, 152.
          Ullrich, A., et al. 1986. EMBO J. 5, 2503.
          Zapf, S., and Froesch, E.R. 1986. Hormone Res. 24, 121.
          Humbel, R.E. 1984. I. Chemistry in Li Hormonal proteins and peptides. Vol 12, Chap. 4 (Academic Press, New York, 1984).
          Kull, F.C., et al.1983. J. Biol. Chem. 258, 6561.

          Citations

          Title
        • Rita Nahta, et al. (2005) Insulin-like growth factor-I receptor/human epidermal growth factor receptor 2 heterodimerization contributes to trastuzumab resistance of breast cancer cells. Cancer Research 65, 11118-11128.
        • Daniel E. Syroid, et al. (1999) A role for insulin-like growth factor-I in the regulation of Schwann cell survival. Journal of Neuroscience 19, 2059-2068.
        • Donald M. Henricks, et al. (1998) Identification of insulin-like growth factor I in bovine seminal plasma and its receptor on spermatozoa: influence on sperm motility. Biology of Reproduction 59, 330-337.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision24-August-2007 RFH
          SynonymsAnti-IGF-IR, Anti-Insulin-Like Growth Factor Receptor
          ApplicationImmunofluorescence (1-5 µg/ml)
          Immunoprecipitation (1 µg/sample)
          Neutralization Studies (1 µg/ml; see application references)
          Paraffin Sections (not recommended)
          Immunoblotting (not recommended)
          DescriptionPurified mouse monoclonal antibody generated by immunizing SJL mice with the specified immunogen and fusing splenocytes with FO mouse myeloma cells (see application references). Recognizes the ~130 kDa α and the ~90 kDa β subunits of IGF-I receptor.
          BackgroundThe insulin-like growth factors (IGFs) or somatomedins are mitogenic peptides present in the plasma of human, rat, and other species. Two distinct but chemically related IGFs have been purified from human plasma: IGF-I (Somatomedin C) and IGF-II. They are single chain polypeptides with molecular weights of 7,640 and 7,471, respectively. Types I and II IGF receptors differ in their structure and in their peptide binding specificity. Type I IGF receptors are structurally homologous to insulin receptors and contain a-subunits (MW = 130,000) that bind peptide, and b-subunits (MW = 90,000), which have intrinsic tyrosine kinase activity. The protein-tyrosine kinase activity of the insulin receptor and, presumably the IGF-I receptor, is required for insulin to stimulate all of its biological responses in cells. Type II receptors are a single polypeptide (MW = 260,000), lack protein-tyrosine kinase activity, and have been shown to be identical to the mannose-6-phosphate receptor.
          HostMouse
          Immunogen speciesHuman
          Immunogenpartially purified, human placenta IGF-I receptor
          CloneαIR3
          IsotypeIgG₁
          Specieshuman
          Positive controlHepG2 cells
          Negative controlHS27 cells
          FormLyophilized
          FormulationLyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA .
          PreservativeNone
          CommentsBlocks IGF-I binding to the IGF-I receptor. May also weakly cross-react with the insulin receptor (see application references). It also inhibits the growth of MCF-7 cells in culture suggesting the IGF-I receptor may be involved in autocrine regulation of cell growth (see application references). Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsReconstitute with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least two h. Following reconstitution, aliquot and freeze (-20°C) or refrigerate (4°C) with 0.1% sodium azide. Freezing of aliquots is recommended for long term storage of reconstituted product. Avoid freeze/thaw cycles of solutions.
          Toxicity Standard Handling
          ReferencesRoth, R. 1988. Science 239, 1269.
          Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm. 149, 276.
          Rosen, O.M. 1987. Science 257, 1452.
          Rechler, M.M., and Nissley, S.P. 1986. Hormone Res. 24, 152.
          Ullrich, A., et al. 1986. EMBO J. 5, 2503.
          Zapf, S., and Froesch, E.R. 1986. Hormone Res. 24, 121.
          Humbel, R.E. 1984. I. Chemistry in Li Hormonal proteins and peptides. Vol 12, Chap. 4 (Academic Press, New York, 1984).
          Kull, F.C., et al.1983. J. Biol. Chem. 258, 6561.
          Citation
        • Rita Nahta, et al. (2005) Insulin-like growth factor-I receptor/human epidermal growth factor receptor 2 heterodimerization contributes to trastuzumab resistance of breast cancer cells. Cancer Research 65, 11118-11128.
        • Daniel E. Syroid, et al. (1999) A role for insulin-like growth factor-I in the regulation of Schwann cell survival. Journal of Neuroscience 19, 2059-2068.
        • Donald M. Henricks, et al. (1998) Identification of insulin-like growth factor I in bovine seminal plasma and its receptor on spermatozoa: influence on sperm motility. Biology of Reproduction 59, 330-337.
        • Application referencesNeutralization Studies Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm. 149, 276. Kull, F.C., et al.1983. J. Biol. Chem. 258, 6561. Original Clone Kull, F.C., et al.1983. J. Biol. Chem. 258, 6561.

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          Life Science Research > Antibodies and Assays > Primary Antibodies