386032 | Anti-Hsp70 Mouse mAb (C92F3A-5)

386032
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      A Broad Range Of Species M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      386032-50UG
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          Plastic ampoule 50 μg
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          Description
          OverviewRecognizes the ~70 kDa Hsp70 protein. Does not cross-react with Hsc70.
          Catalogue Number386032
          Brand Family Calbiochem®
          SynonymsAnti-Heat Shock Protein 70
          Application Data
          Detection of mouse Hsp70 by immunoblotting immunoblotting. Sample: Whole cell lysate from L929 cells. Primary antibody: Anti-Hsp70 Mouse mAb (C92F3A-5) (Cat. No. 386032) (1:1000). Detection: chemiluminescence.

          Detection of human Hsp70 by staining paraffin sections. Sample: Human colon cancer tissue. Primary antibody: Anti-Hsp70 Mouse mAb (C92F3A-5) (Cat. No. 386032) (1:50). Detection: colorimetric (DAB).

          Substrate binding of Hsp70 in complex with a substrate peptide.
          References
          ReferencesHang, H., and Fox, M.H. 1995. Cytometry 19, 119.
          Kilgore, J.L., et al. 1994. J. Appl. Physiol. 76, 589.
          Heufelder, A.E., et al. 1992. J. Clin. Endocrinol. Metab. 74, 724.
          Gower, D.J., et al. 1989. J. Neurosurg. 70, 605.
          Milarski, K., et al. 1989.J. Cell Biol. 108, 413.
          Vass, K., et al. 1988. Acta Neuropathologica 77, 128.
          Welch, W.J., and Suhan, J.P. 1986. J. Cell Biol. 103, 2035.
          Product Information
          FormLiquid
          FormulationIn PBS, 50% glycerol, pH 7.2.
          Positive controlL929 cells, Human colon cancer tissue
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Immunocytochemistry
          Immunoprecipitation
          Paraffin Sections
          Enzyme-Linked Immunosorbent Assay
          Flow Cytometry
          Frozen Sections
          Application NotesELISA (see comments)
          Flow Cytometry (see comments)
          Frozen Sections (5 µg/ml)
          Immunoblotting (1 µg/ml)
          Immunocytochemistry (5 µg/ml)
          Immunoprecipitation (see comments)
          Paraffin Sections (5 µg/ml)
          Application CommentsDoes not cross-react with Hsc70. Because Hsp70 is not expressed constitutively in most cells, this antibody is suitable for ascertaining whether a stress-response has occurred in the cell. Increased levels of Hsp70 expression occurs following stress even in cells that constitutively express Hsp70. This antibody has also been reported to work for ELISA, flow cytometry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
          Biological Information
          ImmunogenHsp70 from HeLa cells
          ImmunogenHuman
          CloneC92F3A-5
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • A Broad Range Of Species
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing iniital thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          386032

          References

          Reference overview
          Hang, H., and Fox, M.H. 1995. Cytometry 19, 119.
          Kilgore, J.L., et al. 1994. J. Appl. Physiol. 76, 589.
          Heufelder, A.E., et al. 1992. J. Clin. Endocrinol. Metab. 74, 724.
          Gower, D.J., et al. 1989. J. Neurosurg. 70, 605.
          Milarski, K., et al. 1989.J. Cell Biol. 108, 413.
          Vass, K., et al. 1988. Acta Neuropathologica 77, 128.
          Welch, W.J., and Suhan, J.P. 1986. J. Cell Biol. 103, 2035.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision10-June-2009 JSW
          SynonymsAnti-Heat Shock Protein 70
          ApplicationELISA (see comments)
          Flow Cytometry (see comments)
          Frozen Sections (5 µg/ml)
          Immunoblotting (1 µg/ml)
          Immunocytochemistry (5 µg/ml)
          Immunoprecipitation (see comments)
          Paraffin Sections (5 µg/ml)
          Application Data
          Detection of mouse Hsp70 by immunoblotting immunoblotting. Sample: Whole cell lysate from L929 cells. Primary antibody: Anti-Hsp70 Mouse mAb (C92F3A-5) (Cat. No. 386032) (1:1000). Detection: chemiluminescence.

          Detection of human Hsp70 by staining paraffin sections. Sample: Human colon cancer tissue. Primary antibody: Anti-Hsp70 Mouse mAb (C92F3A-5) (Cat. No. 386032) (1:50). Detection: colorimetric (DAB).

          Substrate binding of Hsp70 in complex with a substrate peptide.
          DescriptionMouse monoclonal antibody purified by ion-exchange chromatography. Recognizes the ~70 kDa Hsp70 protein.
          BackgroundThe Hsp70 family consists of multiple members, all of which bind ATP in vitro, that are localized within different intracellular compartments. Hsp70 is not usually expressed under normal growth conditions, but is expressed at high levels in cells experiencing stress. It is found in the cytosol, nucleus, and nucleolus.
          HostMouse
          Immunogen speciesHuman
          ImmunogenHsp70 from HeLa cells
          CloneC92F3A-5
          IsotypeIgG₁
          Speciesa broad range of species
          Positive controlL929 cells, Human colon cancer tissue
          FormLiquid
          FormulationIn PBS, 50% glycerol, pH 7.2.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsDoes not cross-react with Hsc70. Because Hsp70 is not expressed constitutively in most cells, this antibody is suitable for ascertaining whether a stress-response has occurred in the cell. Increased levels of Hsp70 expression occurs following stress even in cells that constitutively express Hsp70. This antibody has also been reported to work for ELISA, flow cytometry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing iniital thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesHang, H., and Fox, M.H. 1995. Cytometry 19, 119.
          Kilgore, J.L., et al. 1994. J. Appl. Physiol. 76, 589.
          Heufelder, A.E., et al. 1992. J. Clin. Endocrinol. Metab. 74, 724.
          Gower, D.J., et al. 1989. J. Neurosurg. 70, 605.
          Milarski, K., et al. 1989.J. Cell Biol. 108, 413.
          Vass, K., et al. 1988. Acta Neuropathologica 77, 128.
          Welch, W.J., and Suhan, J.P. 1986. J. Cell Biol. 103, 2035.