393206 | Anti-HNE Fluorophore Rabbit pAb

393206
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      A Broad Range Of Species Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      393206-100UL
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      Stocked 
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      Available
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          Glass bottle 100 ul
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          Description
          OverviewRecognizes the HNE-derived chromphohore of the 2:1 lysine-HNE-fluorophore.
          Catalogue Number393206
          Brand Family Calbiochem®
          SynonymsAnti-Nα-Acetyllysine-4-hydroxy-2-nonenal Fluorophore
          References
          ReferencesTsai, L., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 7975.
          Product Information
          FormLiquid
          FormulationSerum diluted 1:1 with glycerol.
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunohistochemistry
          Application NotesELISA (1:500-1:1000)
          Immunoblotting (1:1000-1:5000)
          Immunohistochemistry (1:100-1:500)
          Application CommentsDue to the small epitope size, overnight incubation with the antibody greatly increases sensitivity by immunoblotting. Determined by competitive ELISA and immunoblotting to recognize the chromophore structure of fluorophore derived from the reaction of 4-HNE with primary amines. Does not recognize 1:1 cysteine-, 1:1 histidine-, or 1:1 lysine-HNE Michael Adducts. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogena 2:1 Nα-acetyllysine-HNE fluorophore, conjugated to KLH
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • A Broad Range Of Species
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          393206

          References

          Reference overview
          Tsai, L., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 7975.

          Brochure

          Title
          Nitric Oxide and Oxidative Stress Brochure
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision20-July-2007 JSW
          SynonymsAnti-Nα-Acetyllysine-4-hydroxy-2-nonenal Fluorophore
          ApplicationELISA (1:500-1:1000)
          Immunoblotting (1:1000-1:5000)
          Immunohistochemistry (1:100-1:500)
          DescriptionRabbit polyclonal antibody supplied as diluted serum. Recognizes the HNE-derived chromophore of the 2:1 lysine-HNE fluorophore.
          HostRabbit
          Immunogena 2:1 Nα-acetyllysine-HNE fluorophore, conjugated to KLH
          IsotypeIgG
          Speciesa broad range of species
          FormLiquid
          FormulationSerum diluted 1:1 with glycerol.
          CommentsDue to the small epitope size, overnight incubation with the antibody greatly increases sensitivity by immunoblotting. Determined by competitive ELISA and immunoblotting to recognize the chromophore structure of fluorophore derived from the reaction of 4-HNE with primary amines. Does not recognize 1:1 cysteine-, 1:1 histidine-, or 1:1 lysine-HNE Michael Adducts. Variables associated with assay conditions will dictate the proper working dilution.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesTsai, L., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 7975.